Recombinant Human Heart- And Neural Crest Derivatives-Expressed Protein 2 (HAND2) Protein (GST)

Name: Recombinant Human Heart- And Neural Crest Derivatives-Expressed Protein 2 (HAND2) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-09198P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Description	Recombinant Human Heart- And Neural Crest Derivatives-Expressed Protein 2 (HAND2) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P61296
Target Symbol	HAND2
Synonyms	AI225906; AI661148 ; autonomic nervous system and neural crest derivatives-expressed protein 2; Basic helix loop helix transcription factor HAND2; bHLHa26; Class A basic helix-loop-helix protein 26; Deciduum; Deciduum heart autonomic nervous system and neural crest derivatives expressed protein 2; DHAND 2; dHAND; DHAND2; Ehand 2; Ehand2; FLJ16260; HAND 2; Hand2; HAND2_HUMAN; Heart and neural crest derivatives expressed 2; Heart and neural crest derivatives expressed protein 2; heart and neural crest derivatives expressed transcript 2; heart; Heart- and neural crest derivatives-expressed protein 2; Hed; Highly similar to dHAND M musculus; MGC125303; MGC125304; Th 2; Th2; Thing 2; Thing2
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MSLVGGFPHHPVVHHEGYPFAAAAAASRCSHEENPYFHGWLIGHPEMSPPDYSMALSYSPEYASGTANRKERRRTQSINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDLLAKDDQNGEAEAFKAEIKKTDVKEEKRKKELNEILKSTVSSNDKKTKGRTGWPQHVWALELKQ
Expression Range	1-180aa
Protein Length	Full Length of Isoform 2
Mol. Weight	47.3kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3'.
Subcellular Location	Nucleus.
Database References	HGNC: 4808 OMIM: 602407 KEGG: hsa:9464 STRING: 9606.ENSP00000352565 UniGene: Hs.388245
Tissue Specificity	Heart.

Gene Functions References

Study showed that thalidomide inhibited the TBX5/HAND2 physical interaction, and the in silico docking revealed that the same amino acids involved in the interaction of TBX5 with DNA are also involved in its binding to HAND2. Results establish a HAND2/TBX5 pathway implicated in heart development and diseases. PMID: 28469241
HAND2 mRNA and protein low expressed in endometrial carcinoma (EC) tissues, which suggested the degree of endometrial malignancy. PMID: 29767873
The results identify HAND2 loci associated with susceptibility to early onset atrial fibrillation in a Korean population. PMID: 28460022
These findings indicate that HAND2 loss-of-function mutation contributes to human CHD, perhaps via its interaction with GATA4 and NKX2.5. PMID: 26865696
HAND2 and microRNA-1 facilitated the early progress of human induced cardiomyocyte-like cells reprogramming. PMID: 28796841
this study is the first to report the association of a HAND2 loss-of-function mutation with an increased vulnerability to tetralogy of Fallot in humans, which provides novel insight into the molecular mechanism underpinning congenital heart disease PMID: 26676105
HAND2-mediated proteolysis negatively regulates the function of estrogen receptor alpha. PMID: 26166202
suggest that HAND2 plays a key role in the regulation of progestin-induced decidualization of human endometrial stromal cells. PMID: 24745730
Reduced protein expression of HAND2 in the myenteric plexus of the aganglionic segment would suggest that HAND2 was involved in the pathogenesis of Hirschsprung disease. PMID: 24210200
Increased HAND2 methylation was a feature of premalignant endometrial lesions and was seen to parallel a decrease in RNA and protein levels. PMID: 24265601
Overdosage of Hand2 causes limb and heart defects in the human chromosomal disorder partial trisomy distal 4q. PMID: 23449628
No evidence of linkage between HAND2 and CL/P was obtained. Levelss of exclusion were obtained with different inheritance models. results did not support HAND2 in CL/P PMID: 21431856
Expression analyses on both Hand2 conditionally null and hypomorphic backgrounds demonstrate that Hand2 is required for reporter activation in a gene dosage-dependent manner during sympathetic neurogenesis. PMID: 22323723
Data suggest Hand2 plays an important role in decidualization; expression of Hand2 is significantly increased in response to prostaglandin E2. PMID: 21527398
These data suggest that cytokines can inhibit norepinephrine transporter expression through downregulation of Hand2 or Gata3 in cultured sympathetic neurons, but axotomy in adult animals selectively suppresses Hand2 expression. PMID: 21241805
Hand2 performs an essential role during transgenic epicardialization, directly impacting epicardial cell differentiation and formation of the coronary vasculature. PMID: 21350214
HAND2 may be a potential candidate gene of stenosis of the right ventricle, outflow tract. PMID: 20819618
dHAND/E-protein (E2A, ME2, and ALF1) heterodimers have distinct DNA binding specificities PMID: 15351717
These results demonstrate the direct interactions of the Phox2a and b and dHAND transcription factors within a noradrenergic cell type PMID: 16280598
demonstrate evolutionarily conserved functions of HAND transcription factors in Drosophila and mammalian cardiogenesis, and reveal a previously unrecognized requirement of Hand genes in hematopoiesis PMID: 16467358
Hand2 plays a pivotal role in regulating both cell-autonomous and -nonautonomous functions of the cardiac neural crest. PMID: 19008477
Expression of HAND2 and DEIN genes in neuroblastoma is co-regulated by asymmetrical activity of this promoter and modulated by the activity of two cis-regulatory elements acting as weak repressors PMID: 19348682

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.