Recombinant Human HDAC9 Protein

Name: Recombinant Human HDAC9 Protein
Brand: Beta LifeScience
SKU: BL-0234SG
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	NM_058176
Synonym	DKFZp779K1053; HD7; HDAC; HDAC7; HDAC7B; HDAC9B; HDAC9FL; HDRP; KIAA0744; MITR
Background	HDAC9 or MITR belongs to the histone deacetylase /acuc/apha family and is a component of the histone deacetylase complex. HDAC9 gene is orthologous to the Xenopus and mouse MITR genes (MEF2-interacting transcription repressor). HDAC9 represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. HDAC9 is a calcium-sensitive transcriptional repressor of MEF2 (1). Binding of calmodulin to HDAC9 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression. HDAC9 is a signal-responsive transcriptional repressor in mouse skeletal muscle that is downregulated upon denervation (2).
Description	Recombinant human HDAC9 (548-end) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	548a.a.-end
Molecular Weight	~77 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Active
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human HDAC9 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.; Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
Subcellular Location	Nucleus.
Protein Families	Histone deacetylase family, HD type 2 subfamily
Database References	HGNC: 14065 OMIM: 606543 KEGG: hsa:9734 STRING: 9606.ENSP00000408617 UniGene: Hs.196054
Associated Diseases	A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
Tissue Specificity	Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).

Gene Functions References

This study highlights HDAC9 as a mediator of cell invasion and angiogenesis in Triple negative breast cancer (TNBC) cells through VEGF and MAPK3 by modulating miR-206 expression and suggests that selective inhibition of HDAC9 may be an efficient route for TNBC therapy. PMID: 29936177
the T allele of rs2107595 in HDAC9 increases the risk of stroke but that the G allele of rs2389995 decreases the risk of stroke in the Chinese population (Meta-Analysis) PMID: 28145521
HDAC9 may be a new indicator for assessing chronic heart failure. PMID: 30074176
HDAC9 may be involved in the process of diabetic nephropathy. PMID: 27633396
HDAC9, in cooperation with BRG1 and MALAT1, mediates a critical epigenetic pathway responsible for vascular smooth muscle cells dysfunction. PMID: 29520069
M4 macrophages are a possible source for HDAC9 and MMP12 expression in advanced human carotid plaques. PMID: 28343758
HDAC9 is an important epigenetic factor regulating ox-LDL-induced endothelial cell apoptosis and inflammatory factor expression. PMID: 27100479
decline in HDAC9c expression over time was accompanied by increased EZH2 expression. PMID: 27250566
HDAC9 might contribute to lymphomagenesis by altering pathways involved in growth and survival, as well as modulating BCL6 activity and p53 tumor suppressor function. PMID: 27799148
post-translational modification of Nkx3.2 employing HDAC9-PIASy-RNF4 axis plays a crucial role in controlling chondrocyte viability and hypertrophic maturation during skeletal development in vertebrates. PMID: 27312341
Data show that the gene encoding the transcription factor SOX9 was identified by a global transcriptomic approach as an HDAC9 target gene. PMID: 26930713
The minor allele A of SNP rs2107595 increased coronary artery disease risk and the severity of coronary atherosclerosis in a Chinese Han population. PMID: 27494404
in leiomyosarcomas (LMS), this two-faced trait of MEF2 is relevant for tumor aggressiveness. Class IIa HDACs are overexpressed in 22% of LMS, where high levels of MEF2, HDAC4 and HDAC9 inversely correlate with overall survival. The knock out of HDAC9 suppresses the transformed phenotype of LMS cells, by restoring the transcriptional proficiency of some MEF2-target loci PMID: 28419090
Based on this study, it is suggested that HDAC9 regulates the formation of APBs and could be a candidate for the target of ALT-cancer therapy. PMID: 27833022
PC3/Tis21 associates with HDAC1, HDAC4, and HDAC9 in vivo, in fibroblast cells. PMID: 27333946
HDAC9 is a target of miR-377 in oral squamous cell carcinoma. PMID: 28267394
Studied HDAC9 gene's association with an increased susceptibility to acute coronary syndrome (ACS) in Chinese Han population. The results revealed a significant association of rs2240419 with ACS risk in which the A allele (P = 0.047) and the A allele carriers (AA + AG) (P = 0.037) were more likely to be in ACS group as compared to those in the control group. PMID: 27642596
Downregulation of HDAC9 promotes gliomas. PMID: 27495233
overexpression of HDAC9 contributes to OSCC carcinogenesis via targeting a transcription factor, MEF2D, and NR4A1/Nur77, a pro-apoptotic MEF2 target PMID: 26992905
The aurora kinase A inhibited by MLN 8054 are both implicated in cell cycle progression and, thus, in cellular proliferation.Epigenetic regulators were targeted by SAHA inhibiting HDACs and by DZNep inhibiting the histone methyltransferase EZH2, which silences genes by trimethylating histone H3K27.Combinations of small molecular inhibitors act synergistically in rhabdoid tumor PMID: 27466490
These results highlighting the significant correlation between TWIST and HDAC9 gene expression suggest that both genes may contribute to plaque stability in a coordinated way PMID: 26621503
Polymorphisms of HDAC9 is associated with Ischemic Stroke. PMID: 26347468
The results imply that HDAC9 is involved in the transcriptional regulation of human odontoblasts in vivo. PMID: 22297573
HDAC9 was commonly expressed in retinoblastoma tissues and HDAC9 was overexpressed in prognostically poor retinoblastoma patients. PMID: 27033599
HDAC9 promotes tumor formation of glioblastoma via TAZ-mediated EGFR pathway activation. PMID: 25760078
the results of this study suggest that targeting HDACs by ST-3595 might represent as a novel and promising anti-pancreatic cancer strategy. PMID: 26084607
results indicate that HDAC9 variant rs2107595 may be not associated with IS risk in southern Han Chinese PMID: 26093197
Data show that miR-376a and HDAC9 expression are inversely correlated in hepatocellular carcinoma and suggest that HDAC9-mediated epigenetic modification may contribute to the down-regulation of the miR-376 cluster in hepatocellular carcinoma. PMID: 25613642
Gene expression studies in peripheral blood mononuclear cells revealed increased mRNA levels of HDAC9. Analysis of human atherosclerotic plaques revealed no association between rs2107595 and specific plaque characteristics. PMID: 25388417
The hydroxamic acid pan-HDAC inhibitor TSA synergistically inhibit the viability. PMID: 24562770
The results elucidate the genetic etiology of lung adenocarcinoma by demonstrating that SNP rs10248565 in the HDAC9 gene may be a potential biomarker of cancer susceptibility. PMID: 24650256
These results suggest that HDAC9 may be a suppressor and its downregulation might promote the progression process, especially in lung adenocarcinomas. PMID: 24427341
study reports gene expression in skeletal muscle tissue of women with metabolic syndrome is enriched in inflammatory response-related genes; IL6R, HDAC9 and CD97 expression correlated negatively with insulin sensitivity; suggests a role for these 3 inflammatory genes in development of skeletal muscle insulin resistance in women PMID: 23771909
These data suggest that HDAC9 variants may be selected for during cutaneous squamous cell carcinoma tumorigenesis PMID: 23784969
In vivo sorafenib + HDAC inhibitor toxicity against tumors was increased. PMID: 23674352
HDAC9 gene is significantly associated with large-vessel stroke risk in Chinese population. PMID: 23828597
We propose that CtBP2 is an ovarian cancer oncogene that regulates gene expression program by modulating HDAC activity. PMID: 22945647
SNP, rs12155400, in the histone deacetylase 9 gene (HDAC9) on chromosome 7, was associated with retinopathy in analysis of participants without hypertension PMID: 23393555
Our results are consistent with the 7p21.1 association acting via promoting atherosclerosis, and consistent with alterations in HDAC9 expression mediating this increased risk. PMID: 23449258
Regression models consistently identified rs2522129, rs2675231, and rs2389963 as having among the highest predictive values for explaining differences related to brain volume measures PMID: 22884548
Dephosphorylation at a conserved SP motif governs cAMP sensitivity and nuclear localization of class IIa histone deacetylases HDAC4, 5 and 9 PMID: 23297420
HDAC9 promotes angiogenesis and transcriptionally represses the endothelial cell miR-17-92 cluster. PMID: 23288173
LBH589 and TSA may translationally regulate some HDAC encoding genes in pancreatic tumors. PMID: 22487525
Data indicate a pronounced deregulation of HDAC genes HDAC9 and HDAC11 in patients with Philadelphia-negative chronic myeloproliferative neoplasms: essential thrombocythemia (ET), polycythemia vera (PV) and primary myelofibrosis (PMF). PMID: 21806350
We identified a new association for large vessel stroke within HDAC9 on chromosome 7p21.1. PMID: 22306652
Treated the seminoma-like cell line TCam-2 with the HDAC-inhibitor Depsipeptide. PMID: 21987446
The results show that hdac9 is the third androgenic alopecia susceptibility gene PMID: 22032556
HDAC9 acts as an epigenetic switch in effector T cell-mediated systemic autoimmunity. PMID: 21708950
HDAC suppresses the activation of PPARgamma in the gastric carcinoma cell line SGC-7901. PMID: 19624894
MITR plays a master switch role to balance osteogenic and adipogenic differentiation of MSCs through regulation of PPARgamma-2 transcriptional activity. PMID: 21247904

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