Recombinant Human Guanine Nucleotide-Binding Protein Subunit Alpha-15 (GNA15) Protein (His-Trx)

Name: Recombinant Human Guanine Nucleotide-Binding Protein Subunit Alpha-15 (GNA15) Protein (His-Trx)
Brand: Beta LifeScience
SKU: BLC-09249P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Guanine Nucleotide-Binding Protein Subunit Alpha-15 (GNA15) Protein (His-Trx) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P30679
Target Symbol	GNA15
Synonyms	G alpha 15; G alpha 16; G alpha-15; G alpha-16; G-protein subunit alpha-15; G-protein subunit alpha-16; GNA 15; GNA 16; GNA15; GNA15_HUMAN; GNA16; Gq class; Guanine nucleotide binding protein alpha 15; Guanine nucleotide binding protein alpha 15 subunit; Guanine nucleotide-binding protein subunit alpha-15; Guanine nucleotide-binding protein subunit alpha-16
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-Trx
Target Protein Sequence	MARSLTWRCCPWCLTEDEKAAARVDQEINRILLEQKKQDRGELKLLLLGPGESGKSTFIKQMRIIHGAGYSEEERKGFRPLVYQNIFVSMRAMIEAMERLQIPFSRPESKHHASLVMSQDPYKVTTFEKRYAAAMQWLWRDAGIRAYYERRREFHLLDSAVYYLSHLERITEEGYVPTAQDVLRSRMPTTGINEYCFSVQKTNLRIVDVGGQKSERKKWIHCFENVIALIYLASLSEYDQCLEENNQENRMKESLALFGTILELPWFKSTSVILFLNKTDILEEKIPTSHLATYFPSFQGPKQDAEAAKRFILDMYTRMYTGCVDGPEGSKKGARSRRLFSHYTCATDTQNIRKVFKDVRDSVLARYLDEINLL
Expression Range	1-374aa
Protein Length	Full Length
Mol. Weight	60.6kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.
Protein Families	G-alpha family, G(q) subfamily
Database References	HGNC: 4383 OMIM: 139314 KEGG: hsa:2769 STRING: 9606.ENSP00000262958 UniGene: Hs.73797
Tissue Specificity	Specifically expressed in hematopoietic cells. Expressed in epididymis (at protein level).

Gene Functions References

GNA15 was not expressed in normal neuroendocrine cells but was overexpressed in gastroenteropancreatic neuroendocrine neoplasia cell lines. PMID: 25701539
Data indicate the importance of Galpha16 as a downstream effector of the non-canonical Wnt signaling pathway and as a potential therapeutic target for the treatment of non small cell lung cancer. PMID: 24204697
G15 ectopic presence could functionally contribute to the transformation process since siRNA-induced depletion of Galpha15 in pancreatic carcinoma cell lines dramatically inhibited anchorage-independent growth and resistance to the lack of nutrients. PMID: 23200847
The paper describes G alpha 15 resistance to arrestin-desensitization making it differ from other heterotrimeric G proteins (Gq, Gs, Gi, G12/13 class) PMID: 19275934
This paper shows how G alpha 15 and other Gq class members phosphorylate PKD in differing patterns PMID: 22227248
This review summarizes the specific features that distinguish Galpha15/GNA15 (G15 alpha subunit) from all other alpha subunits of the Gq class. G15 is a heterotrimeric G protein complex that is named after its alpha subunit. PMID: 20150327
beta3 region of Galpha16 is essential for interaction with TPR1 and the subsequent activation of Ras PMID: 21486497
Megakaryocytopoiesis was accompanied by down-regulation of the 43 kDa and 46 kDa variants of G16alpha, constant expression of Gsalpha, and up-regulation of Gqalpha and Gialpha1/2. PMID: 11686331
role of intracellular loops of cannabinoid CB1 receptor in functional interaction with Galpha16. PMID: 12095632
G protein-coupled receptors activate STAT3 via G alpha(16), a G alpha subunit which is primarily expressed in hematopoietic cells. PMID: 12670499
Galpha16 stimulates STAT3 via a c-Src/JAK- and ERK-dependent mechanism PMID: 14551213
Results suggest that the G(q) class proteins are degraded through the proteasome pathway and that cellular localization and/or other protein interactions determine their stability. PMID: 16218966
Co-stimulation of G(s) and G(q) can result in the fine-tuning of STAT3 activation status, and this may provide the basis for cell type-specific responses following activation of hIP. PMID: 18755267
The ability of CCR1 to signal through Galpha(14/16) thus provides a linkage for chemokines to regulate NF-kappaB-dependent responses. PMID: 19687291

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.