Recombinant Human GGPS1 Protein (N-6His)

Name: Recombinant Human GGPS1 Protein (N-6His)
Brand: Beta LifeScience
SKU: BL-1284NP
Availability: InStock

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Geranylgeranyl Pyrophosphate Synthase is produced by our E.coli expression system and the target gene encoding Met1-Glu300 is expressed with a 6His tag at the N-terminus.
Accession	O95749
Synonym	Geranylgeranyl Pyrophosphate Synthase; GGPP Synthase; GGPPSase; (2E;6E)-Farnesyl Diphosphate Synthase; Dimethylallyltranstransferase; Farnesyl Diphosphate Synthase; Farnesyltranstransferase; Geranylgeranyl Diphosphate Synthase; Geranyltranstransferase; GGPS1
Gene Background	Geranylgeranyl pyrophosphate synthase (GGPS1) is a member of the FPP/GGPP synthase family. GGPS1 is highly expressed in testis, heart and skeletal muscle. GGPS1 is localized in the cytoplasm and has geranylgeranyl diphosphate (GGPP) synthase activity. It catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins. Other transcriptional splice variants have been found.
Molecular Mass	37 KDa
Apmol Mass	35 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 20% Glycerol, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins.
Subcellular Location	Cytoplasm.
Protein Families	FPP/GGPP synthase family
Database References	HGNC: 4249 OMIM: 606982 KEGG: hsa:9453 STRING: 9606.ENSP00000282841 UniGene: Hs.730768
Tissue Specificity	Abundantly expressed in testis. Found in other tissues to a lower extent.

Gene Functions References

results may support a model in which accumulation of susceptibility variants (including some in relevant genes, notably GGPS1) may lead to a possible genetic component of predisposition to atypical femoral fractures. PMID: 28467865
GGPPS1 may play a critical role during the development of HCC from cirrhosis and is of clinical significance for predicting biological character of HCC. PMID: 24716791
Egr-1 most likely does not induce the constitutive activation of Erk1/2 through its target gene GGPS1. PMID: 23478574
the Egr-1/GGPPS/Erk1/2 pathway is responsible for insulin resistance during hyperinsulinism PMID: 21321112
these results reveal a new EGR-1/GGPPS/MAPK signaling pathway that controls cigarette smoke-induced pulmonary inflammation. PMID: 21224049
Study suggested that GGPS1 -8188A ins/del polymorphism may confer susceptibility to femoral neck BMD response to bisphosphonate therapy in Korean women. PMID: 20191015
crystal structure; it reveals that three dimers join together to form a propeller-bladed hexameric molecule with a mass of approximately 200 kDa PMID: 16698791
Farnesyltranstransferase mutations are associated with lonafarnib resistance PMID: 17536018
results indicate that the active form of GGPS in the solution is an octamer rather than hexamer or dimer PMID: 17646172
Evidence for a non-membrane bound isoform of geranylgeranyl diphosphate synthase 1 in rat. PMID: 8419360

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.