Recombinant Human Gdnf Family Receptor Alpha-1 (GFRA1) Protein (His), Active

Name: Recombinant Human Gdnf Family Receptor Alpha-1 (GFRA1) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05874P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Gdnf Family Receptor Alpha-1 (GFRA1) Protein (His), Active is produced by our Mammalian cell expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	The ED50 as determined by its ability to bind Human GDNF in functional ELISA is less than 10 ug/ml.
Uniprotkb	P56159
Target Symbol	GFRA1
Synonyms	GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R; GDNF RA; GDNF receptor alpha; GDNF receptor alpha-1; GDNFR alpha 1; GDNFR alpha; GDNFR; GDNFR-alpha-1; GDNFRA; GDNFRalpha; GFR alpha 1; GFR alpha1; GFR-alpha-1; GFRA 1; Gfra1; GFRA1_HUMAN; GFRalpha1; Glial cell line derived neurotrophic factor receptor alpha; GPI linked anchor protein; MGC23045; PI linked cell surface accessory protein; RET 1L; RET ligand 1; RET1L; RETL 1; RETL1; TGF beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR 1; TRNR1
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	C-6His
Complete Sequence	DRLDCVKASDQCLKEQSCSTKYRTLRQCVAGKETNFSLASGLEAKDECRSAMEALKQKSLYNCRCKRGMKKEKNCLRIYWSMYQSLQGNDLLEDSPYEPVNSRLSDIFRVVPFISDVFQQVEHIPKGNNCLDAAKACNLDDICKKYRSAYITPCTTSVSNDVCNRRKCHKALRQFFDKVPAKHSYGMLFCSCRDIACTERRRQTIVPVCSYEEREKPNCLNLQDSCKTNYICRSRLADFFTNCQPESRSVSSCLKENYADCLLAYSGLIGTVMTPNYIDSSSLSVAPWCDCSNSGNDLEECLKFLNFFKDNTCLKNAIQAFGNGSDVTVWQPAFPVQTTTATTTTALRVKNKPLGPAGSENEIPTHVLPPCANLQAQKLKSNVSGNTHLCISNGNYEKEGLGASS
Expression Range	25-429aa
Protein Length	Full Length of Mature Protein
Mol. Weight	46.28 kDa
Research Area	Neuroscience
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20 mM PB, 150 mM NaCl, pH 7.2
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
Subcellular Location	Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus, trans-Golgi network. Endosome. Endosome, multivesicular body.
Protein Families	GDNFR family
Database References	HGNC: 4243 OMIM: 601496 KEGG: hsa:2674 STRING: 9606.ENSP00000393725 UniGene: PMID: 29037220 the methylation status of CpG sites in GFRA1 and GSTM2 may have a role and could be used as potential biomarkers for the screening of rectal cancer PMID: 27566576 GFRA1 regulates AMPK-dependent autophagy by promoting SRC phosphorylation independent of proto-oncogene RET kinase PMID: 27754745 that the consequences of this is that GFRalpha-1-mediated signalling is altered during the ageing process PMID: 27346872 RET c.1296A may be a common susceptibility allele for nephron underdosing-related diseases. The 5'-UTR and intronic variants near exon 5 of GFRA1 are not associated with nephron endowment. PMID: 27533506 Hox proteins coordinate motor neuron differentiation and connectivity programs through Ret/Gfra genes. PMID: 26904955 This study demonstrate, using a knock-in mouse model in which GFRalpha1 is no longer located in lipid rafts, that the developmental functions of GDNF in the periphery require the translocation of the GDNF receptor complex into lipid rafts. PMID: 26400951 Methylation changes of GFRA1, SRF, and ZNF382 may be a potential biomarker set for prediction of gastric carcinoma metastasis. PMID: 25009298 GFRalpha1 levels in neurons from autopsied AD brains are significantly decreased. PMID: 25253858 In the cochlea, GFRalpha-1 was identified mainly in the cell bodies of the spiral neurons. In the organ of Corti, GFRalpha-1 was demonstrated in the Deiters' cells, Hensen cells, inner pillar cells, and weakly in inner hair cells but not in the outer hair cells. PMID: 24139947 The study shows co-localization of RET with GFRA1 and GFRA2 in myenteric ganglia of the adult human colon. PMID: 23881409 The expression of GFRalpha1 and/or GFRalpha3, especially when combined with ARTN expression, may be useful predictors of disease progression and outcome in specific subtypes of mammary carcinoma. PMID: 23351331 Mutations in GFRA1 gene is associated with urinary tract malformations. PMID: 22729463 Post-synaptic transgenic GFRalpha1 has profound effects on the development of dopamine neurons, resulting in a 40% increase in the adult number. PMID: 21133924 This study found nominally-significant evidence for interactions between GFRA1, 2 and 3 associated with schizophrenia and clozapine response, consistent with the locations of these three genes within linkage regions for schizophrenia. PMID: 20116071 Results identify persephin, a GDNF family member, as a novel ligand for GFRalpha1/RET receptor complex. PMID: 20350599 MEN2 arises from activating missense mutations in RET, causing autodimerization under certain conditions. The position of missense mutations influences medullary thyroid carcinoma aggressiveness. Review. PMID: 20669561 Here we report that human nigral dopaminergic neurons express GFRalpha1 and RET receptors at all ages. There was no reduction in the number of neurons expressing these receptors as a function of age. PMID: 20347960 GFR-alpha1 mRNA transcripts were detected in oocytes and GCs from all samples from fetuses, girls and adult women. PMID: 19896648 The expression of GFRA1 in normal infants and normoganglionic colon of patients with Hirschsprung's disease was restricted to receptor tyrosine kinase(RET)-negative glial cells and RET-positive neurons of the ganglionic plexus. PMID: 12065680 GFRA1-193C > G and 537T > C could be in linkage disequilibrium with other loci responsible for medullary thyroid cancer PMID: 12490080 analysis of binding surface for the GDNF-GFR alpha 1 PMID: 14514671 GFRalpha-1 were observed within sensory and motor nuclei of cranial nerves, dorsal column nuclei, olivary nuclear complex, reticular formation, pontine nuclei, locus caeruleus, raphe nuclei, substantia nigra, and quadrigeminal plate. PMID: 17825269 GDNF can act as an important component of the inflammatory response in breast cancers and its effects aare mediated by GFR alpha 1 receptors. PMID: 18089803 GDNF is a key component to preserve several cell populations in the nervous system and also participates in the survival and differentiation of peripheral neurons. PMID: 18394855 38 cases of germ cell tumors: 26 cases contained immature teratoma, of which 24 had immature neuroepithelium and showed strong membrane staining for GFRalpha-1. staining for GFRalpha-1 in immature neuroepithelium may facilitate its identification. PMID: 19019765

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.