Recombinant Human G-CSF Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0020UL
Recombinant Human G-CSF (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at approximately 17 kDa and purity greater than 97%.
Recombinant Human G-CSF (2 µg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at approximately 17 kDa and purity greater than 97%.

Recombinant Human G-CSF Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0020UL
/
Size

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Product Overview

Product Name Recombinant Human G-CSF Protein
Target Symbol CSF3
Alternative Names Granulocyte Colony Stimulating Factor, Colony Stimulating Factor 3GCSF, CSF3, C17orf33, CSF3OS, Filgrastim, Pluripoietin, Lenograstim
Accession Number NP_757373.1
Expression System E.Coli.
Molecular Weight 18.7 kDa (monomer, predicted)
Sequence Thr31-Pro204
Purity >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using mouse M-NFS-60 cells. The ED50 for this effect is typically 10-50 pg/mL.
Formulation Lyophilized from sterile Sodium Acetate and Sodium Chloride with Trehalose.
Endotoxin Level <0.02 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function G-CSF primarily stimulates the proliferation, differentiation, and survival of neutrophilic granulocyte precursors in the bone marrow and stimulates upregulation of mature neutrophil release into the bloodstream as part of the innate immune response. During an immune response, G-CSF is secreted by monocytes, macrophages, and activated T cells. G-CSF binds to the G-CSF receptor (G-CSFR), which is expressed on the surface of hematopoietic cells, particularly myeloid progenitors. G-CSF signaling occurs via the JAK/STAT, Ras/MAPK and PI3K/Akt pathways.
Tissue Specificity G-CSF expression has been reported in bone marrow stromal cells, fibroblasts, endothelial cells, and epithelial cells.
Cellular Localization Secreted protein
Involvement In Disease Dysregulation of G-CSF is implicated in leukemias (such as acute myeloid leukemia) and myelodysplastic syndromes, autoimmune disorders (rheumatoid arthritis and inflammatory bowel disease), congenital neutropenia, and solid tumors where aberrant expression may contribute to tumor progression, metastasis, and the formation of a supportive tumor microenvironment.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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