Recombinant Human Friend Leukemia Integration 1 Transcription Factor (FLI1) Protein (His)

Name: Recombinant Human Friend Leukemia Integration 1 Transcription Factor (FLI1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-07604P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Friend Leukemia Integration 1 Transcription Factor (FLI1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q01543
Target Symbol	FLI1
Synonyms	(Proto-oncogene Fli-1)(Transcription factor ERGB)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	MDGTIKEALSVVSDDQSLFDSAYGAAAHLPKADMTASGSPDYGQPHKINPLPPQQEWINQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY
Expression Range	1-452aa
Protein Length	Full Length
Mol. Weight	57.0 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Subcellular Location	Nucleus.
Protein Families	ETS family
Database References	HGNC: 3749 OMIM: 193067 KEGG: hsa:2313 STRING: 9606.ENSP00000399985 UniGene: Hs.504281
Associated Diseases	Ewing sarcoma (ES); Bleeding disorder, platelet-type 21 (BDPLT21)

Gene Functions References

These results indicate that Fli1 deficiency promotes migration, proliferation and cell survival, while abating tube formation of endothelial cells, suggesting that Fli1 deficiency is potentially attributable to the development of both proliferative obliterative vasculopathy (occlusion of arterioles and small arteries) and destructive vasculopathy (loss of small vessels) characteristic of SSc vasculopathy. PMID: 28370536
Fli1 functioned as an oncogene in HCC carcinogenesis and it exerted its promoting metastatic effect primarily by modulating the matrix metalloproteinase (MMP)2 signaling pathway. PMID: 29138848
Decreased serum LIF levels may be associated with vasculopathy in systemic sclerosis (SSc) and that Fli1 deficiency may contribute to the inhibition of LIF-dependent biological effects on SSc endothelial cells by suppressing the expression of LIF, LIF receptor, and gp130. PMID: 29038846
The mouse (Fli1) and human Fli1 genes are similarly regulated by Ets factors in T cells. PMID: 19829305
In contrast, expression of Spi1/PU.1 in a Fli1 producing erythroleukemia cell line in which fli1 is activated, resulted in increased proliferation through activation of growth promoting proteins MAPK, AKT, cMYC and JAK2 PMID: 28586009
CXCL6 expression is upregulated by Fli1 deficiency in fibroblasts and endothelial cells, potentially contributing to the development of fibrosis and vasculopathy in the skin, lung, and heart of systemic sclerosis. PMID: 28507181
This is the first report presenting CD13 and FLI1 as important mediators of resistance to BRAF inhibition with potential as drug targets in BRAF inhibitors refractory melanoma. PMID: 29048432
This study uncovers FLI1 as an important driving factor that promotes tumor growth in SCLC through the miR-17-92 pathway. FLI1 may serve as an attractive target for therapeutic intervention of SCLC. PMID: 28410216
identification of two novel FLI1 variants (c.1010G>A and c.1033A>G) responsible for macrothrombocytopenia; study provides new insights into the phenotype, pathophysiology and diagnosis of FLI1 variant-associated thrombocytopenia PMID: 28255014
miR-145 acts as a tumor suppressor by directly reducing expression of FLI-1, and the miR-145/FLI-1 pathway is important for tumor progression in osteosarcoma PMID: 27304058
this paper provides a mechanistic insight into the regulation of angiogenesis by TLRs and confirm a central role of Fli1 in regulating vascular homeostasis. PMID: 29141862
In American cutaneous leishmaniasis caused by Leishmania braziliensis infection, MMP1 is regulated by factors other than FLI1, and the influence of IL-6 on MMP1 was independent of its effect on FLI1. PMID: 28119029
epithelial Fli1 deficiency might be involved in the systemic autoimmunity and selective organ fibrosis in systemic sclerosis (SSc). PMID: 28232470
study shows that FLI-1 is expressed variably in different subtypes of NSCLC, and its expression is related to clinicopathologic parameters and poorer prognosis PMID: 26317314
FLI1 and MMP9 position differently in prostate cancer than in normal tissue and prostate hyperplasia, whereas MMP2 is repositioned in both prostate cancer and hyperplasia. PMID: 26564800
FLI1 promoter hypermethylation is associated with Colorectal Cancer. PMID: 25472652
Concurrent exogenous expression of three transcription factors, GATA1, FLI1 and TAL1, enables large-scale production of megakaryocytes from human pluripotent stem cells. PMID: 27052461
This study for the first time identifies FLI1 as a clinically and functionally important target gene of metastasis, providing a rationale for developing FLI1 inhibitors in the treatment of breast cancer. PMID: 26156017
SLFN11 has a role as a transcriptional target of EWS-FLI1 and is a determinant of drug response in Ewing sarcoma PMID: 25779942
Fli1 is epigenetically suppressed and is a potential predisposing factor in the pathogenesis of systemic sclerosis. (Review) PMID: 26055516
erythrocyte lineage enforces exclusivity through upregulation of EKLF and its lineage-specific cytokine receptor (EpoR) while inhibiting both FLI-1 and the receptor TpoR (also known as MPL) for the opposing megakaryocyte lineage PMID: 26159733
Mutation in FLI1 is associated with Paris-Trousseau thrombocytopenia. PMID: 26316623
Fli-1 expression gradually increases in parallel with disease progression. PMID: 26305602
Simultaneous downregulation of KLF5 and Fli1 is a key feature underlying systemic sclerosis. PMID: 25504335
endothelial CCN1 downregulation at least partially due to Fli1 deficiency may contribute to the development of digital ulcers in systemic sclerosis patients PMID: 25421497
bosentan...increased DNA binding of Fli1 and the suppression of type I collagen expression in systemic sclerosis fibroblasts PMID: 24708674
The similarity among the phenotypes of EWS/FLI1- and EWS siRNA-transfected HeLa cells points to the inhibition of EWS as the key mechanism for the induction of midzone defects. PMID: 25483190
FLI1 levels are reduced in human breast cancer samples and cell lines.FLI1 expression is correlated with breast cancer cellular growth, migration, and invasion and altered gene expression. PMID: 25379017
p16 and possibly Fli-1 may have utility in the assignment of growth phase for cutaneous melanomas. PMID: 25062258
Results show that overexpression is intimately related to malignant phenotypes and poor clinical outcome of epithelial ovarian cancer. PMID: 24923303
Fli1 bound to the CXCL5 promoter and its gene silencing significantly suppressed the CXCL5 mRNA expression in human dermal microvascular endothelial cells PMID: 24292093
Report FLI1 expression in epithelioid sarcomas. PMID: 24072183
Fli1 and CTGF are important mediators of the fibrogenic actions of adenosine PMID: 23663495
these results indicate that under quiescent conditions Fli1 recruits HDAC1/p300 to the COL1A2 promoter and suppresses the expression of the COL1A2 gene by chromatin remodeling through histone deacetylation. PMID: 24058639
these results fill a gap in the literature: the confirmation that MCC is devoid of the EWS/FLI-1 rearrangement. PMID: 23165331
Overexpression of the ETS-related transcription factor ETV1 can initiate neoplastic transformation of the prostate. PMID: 23774214
alterations in FLI1 and RUNX1 may be common in patients with platelet dense granule secretion defects and mild thrombocytopenia. PMID: 24100448
a novel function for Fli-1 in T cell development and leukaemogenesis PMID: 23667468
Podoplanin and Fli-1 immunohistochemistry may be useful in distinguishing atypical fibroxanthoma from angiosarcoma. PMID: 23518636
Ciprofloxacin has antifibrotic actions in Systemic sclerosis dermal and lung fibroblasts via the downregulation of Dnmt1, the upregulation of Fli1. PMID: 23041765
In 5q- syndrome, but not Diamond-Blackfan anemia, blood & bone marrow mononuclear cells had high Fli1 mRNA levels. This protects megakaryocytic cells from ribosomal stress & permits effective though dysplastic megakaryopoiesis. PMID: 22965552
Our findings of SNP array adn phenotype correclation do not support the traditional view that FLI1 deletion is the cause of thrombocytopenia in Jacobsen syndrome. PMID: 22887642
We found that transcriptional activation of PRKCB was directly regulated by the chimeric fusion oncogene EWSR1-FLI1 that drivesewing sarcoma growth. PMID: 22930730
FLI1 is a novel ETS transcription factor involved in gene fusions in prostate cancer and that intratumor genetic heterogeneity of ETS rearrangements can occasionally be found in index primary tumors. PMID: 22081504
Data show there are no differences in the distribution of immunohistochemical reactivity for CD31, CD34, D2-40, or FLI1 between AIDS-related and non-AIDS-related Kaposi sarcoma (KS) or between nodular- and patch/plaque-stage KS. PMID: 22372906
Expression of FLI-1, ELF-1, and GABP activated the PF4 promoter in HepG2 cells. PMID: 21931859
we have demonstrated association between FLI1 and susceptibility to CL caused by L. braziliensis. PMID: 21633373
FLI1 expression is frequently abnormal and prognostically adverse in acute myeloid leukemia. PMID: 21917756
a role of Fli1 as a negative regulator of the estrogen receptor alpha gene in dermal fibroblasts. PMID: 21451544
these results demonstrate that in SSc fibroblasts, c-Abl is an upstream regulator of the profibrotic PKCdelta/phospho-Fli-1 pathway, via induction of PKCdelta nuclear localization. PMID: 21321929

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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