Recombinant Human Forkhead Box Protein P3 (FOXP3) Protein (His&Myc)

Name: Recombinant Human Forkhead Box Protein P3 (FOXP3) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-06232P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Forkhead Box Protein P3 (FOXP3) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Activity	Not tested.
Uniprotkb	Q9BZS1
Target Symbol	FOXP3
Synonyms	Scurfin
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MPNPRPGKPSAPSLALGPSPGASPSWRAAPKASDLLGARGPGGTFQGRDLRGGAHASSSSLNPMPPSQLQLPTLPLVMVAPSGARLGPLPHLQALLQDRPHFMHQLSTVDAHARTPVLQVHPLESPAMISLTPPTTATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPNPSAPRKDSTLSAVPQSSYPLLANGVCKWPGCEKVFEEPEDFLKHCQADHLLDEKGRAQCLLQREMVQSLEQQLVLEKEKLSAMQAHLAGKMALTKASSVASSDKGSCCIVAAGSQGPVVPAWSGPREAPDSLFAVRRHLWGSHGNSTFPEFLHNMDYFKFHNMRPPFTYATLIRWAILEAPEKQRTLNEIYHWFTRMFAFFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDELEFRKKR
Expression Range	1-417aa
Protein Length	Full Length of Mature Protein
Mol. Weight	53.2 kDa
Research Area	Transcription
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7. Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1. Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development. Inhibits the transcriptional activator activity of RORA. Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4.
Subcellular Location	Nucleus. Cytoplasm.
Database References	HGNC: 6106 OMIM: 300292 KEGG: hsa:50943 STRING: 9606.ENSP00000365380 UniGene: Hs.247700
Associated Diseases	Immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX)

Gene Functions References

Results are in line with the hypothesis that in the early phase of ALS, neuroprotective helper T cells infiltrate in the affected areas in the lumbar spinal cord. This was reflected in higher peripheral percentage of CD4(+) helper T cells and higher expression of FOXP3 and IL-2Ralpha. PMID: 29574662
RUNX3, a CD8(+) lineage-specific transcription factor, binds at the FOXP3-promoter to induce its transcription. PMID: 28487507
Multivariate analysis of OS only found CD8(+)/Foxp3(+) ratio to be independent prognostic factor (P = 0.022) om spinal chordoma. PMID: 29051990
Treg cells from asthmatic patients expressed more FOXP3 as well as GATA3; the expression level of GATA3 negatively correlated with FEV1%pred. Increased expressions of USP21 and PIM2 in Treg cells from asthmatic patients were found. PMID: 30013989
Authors have demonstrated that excessive amounts of STAT5 may bind more TET2 to the FOXP3-TSDR and upregulate FOXP3 expression via DNA demethylation. Study improved the mechanism of FOXP3-TSDR hypomethylation in tumor-infiltrating CD4(+) T cells of CRC patients. PMID: 30013992
Early-pregnancy decidual mRNA expression of the regulatory T-cell marker, FOXP3, was sixfold lower (p < 0.01) in pregnancies with a male fetus compared to pregnancies with a female fetus. PMID: 30003112
In conclusion we suggest that genetic variants in FOXP3 gene may contribute to the pathogenesis of preeclampsia. PMID: 29206055
Data show that the forkhead Box Protein P3 (FOXP3) response element at the -310 bp region, but not the -2182 bp region, is mainly required for ubiquitin conjugating enzyme 9 (UBC9) activation by FOXP3. PMID: 30011797
The concurrent overexpression of FoxM1 and FoxP3 was evident in gastric cancer and inversely correlated with patient survival. PMID: 29804142
Results suggest that -924A/G and +459T/C polymorphisms of the FOXP3 gene might be associated with unexplained recurrent spontaneous abortions (URSA) and -20G/A polymorphism is likely to be rare in Indian population and might not be associated with URSA. PMID: 29932060
mutations can cause early-onset insulin-requiring diabetes with or without other features of IPEX syndrome PMID: 29193502
The rs3761549, rs5902434, and rs2232365 are associated with an increased risk of acute graft-versus-host disease and decreased risk of post allogenic hematopoietic stem cell transplantation. PMID: 30027704
Genetic variations at SNP rs3761548 in the FOXP3 gene represent a risk factor for peptic ulcer. PMID: 29938865
Aberrant expression and methylation patterns of FoxP3 were detected in human T cells. PMID: 29901207
blood mononuclear cells in stable CAD patients express a ratio of FOXP3 isoforms that is characteristic for activated CD4+ T cells PMID: 29100058
this study suggested that macrophages in keloid tissues presented high activation status and were polarized toward the M2 subtype; moreover, these macrophages could promote Treg differentiation by upregulating Foxp3 expression. PMID: 29253537
FOXP3 polymorphisms may be important markers to determine susceptibility to IIP or CTP-ILD in Chinese population. PMID: 28508461
IPEX syndrome is associated with mutations in the forkhead box P3 (FOXP3) gene which causes impaired immune suppressive function. PMID: 28960390
NOTCH1 is a central mediator of TGFbetamediated FOXP3 expression and NOTCH1 inhibition produces a significant reduction of melanoma cell proliferation and viability. PMID: 29620159
The FOXP3 gene is one of the most important genes in the regulation of the immune cells. PMID: 29526847
susceptibility to RSA was subject to the synthetic regulation of chromosomal aberrations and genetic mutations within CLTA-4 and Foxp3, suggesting that the conduction of karyotype analysis and genetic detection for RSA patients could effectively guide effective RSA counseling and sound child rearing. PMID: 29476189
The ratio of cytotoxic (CD8(+) ) to regulatory (FoxP3(+) ) T-cells, in the intra-tumoral compartment, but not in the peri-tumoral compartment, can predict survival after resection of CRCLM. PMID: 29878369
Combined results from many studies suggest that rs3761548 polymorphism is associated with cancer risk. [review] PMID: 30142808
It was concluded that the abnormal expression of endometrial E2A existed in mid-secretory endometrium of women with recurrent miscarriage, and there was a positive correlation between E2A and FOXP3, and E2A and CTLA-4, suggesting the possible regulatory role of E2A in endometrium receptivity. PMID: 29270752
this study provides evidence of the association of rs2294020 with systemic sclerosis evolution in female patients, modulating the time of progression from the diagnosis of early systemic sclerosis to the diagnosis of definite systemic sclerosis while no effect on systemic sclerosis susceptibility per se was found PMID: 29030005
Lymphocytic enteritis associated to celiac disease shows an increase of FOXP3 expression and lymphocytes T-gammadelta that is not detected in other etiologies of enteritis. PMID: 28281276
Downregulated expression of FOXP3 by siRNA transfection significantly inhibited cell proliferation and enhanced chemosensitivity to cisplatin in A549 lung adenocarcinoma cells. PMID: 28935177
SNPs have a highly significant association with the risk of Graves' disease development in a Kashmiri population. PMID: 29890310
Foxp3 is associated with lymphangiogenesis of cervical cancer. PMID: 28923073
expressions in primary lesions as well as lymphogenic metastases appear to predict high-risk head and neck squamous cell carcinoma patients PMID: 28741409
Polymorphic marker -3279 C>A of the FOXP3 gene was not associated with the risk of Pulmonary Sarcoidosis patients of Russian ethnicity. PMID: 29411762
Data show that forkhead box P3 protein (FOXP3) protein not only binds with DNA sequences containing one FKH consensus sequence, but also binds with DNA sequences with two direct repeats of consensus sequences separated by three-nucleotides (DRE3). PMID: 28910978
Study identifies CCR8+ regulatory T cells (Treg cells) as drivers of immunosuppression and provides compelling evidence of a self-feeding mechanism by which, at an autoimmune site, CCL1 produced by FOXp3+ Treg cells upregulates the expression of its own receptor, CCR8, on these cells, and potentiates their in vivo proliferation and suppressive activities as driver Treg cells. PMID: 28533380
our findings suggest that FOXP3 suppresses tumor progression in hepatocellular carcinoma (HCC) via TGF-b/Smad2/3 signaling pathway, highlighting the role of FOXP3 as a prognostic factor and novel target for an optimal therapy against this fatal malignancy PMID: 28903735
Studied lymphocyte phenotype in resected lymph nodes of patients with lung cancer by analyzing levels of Foxp3 and CD8 by immunohistochemical staining. PMID: 28831395
We conclude that Foxp3 promoter polymorphisms are associated with susceptibility to endometrial cancer in Chinese Han women. PMID: 29718856
Studied the association between Foxp3 gene polymorphisms and the susceptibility to differentiated thyroid cancers in a Chinese Han population. PMID: 27892628
DLBCL cases with high FOXP3 have longer survival (P=0.03). T cells in the background of DLBCL may play a role in modulation of tumor progression. Their presence is associated with favorable prognostic parameters in DLBCL PMID: 26862953
no significant association between rs3761548, rs2232365 polymorphisms of the FOXP3 gene, and an increased susceptibility to allergic rhinitis [meta-analysis] PMID: 28741671
Zinc is capable of ameliorating the allogeneic immune reaction by enhancement of antigen-specific iTreg cells due to modulation of essential molecular targets by upregulation of Foxp3 and KLF-10 and downregulation of IRF-1. PMID: 27260002
The Foxp3+ regulatory T cells (Tregs) play an indispensable role in controlling tolerance and immunity against self- and foreign antigens. PMID: 28696819
study highlights the role of tissue differentiation on pathological response to neoadjuvant chemotherapy in gastric cancer and shows no impact between FOXP3, HER2 and MET expression in terms of tumor regression grading PMID: 29696715
Flicr, a long noncoding RNA, modulates Foxp3 expression and autoimmunity. PMID: 28396406
FOXP3 genotype association with breast cancer susceptibility and outcome depends on the breast cancer subtype. PMID: 28713192
Results showed that physical activity in urban children was associated with lower FOXP3 promoter methylation, under conditions of high black carbon (BC) exposure. Reduced FOXP3 promoter methylation was associated with higher lung function. PMID: 28630656
Findings indicate a transcriptional axis of FOXP3-BRCA1-miR-155 in breast cancer cells and show that plasma miR-155 may serve as a non-invasive biomarker for detection of early stage breast cancer. PMID: 28562349
FOXP3 can act as a co-activator to facilitate the Wnt-b-catenin signaling pathway, inducing epithelial-mesenchymal transition and tumor growth and metastasis in non-small cell lung cancer. PMID: 28716029
no significant association of FoxP3 promoter rs3761548 or (GT) n repeat length with presumed immunological graft failure. The genotype frequencies of Vav1 intron polymorphisms did not significantly differ between patients with graft failure and matched controls. PMID: 28470865
Patients with the rs3761548 CC genotype showed better graft survival than those with the AC or AA genotype. Patients with the rs3761548 CC genotype also showed a lower rate of recurrence of the original glomerular disease than those with the AC or AA genotype. The frequency of acute rejection (AR) in patients with the rs2280883 TT genotype was lower than that in patients with the rs2280883 CT or CC genotype. PMID: 28643491
we here revealed possible involvement of FOXP3 in regulating cCSC self-renewal via tuning COX2 expression, and thus providing a new target for the eradication of colon cancer stem cells. PMID: 28591725

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

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