Recombinant Human FGF-basic/FGF2 145aa Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0005
Recombinant Human FGF-basic,145aa (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 16 kDa and purity greater than 97%.
Recombinant Human FGF-basic,145aa (2 ug/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band at 16 kDa and purity greater than 97%.

Recombinant Human FGF-basic/FGF2 145aa Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0005
/
Size

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Product Overview

Product Name Recombinant Human FGF-basic/FGF2 145aa Protein
Target Symbol FGF2
Alternative Names Fibroblast Growth Factor basic, BFGF, Basic Fibroblast Growth Factor, Heparin-Binding Growth Factor 2, HBGF2, FGFB, FGF2, Prostatropin
Accession Number NP_001997
Expression System E.Coli.
Molecular Weight 16.3 kDa (predicted)
Sequence Ala144-Ser288
Purity >97% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining.
Activity Determined by a cell proliferation assay using BALB/c 3T3 mouse fibroblast cells. The ED50 for this effect is typically <0.1 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.05 EU/1ug of the protein as determined by the LAL method.
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 ug/mL in sterile PBS.
Target Function Fibroblast Growth Factor Basic (FGF-basic), also known as Fibroblast Growth Factor 2 (FGF-2), displays a diverse range of roles in normal physiological processes, such as proliferation of fibroblasts, endothelial cells and stem cells leading to roles in tissue neovascularization and tissue repair. It promotes the survival and proliferation of neurons, as well as the growth of neuronal processes. FGF-basic has also been implicated in neuroprotection, helping to protect neurons from damage. During embryogenesis, FGF-basic is involved in various developmental processes, including cell differentiation, morphogenesis, and organogenesis. Although FGF-basic is primarily used in stem cell culture to maintain undifferentiated cells, modulation of growth factor supplements in media containing FGF-basic will promote differentiation. FGF-basic is a ligand for FGF Receptors 1-4 (CD331, CD332, CD333, CD334).
Tissue Specificity FGF-basic expression has been identified in endothelial tissue, epithelial tissue, mesenchymal tissue, neuronal tissue and various cancers.
Cellular Localization FGF-basic exists as multiple isoforms that are either initiated with the CUG codon (retained intracellularly) or the AUG codon (secreted forms differing by the removal of a 9aa prosegment).
Involvement In Disease FGF-basic is overexpressed in many types of cancer and is associated with tumor growth, angiogenesis, and metastasis.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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