Recombinant Human Ezrin Protein

Name: Recombinant Human Ezrin Protein
Brand: Beta LifeScience
SKU: BL-2075NP
Availability: InStock

BL-2075NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Ezrin is produced by our E.coli expression system and the target gene encoding Met1-Leu586 is expressed.
Accession	P15311
Synonym	Ezrin; Cytovillin; Villin-2; p81; EZR; VIL2
Gene Background	Ezrin is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. The N-terminus of ezrin contains a FERM domain which is further subdivided into three subdomains. The C-terminus contain a ERM domain. As a member of the ERM protein family, Ezrin serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. Ezrin probably involved in connections of major cytoskeletal structures to the plasma membrane. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal binds to actin, phosphatidylinositol bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2. In epithelial cells, Ezrin is required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, Ezrin is required for normal macropinocytosis.
Molecular Mass	69.4 KDa
Apmol Mass	80 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 10mM HEPES, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Subcellular Location	Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell projection, microvillus.
Database References	HGNC: 12691 OMIM: 123900 KEGG: hsa:7430 STRING: 9606.ENSP00000338934 UniGene: PMID: 29587669 Ezrin and myosin II play critical roles in enhancing line tension by promoting the formation of an actomyosin ring. PMID: 28643776 these findings suggest that baicalein inhibits the proliferation, migration and invasion and induces apoptosis in Osteosarcoma (OS) cells by activating the miR183/Ezrin pathway, revealing a novel mechanism underlying antiOS effects of baicalein. PMID: 29845278 Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion. PMID: 29259079 High Ezrin expression is associated with osteosarcoma. PMID: 29656060 L1CAM promotes esophageal squamous cell carcinoma tumorigenicity by upregulating ezrin expression. PMID: 28939985 This is the first study to verify the relationship of the expression of RhoA and Ezrin proteins in vaginal tissue of Postmenopausal atrophic vagina. PMID: 28843271 ezrin facilitates AQP2 endocytosis, thus linking the dynamic actin cytoskeleton network with AQP2 trafficking. PMID: 28754689 FUT4/LeY was critical to the TAMs-mediated EMT; this process might be associated with the up-regulation of Ezrin phosphorylation by FUT4/LeY-mediated fucosylation PMID: 28423676 CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family. PMID: 27793041 Data suggest that EGF induces colorectal cancer cells to undergo epithelial-mesenchymal transition, enhances their ability to invade/migrate, and promotes phosphorylation of Ezrin at Tyr353. (EGF = epidermal growth factor) PMID: 28535417 Binding of phosphatidylinositol 4,5-biphosphate to ezrin induces a conformational change permitting the insertion of the LOK C-terminal domain to wedge apart the membrane and F-actin-binding domains of ezrin. The N-terminal LOK kinase domain can then access a site 40 residues distal from the consensus sequence that collectively direct phosphorylation of the appropriate threonine residue. PMID: 28430576 The expression pattern and subcellular localization of ezrin and moesin correlate with clinicopathological variables such as patients' age, tumor grade and hormonal status. PMID: 28624994 Ezrin represents a promising target for the development of strategies aimed at preventing the progression of cervical cancer. PMID: 26933912 Ezrin S66 phosphorylation enhances filopodia formation, contributing to the regulation of invasion and metastasis of esophageal squamous cell carcinoma cells PMID: 28504189 The results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site. PMID: 28889652 Ezrin protein expression is a promising biomarker in estimating the outcome of stage II colorectal cancer patients. When combined with microsatellite status its ability in predicting disease outcome is further improved PMID: 28953975 Ezrin is down-regulated during cholangiocarcinogenesis, and its loss results in a more aggressive phenotype. PMID: 26791814 A signature of ezrin-interacting proteins accurately predicts esophageal squamous cell carcinoma patient survival or tumor recurrence. PMID: 28603065 The results of this meta-analysis suggest that ezrin positive immunoexpression confers a higher risk of recurrence and a worse survival in osteosarcoma patients. PMID: 23805177 PM blebbing triggered SRF-mediated up-regulation of the metastasis-associated ERM protein Ezrin. Notably, Ezrin is sufficient and important to sustain bleb dynamics for cell-in-cell invasion when SRF is suppressed. PMID: 28774893 EZR is a novel biomarker in terms of invasion among the 3 subtypes of NFPAs, and it is a promising guide for therapeutic decision making as well. PMID: 28093347 Increased ezrin and HER2 expression in patients with salivary gland carcinomas represents a high-grade histopathological subtype. PMID: 28300573 SMYD3 enhances tumorigenicity in esophageal squamous cell carcinoma by enhancing transcription of ezrin and LOXL2, which are involved in proliferation, migration, and invasion. PMID: 26980013 3-dimensional cell cultures were found to mimic different tumor sites and be applicable as a model. The in vitro results concur with the clinical specimen analysis, suggesting that in ovarian carcinoma, the role of ezrin in disease progression is more pronounced than that of p130Cas. PMID: 27622508 The expression of ezrin was up-regulated and significantly associated with the stage, lymph node involvement and distant metastasis PMID: 28261953 there were significant decreases in intercellular adhesion molecules 1 (ICAM1), ezrin (EZR), mitogen-activated protein kinase kinase 2 (MAP2K2), and nitric oxide synthase 3 (NOS3) gene expressions in metabolic syndrome patients. PMID: 26956845 Immunohistochemistry staining for ezrin, was similar in AFX and UPS tumors. PMID: 28079637 Ezrin and HER2/neu are overexpressed and coexpressed in osteosarcoma with adverse prognostic features such as high grade. Therefore, ezrin and HER2/neu could be potential prognostic markers and treatment targets for osteosarcoma PMID: 26067138 Study indicates the usual relationship between estrogen and ezrin induction is abridged. Study suggests that changes in ezrin may be associated with the development of the invasive phenotype and penetration of the basement membrane. PMID: 27688241 The present study showed over-expression of ezrin and moesin in colorectal carcinoma PMID: 27042764 study indicates that the presence of autoantibodies against Ezrin is significantly associated with ESCC PMID: 28298808 Ezrin protein is highly expressed in human PHC tissue which can be used for the prediction of metastasis disease. PMID: 28230040 results show that the activation of the ezrin-pAkt signaling axis is associated with the more aggressive clinicopathological features of PPA compared with LPA PMID: 27059464 Ezrin and p65 interactions in MDA-MB-231 cells were confirmed using co-immunoprecipitation. PMID: 27420986 the distribution of NHERF1 in ovarian cancer and reveals a different regulation of NHERF1 and EZRIN expression in ovarian tumors which represents the complexity of the molecular changes of this disease PMID: 27823775 Phosphorylation of ezrin together with its binding to phosphatidylinositol-4,5-bisphosphate tethers the F508del CFTR to the actin cytoskeleton, stabilizing it on the apical membrane and rescuing the sub-membrane compartmentalization of cAMP and activated PKA. PMID: 26823603 Data show that gene silence of ezrin inhibits the proliferation and invasion of prostate cancer PC-3 cells, meanwhile the level of E-cadherin is upregulated and N-cadherin is downregulated. PMID: 27371852 Knockdown of ezrin in HUVECs significantly induced the morphogenetic changes and cytoskeletal reorganization of the transfected cells, and also reduced cell migration and angiogenesis capacity in vitro. PMID: 27072970 High EZRIN expression is associated with prostate cancer. PMID: 26799186 Elevated Ezrin expression is associated with a poor prognosis in a variety of solid tumors. PMID: 26632332 These findings suggest that ezrin-EGFR interaction augments oncogenic functions of EGFR and that targeting ezrin may provide a potential novel approach to overcome erlotinib resistance in non-small cell lung cancer cells PMID: 26936397 The value of ezrin expression as a prognostic biomarker is further consolidated in urothelial cancer. PMID: 25278252 Suggest a role for ezrin in advanced glycation end product-induced podocyte damage. PMID: 26032400 Phospho-Ezrin/Radixin/Moesin (ERM) inhibit cell adhesion, and therefore, dephosphorylation of ERM proteins is essential for cell adhesion.Phospho-ERM induce formation and/or maintenance of spherical cell shape. PMID: 26555866 Activation of liver PKCs during cholestasis leads to Ezrin Thr567 phosphorylation resulting in MRP2 internalization and degradation where ubiquitin ligase E3 GP78 is involved. PMID: 26212029 Data indicate that a quinoline-based small molecule, NSC305787, directly binds to ezrin and inhibits its functions. PMID: 26358752 We identified and confirmed that Fra-1 affected the expression level of CTTN and EZR in vitro through LC-MS/MS analyses and western blot technology. PMID: 26330014 Data show that both Ezrin and SIX1 proteins are highly expressed in alpha fetoprotein-negative hepatocellular carcinoma (HCC) and significantly related with the TNM stage. PMID: 26927385 it was found that expression of miR-96 was negatively correlated with the metastatic ability of renal cell carcinoma, and that downregulation of miR-96 could suppress the invasion of renal cancer cell via downregulation of Ezrin expression. PMID: 26419932

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