Recombinant Human Excitatory Amino Acid Transporter 4 (SLC1A6) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-09471P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Excitatory Amino Acid Transporter 4 (SLC1A6) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-09471P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Excitatory Amino Acid Transporter 4 (SLC1A6) Protein (GST) is produced by our E.coli expression system. This is a protein fragment. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P48664 |
Target Symbol | SLC1A6 |
Synonyms | SLC1A6; EAAT4Excitatory amino acid transporter 4; Sodium-dependent glutamate/aspartate transporter; Solute carrier family 1 member 6 |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-GST |
Target Protein Sequence | MVTIIHPGKGSKEGLHREGRIETIPTADAFMDLIRNMFPPNLVEACFKQFKTQYSTRVVTRTMVRTENGSEPGASMPPPFSVENGTSFLENVTRALGTLQEMLSFEETVPVPGSANGINALGL |
Expression Range | 149-271aa |
Protein Length | Partial |
Mol. Weight | 40.4kDa |
Research Area | Neuroscience |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. |
Subcellular Location | Cell membrane; Multi-pass membrane protein. |
Protein Families | Dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family, SLC1A6 subfamily |
Database References | |
Tissue Specificity | Brain. Expressed densely and selectively in cell bodies of Purkinje cells. |
Gene Functions References
- Decreased SLC1A6 expression in neuregulin 1 risk variant may be an adaptive effect to restore glutamate signalling in schizophrenia patients. PMID: 22424243
- A twofold difference in functional EAAT4 levels is sufficient to alter signaling to Bergman glia in reporter mice. PMID: 22302796
- strate-dependent gating of anion channels associated with excitatory amino acid transporter 4. PMID: 21572047
- a conserved aspartate determines pore properties of anion channels associated with excitatory amino acid transporter 4 (EAAT4) PMID: 20519505
- At least one susceptibility locus for schizophrenia may be located within or nearby SLC1A6. PMID: 17221839
- Independent, rather than cooperative anion conductance gating significantly alters predictions of the influence that EAAT4-mediated anion currents will have on synaptic transmission at low glutamate concentrations. PMID: 17360917
- conclusion, maximal glutamate transport modulation by SGK1 is accomplished by direct EAAT4 stimulation and to a lesser extent by inhibition of intrinsic Nedd4-2. PMID: 17442044