Human ESR1 (Estrogen Receptor) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-04809P
SDS-PAGE analysis of Human ESR1 (Estrogen Receptor) - Recombinant Protein, CAT# BLT-04809P, showing >95% purity under 15% SDS-PAGE (Reduced)
Human ESR1 (Estrogen Receptor) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-04809P
Regular price
$59500
$595.00
Sale price$54500
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Quantity Pricing
| Pack Size | Price (USD) |
|---|---|
| 500 µg | $1,375 |
| 1 mg | $2,245 |
For direct online orders, quantity pricing will be displayed in cart when you add 5x100ug or 10x100ug
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Product Overview
| Product Name | Recombinant Human Estrogen Receptor Alpha (ERa/ESR1) Protein |
| Product Overview | This recombinant human Estrogen Receptor Alpha (ERa/ESR1) protein includes amino acids 178-438aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id | P03372 |
| Recommended Name | Estrogen receptor |
| Gene Name | ESR1 |
| Synonyms | Estrogen Receptor; ER; ER-Alpha; Estradiol Receptor; Nuclear Receptor Subfamily 3 Group A Member 1; |
| Species | Human |
| Predicted Molecular Mass | 33 kDa |
| Expression System | E.coli |
| Expression Range | 178-438aa |
| Tag | N-6His |
| Purity | >95% |
| Formulation | Lyophilized |
| Buffer | Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications | Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area | Transcription |
| Target Function | Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3.; Involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Binds to ERE and inhibits isoform 1. |
| Subcellular Location | [Isoform 1]: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.; Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated. |
| Protein Family | Nuclear hormone receptor family, NR3 subfamily |
| Associated Diseases | Estrogen resistance (ESTRR) |
| Tissue Specificity | Widely expressed. Not expressed in the pituitary gland.; [Isoform 3]: Widely expressed, however not expressed in the pituitary gland. |
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