Recombinant Human Endothelin B Receptor (EDNRB) Protein (His), Active

Name: Recombinant Human Endothelin B Receptor (EDNRB) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-06112P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/ml can bind human EDN1 with a linear range of 6.25-25.00 μg/ml.

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Product Overview

Description	Recombinant Human Endothelin B Receptor (EDNRB) Protein (His), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Activity	Measured by its binding ability in a functional ELISA. Immobilized EDNRB at 5 μg/ml can bind human EDN1 with a linear range of 6.25-25.00 μg/ml.
Uniprotkb	P24530
Target Symbol	EDNRB
Synonyms	EDNRB; ETRB; Endothelin receptor type B; ET-B; ET-BR; Endothelin receptor non-selective type
Species	Homo sapiens (Human)
Expression System	in vitro E.coli expression system
Tag	C-10His
Target Protein Sequence	EERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAEVPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILIASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIKGIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSFYFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLYNQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKSCLCCWCQSFEEKQSLEEKQSCLKFKANDHGYDNFRSSNKYSSS
Expression Range	27-442aa
Protein Length	Full Length of Mature Protein
Mol. Weight	50.3kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family, Endothelin receptor subfamily, EDNRB sub-subfamily
Database References	HGNC: 3180 OMIM: 131244 KEGG: hsa:1910 STRING: 9606.ENSP00000366416 UniGene: PMID: 28817788 the results suggest that aberrant expression of Gli1 serves a role in HSCR by targeting EDNRB. PMID: 29484400 Endothelin B receptor (ETBR) is overexpressed in glioblastoma and may be a prognostic marker and useful target for treating cancer patients. PMID: 29409474 our current findings suggest that human neutrophils display functional ETB receptors with calcium signaling capability, leading to accentuated adhesion to the endothelium. PMID: 28385784 ETBR function contributes to diminished endothelium-dependent dilation in previously preeclamptic women. PMID: 29042489 An IL2-targeted antibody failed to detect transfected ETB in HEK293 cultures. In contrast, the NT-targeted antibody accurately detected transfected ETB in HEK293 cultures by labeling a 37-kDa band. PMID: 25232999 This study concluded that ETB receptors mediate vasodilation in young women, but this effect is lost after menopause. PMID: 28438762 Our results suggest that EDNRB rs5351 single nucleotide polymorphism is a strong independent predictor of essential hypertension in men of Tatar ethnic origin. PMID: 28350619 No rare EDNRB variants were identified among 57 patients with Hirschsprung disease. PMID: 26395553 Taking into account that the cohort we screened was deprived of patients previously found mutated in MITF (about 15%) and SOX10 (another 15%), our 8% mutation rate indicates that EDNRB heterozygous mutations represent about 5%-6% of all WS2, making it the third gene of this subtype. PMID: 28236341 The high level of ETBR expression observed in CMS patients compared to healthy controls suggests that the cerebral hypoxia diastolic reaction is possible due to ETBR, rather than ET-1 itself. PMID: 27485004 crystal structures of human endothelin type B receptor in the ligand-free form and in complex with the endogenous agonist endothelin-1 PMID: 27595334 Wnt-dependent EdnrB signaling can rescue the defects in melanocyte regeneration caused by Mc1R loss. PMID: 27134165 This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies PMID: 27193723 Serum EDNRB hypermethylation is a highly specific but not sensitive serum biomarker for HNSCC PMID: 24995714 High Endothelin B receptor expression is associated with malignant gliomas. PMID: 26645886 EDNRB plays a key role in hypoxia tolerance PMID: 26240367 The increased gene expression of MITF and EDNRB in UVB-exposed NHMs. PMID: 26030901 culturing of reconstructed skin with microvascular endothelial cells led to increased skin pigmentation that could be prevented by inhibiting EDNRB PMID: 26308584 Polymorphisms of the EDNRB gene may influence the susceptibility to obesity and can interact with plasma arsenic levels PMID: 25799405 Cumulative effects of the RET and EDNRB genes contribute to long-segment and total colonic aganglionosis. PMID: 25638620 Endothelin receptor B expression was significantly lower while higher staining grade was found in basal cell carcinoma rather than squamous cell carcinoma or psoriasis. PMID: 25946671 Its expression by lymphoma and endothelial cells may mediate trafficking of tumor-infiltrated lymphocytes, which may explain the immune escape processes of primary central nervous system lymphomas. PMID: 25433721 The results show that MitA can effectively be used to block ETBR mediated vasoconstriction as a supplement to an existing ischemic stroke therapy. PMID: 25479176 Identification of heterozygous deletion of the entire EDNRB in a father and 2 children. Since heterozygous deletion was the only change identified in EDNRB, the family represents one of the few cases of autosomal dominant inheritance of Waardenburg type 4 PMID: 25118007 The anti-EDNRB ADC combines well with BRAF and MEK inhibitors and could have therapeutic use in the majority of human melanoma cases. PMID: 24651527 A novel stop mutation in the EDNRB gene was found in a family with Hirschsprung's disease associated with multiple sclerosis. PMID: 24726125 No biologically significant correlation was observed in the ETBR immunohistochemistry of human pancreatic ductal adenocarcinoma sections PMID: 24549269 There was no difference in EDNRB mRNA expression between reflux esophagitis, Barrett's esophagus, and controls. PMID: 23384184 Hypermethylation of EDNRB promoter contributes to the risk of colorectal cancer. PMID: 24326135 The endothelin B receptor was demonstrated to mediate the proliferation of oligodendroglioma cells according to an ERK-dependent mechanism. PMID: 24145738 The -615 to -624 bp region is the key binding site of AP-1 in ETB receptor promoter and was found to be crucial for inducible ETB receptor expression. PMID: 24265756 The levels of the EDNRB, HJURP and p60/CAF-1 proteins were strongly associated with overall survival in high-grade gliomas patients (p<0.001, p<0.001 and p=0.002, respectively), whereas the one of PDLI4 was not (P=0.11). PMID: 24039914 Gene expression profiling for uveal melanoma may be obtained from patients who were previously treated with radiotherapy PMID: 24511587 Endothelin receptor type B expression was correlated with several clinicopathological factors including tumor differentiation, tumor depth, and lymph node metastasis. PMID: 24357795 EDNRB is generally accepted as a major gene for Hirschsprung's disease. PMID: 23579558 Endothelin receptor type B is a novel pathway and therapeutic target for enhancing remyelination in multiple sclerosis. PMID: 23518706 Expression of endothelin A and B receptors and endothelin-1 in the pulmonary arteries is significantly increased in failed Fontan patients. PMID: 21356562 Data indicate that endothelin receptor B (EDNRB) overexpression facilitates intracranial melanoma growth. PMID: 22865454 Our findings demonstrate that aldosterone modulates an ET(B) cysteinyl thiol redox switch to decrease pulmonary endothelium-derived NO(.) and promote pulmonary arterial hypertension. PMID: 22787113 Loss of EDNRB is associated with metastasis in clear-cell renal cell carcinoma. PMID: 22213152 results obtained give evidence of a possible participation of receptor for endothelin B in the pathogenesis of the itch in the dermatitis herpetiformis PMID: 22535604 promoter hypermethylation of EDNRB gene is highly prevalent in gastric cancer PMID: 21264540 In transgenic Ednrb-deficient mice, sacral crest-derived cells diminish in number from fetal to postnatal stages. PMID: 21858821 Survival analysis shows that the ETB receptor high expression group had significantly shorter disease-free survival and overall survival rates than the low expression group. PMID: 21770857 Attenuated ET-B receptor mediated vasodilatation during local skin warming compared to Controls. PMID: 21825025 3 novel mutations in EDNRB gene associated with Waardenburg-Shah syndrome (WS4) in Pakistani patients. PMID: 21547364 No association of S305N EDNRB polymorphism with sporadic melanoma risk in the French or Italian populations, but there was an indication that EDNRB might be a melanoma-predisposing gene in French patients with a hereditary predisposition to melanoma. PMID: 21507037 Endothelin type B receptor-mediated contractions of the cerebrovasculature are significantly reduced following inhibition of B-raf kinase. PMID: 21223556 cigarette smoke extract induced ET(B) receptor overexpression by a feed forward mechanism mediated partly by ET release, promoting pulmonary artery endothelial cell dysfunction and attenuated by ET(B) receptor blockade, Rho kinase and ROS inhibition PMID: 20698855

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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