Recombinant Human Dual Specificity Protein Phosphatase 6 (DUSP6) Protein (His&Myc)

Name: Recombinant Human Dual Specificity Protein Phosphatase 6 (DUSP6) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-07859P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Dual Specificity Protein Phosphatase 6 (DUSP6) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q16828
Target Symbol	DUSP6
Synonyms	Dual specificity protein phosphatase PYST1 (Mitogen-activated protein kinase phosphatase 3) (MAP kinase phosphatase 3) (MKP-3)
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MIDTLRPVPFASEMAISKTVAWLNEQLELGNERLLLMDCRPQELYESSHIESAINVAIPGIMLRRLQKGNLPVRALFTRGEDRDRFTRRCGTDTVVLYDESSSDWNENTGGESVLGLLLKKLKDEGCRAFYLEGGFSKFQAEFSLHCETNLDGSCSSSSPPLPVLGLGGLRISSDSSSDIESDLDRDPNSATDSDGSPLSNSQPSFPVEILPFLYLGCAKDSTNLDVLEEFGIKYILNVTPNLPNLFENAGEFKYKQIPISDHWSQNLSQFFPEAISFIDEARGKNCGVLVHCLAGISRSVTVTVAYLMQKLNLSMNDAYDIVKMKKSNISPNFNFMGQLLDFERTLGLSSPCDNRVPAQQLYFTTPSNQNVYQVDSLQST
Expression Range	1-381aa
Protein Length	Full Length
Mol. Weight	49.8 kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors.
Subcellular Location	Cytoplasm.
Protein Families	Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Database References	HGNC: 3072 OMIM: 602748 KEGG: hsa:1848 STRING: 9606.ENSP00000279488 UniGene: PMID: 29578153 DUSP6 expression in skeletal muscle was reduced by 43% just after exercise and remained below pre-exercise level after 2 h recovery. PMID: 28989118 Data show that oxidative stress and MAP kinase phosphatase 3 (MKP3) inhibition play a critical role in procyanidin B2 3,3''-di-O-gallate (B2G2)-induced cell death in prostate cancer (PCa) cells through sustained activation of both ERK1/2 and AMPKalpha. PMID: 28876465 High DUSP6 expression is associated with Lung Squamous Cell Carcinoma. PMID: 27613525 Authors demonstrate the broad applicability of this recombination-based method and they proved its potential to identify new drug targets via the identification of the tumor suppressor DUSP6 as potential synthetic lethal target in melanoma cell lines with BRAF V600E mutations and high DUSP6 expression. PMID: 28423600 PICSAR has a role in promoting cSCC progression via activation of extracellular signal-regulated kinase 1/2 signaling pathway by downregulating DUSP6 expression PMID: 27049681 DUSP6 rs2279574 SNPs was not associated with chemoradiotherapy response, whereas tumor regression, weight loss, clinical stage, and cigarette smoking were independent prognostic predictors for these Chinese patients with non-small cell lung cancer. PMID: 28231233 DNA samples from each patient were genotyped for DUSP6 and TOP2A SNPs PMID: 27156317 Suppression of the FOXA1/DUSP6 signaling pathway may contribute to the development of Hirschsprung disease. PMID: 26097584 These observations led us to conclude that increased TSH signaling overcomes OIS and is essential for B-RafV600E-induced papillary thyroid carcinogenesis. PMID: 25499223 Dusp6 expression was higher in progestin-sensitive atypical endometrial hyperplasia groups compared with progestin-resistant groups. After treatment, Dusp6 expression was upregulated in progestin-sensitive groups, but not in progestin-resistant groups. PMID: 25451692 Our results suggest that DUSP6 gene expression in tumour samples may be a prognostic marker in NSCLC PMID: 25344212 DUSP6 downregulated the expression of matrix metallopeptidase 3. PMID: 25241655 These results therefore indicate that p53-mediated up-regulation of MKP-3 contributes to the establishment of the senescent cellular phenotype through dephosphorylating ERK1/2 PMID: 25414256 Downregulation of Dusp6, Sprouty4, and Sef--negative modulators of FGF2/ERK1/2 signaling--was present in eutopic endometria of adenomyosis, which may play critical roles in the development of adenomyosis. PMID: 24681741 Higher expression of DUSP6 in tumor tissue, than in peritumor tissue, is associated with the recurrence after curative resection of HCC, and the relative tumor DUSP6 expression has good power to predict the recurrence of HCC. PMID: 24709168 suggest an important role for DeltaNp63alpha in preventing cancer metastasis by inhibition of Erk2 signaling via MKP3 subscribing PMID: 23246965 Dusp6 overexpression is involved in the pathogenesis and development of human endometrial adenocarcinoma. PMID: 23419500 Increased DUSP6 expression is associated with papillary thyroid carcinoma. PMID: 24222120 Depletion of DUSP6 reduced the viability of cancer cell lines and increased the cytotoxicity of EGFR and other targeted inhibitors, and cytotoxic agents. PMID: 23839489 missense mutation, c.545C>T (p.Ser182Phe), in the DUSP6 gene may be genetically linked to the Class III malocclusion PMID: 23965468 DUSP6 is overexpressed in papillary and poorly differentiated thyroid carcinoma and contributes to neoplastic properties of thyroid cancer cells. PMID: 23132790 The results provide insights on the modulatory role of p53 in the survival pathway by up-regulating DUSP6. PMID: 23108049 study reports that naive CD4 T cells from elderly individuals have reduced signaling capacity of the ERK pathway; the underlying mechanism is an age-related decline in miR-181a expression and associated rise in levels of DUSP6 expression PMID: 23023500 ischemia/reperfusion inhibited eNOS expression by inactivation of ERK1/2, leading to decreased NO formation through a MKP-3-dependent mechanism in endothelial cells PMID: 22848708 DUSP6 plays an important role in the pathogenesis of bipolar disorder, particularly in women. PMID: 22155192 MESP2, HES7 and DUSP6 genes may not be involved in the etiopathogenesis of sporadic and non-syndromic CS in Chinese Han population. PMID: 22744456 A link between DUSP6 expression and high-risk features of papillary thyroid carcinoma suggested that DUSP6 is an important independent factor affecting the signaling pathways in established papillary thyroid carcinoma. PMID: 22535643 DUSP6 is important in melanoma and it plays a different role in the distinct subtype of mouse melanoma compared with that in classic human melanoma. PMID: 22171919 caspase-3 cleavage of MKP3 down-regulates MKP3 full length and renders active MKP3 fragments, which may participate in novel regulatory pathways controlling the subcellular localization and activation of ERK1/2 during apoptosis. PMID: 22504224 Studies identified a single gene DUSP6, whose expression was associated with sensitivity to GSK1120212. PMID: 22169769 Studies indicate that ERKs stimulate their own dephosphorylation by directly binding to phosphatases, such as MKP-3, and activating them. PMID: 22028468 Loss of DUSP6 is associated with esophageal squamous cell carcinoma and nasopharyngeal carcinoma. PMID: 21387288 DUSP6 plays an important role in cancer resistance in different subtypes of non-small cell lung carcinoma. PMID: 21680106 upregulation of DUSP6 exerts a tumor-promoting role in human glioblastomas exacerbating the malignant phenotype. PMID: 21499306 MKP3 not only controls the activities of ERK2 and p38alpha but also mediates cross-talk between these two MAPK pathways. PMID: 21454500 This study shows that post-transcriptional regulation is a key process in the control of DUSP6 expression. PMID: 20665674 DUSP6 methylation is a rare event in endometrial cancer. Silencing of the DUSP6 phosphatase is unlikely to contribute to constitutive activation of the ERK kinase cascade in endometrial cancer. PMID: 20638106 Dual specificity phosphatase 6 (DUSP6) is an ETS-regulated negative feedback mediator of oncogenic ERK signaling in lung cancer cells. PMID: 20097731 Angiotensin II-induced upregulation of MAP kinase phosphatase-3 mRNA levels mediates endothelial cell apoptosis PMID: 11998972 Results show that DUSP6 exerts apparent tumor-suppressive effects in vitro and suggest that DUSP6 is a strong candidate tumor suppressor gene at 12q22 locus. PMID: 12759238 Constitutive induction of p-Erk1/2 accompanied by reduced activities of protein phosphatases 1 and 2A and MKP3 due to reactive oxygen species during cellular senescence. PMID: 12840032 Study gives first direct evidence that the N-terminal domain of MKP3 mediates intramolecular dephosphorylation between the monomeric form of phosphorylated extracellular signal-regulated kinase and a monomer of MKP3. PMID: 14690430 cytoplasmic localization of MKP3 is mediated by a chromosome region maintenance-1 (CRM1)-dependent nuclear export pathway; the ability of MKP3 to cause the cytoplasmic retention of ERK2 requires both a functional kinase interaction motif and NES PMID: 15269220 Hypermethylation with modification of histone deacetylation play an important role in transcriptional suppression of DUSP6 in human pancreatic cancer PMID: 15824892 Results suggest that the abrogation of DUSP6 is associated exclusively with progression from pancreatic intraepithelial neoplasia to the invasive ductal carcinoma. PMID: 15832194 Results demonstrate that in the Korean population DUSP6 may contribute to the etiology of bipolar disorder, but not schizophrenia. PMID: 16491131 Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113 even upon over-expression DUSP6 fails to inactivate ERK5, confirming that it is indeed an ERK1/2-specific DUSP PMID: 18280112 MKP3 can act to enhance DAT function and is a phosphatase involved in regulating dynamin-dependent endocysis PMID: 18434601

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human Dual Specificity Protein Phosphatase 6 (DUSP6) Protein (His&Myc)

Recombinant Human Dual Specificity Protein Phosphatase 6 (DUSP6) Protein (His&Myc)

Product Overview

Target Details

FAQs