Recombinant Human DNTT/TDT Protein (N-6His)
Beta LifeScience
SKU/CAT #: BL-2609NP

BL-2609NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Recombinant Human DNTT/TDT Protein (N-6His)
Beta LifeScience
SKU/CAT #: BL-2609NP
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human DNA Nucleotidylexotransferase is produced by our E.coli expression system and the target gene encoding Met1-Ala509 is expressed with a 6His tag at the N-terminus. |
Accession | P04053 |
Synonym | DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltr; Terminal deoxynucleotidyltransferase; Terminal transferase; DNTT; TDT |
Gene Background | Terminal deoxynucleotidyl transferase (TdT) is a highly conserved vertebrate enzyme that possesses the unique ability to catalyze the random addition of deoxynucleoside 5'-triphosphates onto the 3'-hydroxyl group of a single-stranded DNA. It plays an important role in the generation of immunoglobin and T-cell receptor diversity. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
Molecular Mass | 60.7 KDa |
Apmol Mass | 62 KDa, reducing conditions |
Formulation | Supplied as a 0.2 μm filtered solution of 0.1M KH2PO4, 200mM KCl, 1mM 2-Mercaptoethanol, 50% Glycerol, pH 7.2. |
Endotoxin | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Biological Activity | Not tested |
Reconstitution | |
Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
Shipping | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Usage | For Research Use Only |
Target Details
Target Function | Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells. |
Subcellular Location | Nucleus. |
Protein Families | DNA polymerase type-X family |
Database References |
Gene Functions References
- TdT protein can be found in cells of epithelial origin and specifically sebaceous cells, both benign and malignant. PMID: 28677299
- Structural basis for a new templated activity by terminal deoxynucleotidyl transferase and implications for V(D)J recombination have been described. PMID: 27499438
- TdT-positive cells may be part of the inflammatory milieu in infant kidneys. PMID: 28248816
- Absence of TdT expression identifies a subset of high-risk T-acute lymphoblastic leukemia/lymphoma that overlaps with, but is not identical to, the ETP leukemia, providing additional prognostic value. PMID: 23702731
- Our study confirms that PAX5 and TdT expression can be expressed in a high percentage of Merkel cell carcinomas and so when positive are not diagnostic of lymphoblastic leukemia/lymphoma. PMID: 23329999
- role in DNA repair PMID: 11974916
- Overexpression of newly discovered alternatively spliced short or long human TdT isoforms greatly reduces the efficiency of recombination, which is reverted to normal levels by the simultaneous expression of both enzymes. PMID: 15356150
- In spleen, appendix and branchial cleft cysts the range of TdT-positivity was 0-13, 0-96 and 0-6 TdT+ cells per high-power field. PMID: 16885057
- The TdT binding, DNA binding and dimerization regions, and nuclear localization signal (NLS) in TdIF1, were identified. PMID: 17663723