Recombinant Human Dnaj Homolog Subfamily B Member 8 (DNAJB8) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09895P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Dnaj Homolog Subfamily B Member 8 (DNAJB8) Protein (His-SUMO)
Beta LifeScience
SKU/CAT #: BLC-09895P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Dnaj Homolog Subfamily B Member 8 (DNAJB8) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | Q8NHS0 |
Target Symbol | DNAJB8 |
Synonyms | DJ6; DnaJ (Hsp40) homolog; subfamily B; member 8; DnaJ homolog subfamily B member 8; Dnajb8; DNJB8_HUMAN; mDj6; MGC33884; OTTHUMP00000216243 |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-6His-SUMO |
Target Protein Sequence | MANYYEVLGVQASASPEDIKKAYRKLALRWHPDKNPDNKEEAEKKFKLVSEAYEVLSDSKKRSLYDRAGCDSWRAGGGASTPYHSPFDTGYTFRNPEDIFREFFGGLDPFSFEFWDSPFNSDRGGRGHGLRGAFSAGFGEFPAFMEAFSSFNMLGCSGGSHTTFSSTSFGGSSSGSSGFKSVMSSTEMINGHKVTTKRIVENGQERVEVEEDGQLKSVTVNGKEQLKWMDSK |
Expression Range | 1-232aa |
Protein Length | Full Length |
Mol. Weight | 41.7kDa |
Research Area | Signal Transduction |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Efficient suppressor of aggregation and toxicity of disease-associated polyglutamine proteins. |
Database References |
Gene Functions References
- DNAJB8 is a central and versatile player in the protein aggregation and degradation system.DNAJB8 protein keeps Parkin C289G mutant protein in a soluble, degradation-competent form. PMID: 27713507
- Results show that DNAJB8 expression is induced by heat shock stress which increases side population cells and cancer stem-like cells. PMID: 29316077
- Taken together, the results indicate that DNAJB8 is expressed and has role in CRC CSC/CIC and that DNAJB8 is a novel target of CRC CSC/CIC-targeting immunotherapy. PMID: 24450541
- The mechanism of DNAJB6 and DNAJB8 is suppression of polyQ protein aggregation by directly binding the polyQ tract. PMID: 23612975
- Cancer stem-like cell (CSC) maintenance and that it offers a candidate for CSC-targeting immunotherapy in renal cell carcinoma PMID: 22552285
- DNAJB6b and DNAJB8 are superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. PMID: 20159555