Recombinant Human Dna Repair Protein Xrcc3 (XRCC3) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-10120P
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SDS-PAGE.

Recombinant Human Dna Repair Protein Xrcc3 (XRCC3) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-10120P
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Product Overview

Description Recombinant Human Dna Repair Protein Xrcc3 (XRCC3) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb O43542
Target Symbol XRCC3
Synonyms CMM6; DNA repair protein XRCC3; RAD51 like; X ray repair complementing defective repair in Chinese hamster; X ray repair complementing defective repair in Chinese hamster cells 3; X ray repair cross complementing protein 3; X-ray repair cross-complementing protein 3; XRCC 3; XRCC3; XRCC3_HUMAN
Species Homo sapiens (Human)
Expression System E.coli
Tag N-6His-SUMO
Target Protein Sequence MDLDLLDLNPRIIAAIKKAKLKSVKEVLHFSGPDLKRLTNLSSPEVWHLLRTASLHLRGSSILTALQLHQQKERFPTQHQRLSLGCPVLDALLRGGLPLDGITELAGRSSAGKTQLALQLCLAVQFPRQHGGLEAGAVYICTEDAFPHKRLQQLMAQQPRLRTDVPGELLQKLRFGSQIFIEHVADVDTLLECVNKKVPVLLSRGMARLVVIDSVAAPFRCEFDSQASAPRARHLQSLGATLRELSSAFQSPVLCINQVTEAMEEQGAAHGPLGFWDERVSPALGITWANQLLVRLLADRLREEEAALGCPARTLRVLSAPHLPPSSCSYTISAEGVRGTPGTQSH
Expression Range 1-346aa
Protein Length Full Length
Mol. Weight 50.8kDa
Research Area Epigenetics And Nuclear Signaling
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
Subcellular Location Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion. Note=Accumulates in discrete nuclear foci prior to DNA damage, and these foci persist throughout the time course of DNA repair.
Protein Families RecA family, RAD51 subfamily
Database References
Associated Diseases Breast cancer (BC); Melanoma, cutaneous malignant 6 (CMM6)

Gene Functions References

  1. Although XRCC3 plays a role in asthma etiology, the variant XRCC3 genotypes do not serve as practicable predictive markers for asthma risk in Taiwanese. PMID: 30150425
  2. Our results indicated a link between ERCC1 rs3212986 and the onset of late gastrointestinal toxicity ..No association was found regarding the XRCC3 rs861539 polymorphism and any clinical toxicity event PMID: 28708208
  3. XRCC3 gene SNPs could influence the tumour aggressiveness expressed by tumour grade in hepatocellular carcinoma. PMID: 29895130
  4. XRCC3 deleterious variants were identified in breast and ovarian cancer cases. PMID: 29255180
  5. hypermethylation of homologous recombination DNA repair genes including RAD51B and XRCC3 is associated with an inflamed phenotype in squamous cell cancers of the head and neck, lung and cervix. PMID: 27683114
  6. The combined AA/AA genotype and GG/GA genotype frequencies for rs1799794 and rs709399 polymorphisms were significantly higher in thyroid cancer patients compared to control group when compared with combined AA/GG genotype as reference. PMID: 28490032
  7. based on the evidence from the large-scale case-control study and meta-analysis, the rs861539 within XRCC3 gene was associated with the risk of NPC under recessive model. PMID: 27639382
  8. our study suggested that the polymorphism of rs861539 in XRCC3 was associated with increased risk of PTC. PMID: 26264616
  9. XRCC3-Met allele seems to contribute to the development of OSCC, metastases and more advanced stages in these lesions. PMID: 26742000
  10. This meta-analysis suggested that the XRCC3 rs861539 polymorphism was not a risk factor for cutaneous melanoma susceptibility. [meta-analysis] PMID: 26922354
  11. XPD Lys751Gln polymorphism may be a risk factor for CCRCC. Regarding the XRCC3 Thr241Met polymorphism, an association with CCRCC was found only in XRCC3 Thr241Met/XPD Lys751Gln combined genotypes. PMID: 26682510
  12. XRCC3 T241M polymorphism may constitute a risk factor for hepatocellular carcinoma in the Chinese population (Meta-Analysis) PMID: 26662391
  13. the results obtained during this study suggest that rs1799794 in XRCC3 shows strong association with breast cancer development in Saudi females. PMID: 26881229
  14. XRCC3 Thr241Met polymorphism is associated with an increased risk of thyroid cancer in the overall population PMID: 26687776
  15. XRCC3 rs861539 TT is a potential predictive marker for triple-negative breast cancer in Taiwanese women PMID: 26543082
  16. our study firstly reports that XRCC3 Thr241Met gene polymorphism is associated with an elavated risk of osteosarcoma. PMID: 26662447
  17. XRCC3 Thr241Met gene polymorphism was associated with increased risk of osteosarcoma PMID: 26617908
  18. Our findings demonstrated that the T241M polymorphism in the XRCC3 gene may not be a risk factor for colorectal cancer development. PMID: 26600544
  19. The rs861539 and rs3212112 polymorphisms in the XRCC3 gene may influence the risk of glioma development in a Chinese population. PMID: 26400288
  20. XRCC3 Thr241Met polymorphism might be associated with breast cancer risk, especially in Asian populations and in patients without family history of breast ca [meta-analysis] PMID: 26498491
  21. results indicate that Thr241Met polymorphism was not associated with the development of laryngeal cancer in Turkish population. PMID: 25510985
  22. XRCC3 may be a novel radiosensitivity predictor and promising therapeutic target for esophageal squamous cell carcinoma PMID: 26383967
  23. XRCC3 deregulation is extensively involved in the chemoresistance mechanisms in rectal cancer patients. PMID: 26023803
  24. The T allele (CT and TT genotypes) of XRCC3 rs861539 contributes to increased risk of leiomyoma among Taiwanese women and may serve as a early detection and predictive marker. PMID: 26254358
  25. Association between XRCC3 polymorphisms and the risk of radiation-induced normal tissue adverse effects. [meta-analysis] PMID: 26091483
  26. XRCC3 gene rs861539 polymorphism was associated with the risk for gastric cancer in Asian populations. PMID: 25973083
  27. The XRCC3 genotype and allele frequencies were not significantly different between gastric cancer patients and controls in a North India population. PMID: 25642794
  28. XRCC3 Thr241Met polymorphism was associated with increased risk of colorectal cancer. PMID: 25824748
  29. XRCC3 gene polymorphism is not correlated with the radiotherapy-based multimodality treatment in rectal cancer. PMID: 25684513
  30. the XRCC3 Thr241Met genetic variant is associated with prostate cancer risk. PMID: 25834539
  31. CT and TT genotypes of XRCC3 rs861539 were associated with increased lung cancer risk. PMID: 26124335
  32. The frequency of heterozygous and homozygous genotype of Thr241Met polymorphism of XRCC3 were significantly higher in Pakistani breast cancer patients. PMID: 25556451
  33. The XRCC3 gene polymorphism was not found to be associated with the risk of oral cancer. [Meta-Analysis] PMID: 25556470
  34. The Thr241Met polymorphism of XRCC3 was associated with a 16 fold increase in head and neck cancer risk. PMID: 25556492
  35. This study displayed a significantly elevated risk for colorectal cancer in individuals with XRCC3 Thr/Met and Met/Met Genotype of about 2.5 times that with the Thr/Thr wild genotype. PMID: 25520078
  36. XRCC3 T241M polymorphism may not be a risk factor for lung cancer. PMID: 25501160
  37. The T allele of XRCC3 rs861539, interacts with smoking habit in increasing nasopharyngeal carcinoma(NPC) risk, may be an early detection marker for NPC. PMID: 25687493
  38. This meta-analysis provides evidence that the RAD51 and XRCC3 polymorphisms are not associated with an increased risk of AML in the total population. PMID: 23978154
  39. the aim of this study was to evaluate the clinical response to platinum-based chemotherapy and treatment outcome of gastric cancer patients in the present of ERCC1, ERCC2, NBN, RAD51, and XRCC3 gene polymorphisms. PMID: 25542228
  40. Association of DNA repair genes XRCC1 (Arg399Gln), (Arg194Trp) and XRCC3 (Thr241Met) polymorphisms with the risk of breast cancer: a case-control study in Egypt. PMID: 25340946
  41. XRCC3 Thr241Met may act as a favorable prognostic indicator for lung squamous cell carcinoma patients. PMID: 23443124
  42. This meta-analysis suggests that the T241M polymorphism of the XRCC3 gene confers a weakly increased breast cancer risk. PMID: 25169497
  43. There is no clear evidence showing a significant correlation between XRCC3 Thr241Met polymorphism and lung cancer risk in total population and stratified analysis by ethnicity. PMID: 25794597
  44. our results did not confirm the hypothesis of a possible role of NBN and XRCC3 SNPs in acute lymphoblastic leukaemia risk. PMID: 25176580
  45. there was no association between XRCC3 Thr241Met (rs861539) polymorphism and leukemia risk in the overall population, but significant association between XRCC3 Thr241Met (rs861539) polymorphism and leukemia risk was found in the Asian population. PMID: 24304418
  46. Studied the relation between XRCC3 Thr241Met polymorphism and lung cancer susceptibility of populations in East Asia. PMID: 25066399
  47. findings have suggested that APE1, XRCC3, XPD, and hOGG1 gene variants could facilitate the development of migraine disease. PMID: 24892639
  48. XRCC3 were associated with ovarian cancer risk overall for Caucasians. PMID: 25006581
  49. XRCC3 single nucleotide polymorphism and gene frequency in Russians, Tatars and Bashkirs. PMID: 25474887
  50. present meta-analysis demonstrates that XRCC3 C18067T polymorphism was not associated with risk of cutaneous melanoma but contributed a decreased risk to both basal cell carcinoma and squamous cell carcinoma PMID: 24454720

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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