Recombinant Human Dna Repair Protein Rad51 Homolog 4 (RAD51D) Protein (GST)

Name: Recombinant Human Dna Repair Protein Rad51 Homolog 4 (RAD51D) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-09135P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Dna Repair Protein Rad51 Homolog 4 (RAD51D) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O75771
Target Symbol	RAD51D
Synonyms	BROVCA4; DNA repair protein RAD51 homolog 4; HsTRAD; OTTHUMP00000163851; OTTHUMP00000163852; OTTHUMP00000163853; R51H3; RA51D_HUMAN; RAD51 homolog D (S. cerevisiae); RAD51 homolog D; RAD51 like 3 (S. cerevisiae); RAD51 paralog D; RAD51, S. cerevisiae, homolog of, D; RAD51-like protein 3; Rad51l3; Recombination repair protein; S. cerevisiae RAD51-like 3; TRAD
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MGVLRVGLCPGLTEEMIQLLRSHRIKTVVDLVSADLEEVAQKCGLSYKALVALRRVLLAQFSAFPVNGADLYEELKTSTAILSTGIGSLDKLLDAGLYTGEVTEIVGGPGSGKTQVCLCMAANVAHGLQQNVLYVDSNGGLTASRLLQLLQAKTQDEEEQAEALRRIQVVHAFDIFQMLDVLQELRGTVAQQVTGSSGTVKVVVVDSVTAVVSPLLGGQQREGLALMMQLARELKTLARDLGMAVVVTNHITRDRDSGRLKPALGRSWSFVPSTRILLDTIEGAGASGGRRMACLAKSSRQPTGFQEMVDIGTWGTSEQSATLQGDQT
Expression Range	1-328aa
Protein Length	Full Length
Mol. Weight	62.0kDa
Research Area	Epigenetics And Nuclear Signaling
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Subcellular Location	Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, telomere.
Protein Families	RecA family, RAD51 subfamily
Database References	HGNC: 9823 OMIM: 602954 KEGG: hsa:5892 STRING: 9606.ENSP00000466834 UniGene: PMID: 30111881 Results highlight the importance of a functional RAD51D-XRCC2 interaction to promote HR and prevent the development of HGSC. PMID: 28646019 variants in RAD51D were associated with moderate risks of breast cancer. PMID: 28418444 of Rad51d mediated by E3 Ligase Rnf138 has a role in the homologous recombination repair pathway PMID: 27195665 endogenous regulation of RAD51D by miR-103/107 was observed in several tumor subtypes; both miR-103 and miR-107 directly target and regulate RAD51 and RAD51D PMID: 24088786 We aimed to determine the prevalence of germline RAD51D mutations in Spanish breast and ovarian cancer families negative for BRCA1/BRCA2 PMID: 24130102 Our data provide additional evidence that RAD51D mutations are enriched among ovarian cancer patients, but are extremely rare among familial breast cancer patients. PMID: 23372765 loss-of-function mutations in RAD51D predispose to ovarian carcinoma but not to breast carcinoma. PMID: 22986143 RAD51D is primarily a moderate penetrance susceptibility gene for ovarian cancer, with clinical significance for the carriers. PMID: 22652533 The RAD51D should be included in genetic screening of ovarian cancer families that do not have BRCA1/BRCA2 mutations. PMID: 22415235 These data indicate that RAD51D mutation testing may have clinical utility in individuals with ovarian cancer and their families. PMID: 21822267 The N-terminal domain of Rad51D is required for the ssDNA-specific binding function of human Rad51D. PMID: 21111057 Polymorphisms in RAD51D gene is associated with breast cancer. PMID: 20054644 Homologous pairing and ring and filament structure formation activities of the human Xrcc2*Rad51D complex PMID: 11834724 RAD51L3 cooperates with Bloom Syndrome Protein during the late stages of homologous recombination processes that serve to restore productive DNA replication at sites of damaged or stalled replication forks PMID: 12975363 Telomere maintenance requires RAD51D. PMID: 15109494 E233G single nucleotide polymorphism is a low-penetrance susceptibility gene in the specific subgroup of high-risk familial breast cancer cases that are not related to BRCA1/2. PMID: 15170666 Interactions between RAD51D and its XRCC2 and RAD51C partners require a functional RAD51D Walker B ATPase motif, but not motif A. PMID: 16717288 The RAD51D E233G variant is not associated with breast cancer. PMID: 18058226

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.