Recombinant Human Dcc-Interacting Protein 13-Alpha (APPL1) Protein (His)

Name: Recombinant Human Dcc-Interacting Protein 13-Alpha (APPL1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-11042P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Dcc-Interacting Protein 13-Alpha (APPL1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9UKG1
Target Symbol	APPL1
Synonyms	Adapter protein containing PH domain; Adaptor Protein Containing pH Domain; Adaptor protein containing pH domain PTB domain and leucine zipper motif 1; Adaptor protein containing pH domain PTB domain and leucine zipper motif; Adaptor protein phosphotyrosine interacting with PH domain and leucine zipper 1; AKT2 INTERACTOR; APPL 1; Appl1; DCC interacting protein 13 alpha; DCC-interacting protein 13-alpha; DIP 13 alpha; DIP13 alpha; Dip13-alpha; DIP13alpha; DP13A_HUMAN; KIAA1428; PTB domain and leucine zipper motif 1; PTB Domain and Leucine Zipper Motif; Signaling adaptor protein DIP13alpha
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	C-6His
Target Protein Sequence	MPGIDKLPIEETLEDSPQTRSLLGVFEEDATAISNYMNQLYQAMHRIYDAQNELSAATHLTSKLLKEYEKQRFPLGGDDEVMSSTLQQFSKVIDELSSCHAVLSTQLADAMMFPITQFKERDLKEILTLKEVFQIASNDHDAAINRYSRLSKKRENDKVKYEVTEDVYTSRKKQHQTMMHYFCALNTLQYKKKIALLEPLLGYMQAQISFFKMGSENLNEQLEEFLANIGTSVQNVRREMDSDIETMQQTIEDLEVASDPLYVPDPDPTKFPVNRNLTRKAGYLNARNKTGLVSSTWDRQFYFTQGGNLMSQARGDVAGGLAMDIDNCSVMAVDCEDRRYCFQITSFDGKKSSILQAESKKDHEEWICTINNISKQIYLSENPEETAARVNQSALEAVTPSPSFQQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA
Expression Range	1-709aa
Protein Length	Full Length
Mol. Weight	80.6 kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages. Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex.
Subcellular Location	Early endosome membrane; Peripheral membrane protein. Nucleus. Cytoplasm. Endosome. Cell projection, ruffle. Cytoplasmic vesicle, phagosome.
Database References	HGNC: 24035 OMIM: 604299 KEGG: hsa:26060 STRING: 9606.ENSP00000288266 UniGene: PMID: 28902365 APPL1 positively mediated leptin signaling. PMID: 27820851 Decreased expression of APPL1 is associated with polycystic ovary syndrome. PMID: 27075719 Results indicate that persistent rab5 overactivation through beta-cleaved carboxy-terminal fragment of APP-APPL1 interactions constitutes a novel APP-dependent pathogenic pathway in Alzheimer's disease PMID: 26194181 Data show that signal transducing adaptor proteins APPL1 and APPL2 are required for TGFbeta-induced nuclear translocation of TGFbeta type I receptor (TbetaRI)-ICD and for cancer cell invasiveness of prostate and breast cancer cell lines. PMID: 26583432 Low expression of APPL1 is associated with metastasis in prostate cancer. PMID: 26473288 APPL1 endosomes represent a distinct population of Rab5-positive sorting endosomes. PMID: 26459602 article provides evidence that GG genotype and G carrier (CG+GG) genotypes of the rs4640525 polymorphism in the APPL1 gene may be suitable susceptibility biomarkers for NAFLD PMID: 26731990 Two loss-of-function mutations in APPL1, identified by means of whole-exome sequencing, found in two large families with a high prevalence of Familial Diabetes Mellitus. PMID: 26073777 A role for APPL1 in TLR3/4-dependent TBK1 and IKKepsilon activation in macrophages. PMID: 25780039 ATM is the central modulator of APPL-mediated effects on radiosensitivity and DNA repair. PMID: 24763056 APPL1 sensitizes insulin signaling by acting at a site downstream of the IR. Study uncovers a mechanism regulating insulin signaling and crosstalk between the insulin and adiponectin pathways. PMID: 24813896 APPL1 is a positive regulator of Dvl2-dependent transcriptional activity of AP-1. PMID: 25622892 It concludes that APPL1(PH) binding to BAR domain and Reptin is mutually exclusive which regulates the nucleocytoplasmic shuttling of Reptin. PMID: 23891720 The activated EGF receptor enters distinct sub-populations of SNX15- and APPL1-labelled peripheral endocytic vesicles. PMID: 23986476 C-APPL1/A-APPL2 allele combination is associated with non-alcoholic fatty liver disease occurrence, with a more severe hepatic steatosis grade and with a reduced adiponectin cytoprotective effect on liver. PMID: 23977033 TRAF6-mediated ubiquitination of APPL1 is a vital step for the hepatic actions of insulin through modulation of membrane trafficking and activity of Akt. PMID: 23909487 Rab5a and APPL1 are overexpressed in breast cancer, and are positively correlated with the HER-2 expression. PMID: 23291133 neurons with APPL1-positive granules were restricted to the CA1 area and subiculum, areas associated with hippocampal vulnerability, suggesting a possible link between the perisomatic accumulation of APPL1 and Alzheimer's disease. PMID: 23246927 APPL1 regulates basal NF-kappaB activity by modulating the stability of NIK, which affects the activation of p65. PMID: 22685329 analysis of APPL1 and APPL2 proteins and their interaction with Rab PMID: 23055524 APPL1 is a novel target in endoplasmic reticulum (ER) stress-induced insulin resistance and PKCalpha is the kinase mediating ER stress-induced phosphorylation of APPL1 at Ser(430). PMID: 22685300 results demonstrate an important new function for APPL1 in regulating cell migration and adhesion turnover through a mechanism that depends on Src and Akt PMID: 22379109 Genetic variation(s) in APPL1/2 may be associated with CAD risk in T2DM in Chinese population. PMID: 22340213 Treating C2C12 myotubes with adiponectin promoted APPL1 interaction with protein phosphatase 2A (PP2A) and protein kinase Czeta (PKCzeta), leading to the activation of PP2A and subsequent dephosphorylation and inactivation of PKCzeta. PMID: 21835890 These findings suggest that APPL1 is required for EGFR signaling by regulation of EGFR stabilities through inhibition of Rab5. PMID: 22037462 Data suggest that although annexin A2 is not an exclusive marker of APPL1/2 endosomes, it has an important function in membrane recruitment of APPL proteins, acting in parallel to Rab5. PMID: 21645192 APPL1 plays a key role in coordinating the vasodilator and vasoconstrictor effects of insulin by modulating Akt-dependent NO production and ERK1/2-mediated ET-1 secretion in the endothelium. PMID: 21926268 APPL1 abundance is significantly higher in type 2 diabetic muscle; Improvements in hyperglycaemia and hypoadiponectinaemia are associated with reduced skeletal muscle APPL1, and increased plasma adiponectin levels and muscle AMPK phosphorylation. PMID: 21562756 Results show that APPL1 is recruited to aggresomes induced by proteasomal stress, and suggest that proteasome inhibitors in clinical use affect the localization, ubiquitination and solubility of APPL1. PMID: 21320486 The adapter protein APPL1 links FSH receptor to inositol 1,4,5-trisphosphate production and is implicated in intracellular Ca(2+) mobilization. PMID: 21285318 Data indicate APPL1 functions as a scaffolding protein to facilitate adiponectin-stimulated p38 MAPK activation in myotubes. PMID: 20978232 [REVIEW] Emerging roles for AppL1. APPL1 has been shown to interact with a variety of membrane receptors. Recent subcellular localizations of APPL1 place it in dynamic and varied venues in the cell: the cell membrane, the nucleus and the early endosomes PMID: 20600589 Studies indicate that APPL1 has been recently identified as an AdipoR1 and AdipoR2 binding protein. PMID: 20875820 significant fluorescence resonance energy transfer between APPL minimal BAR domain FRET pairs PMID: 20814572 The promyogenic function of Cdo involves a coordinated activation of p38MAPK and Akt via association with scaffold proteins, JLP and Bnip-2 for p38MAPK and APPL1 for Akt. PMID: 20484574 Rab5a promoted proliferation of ovarian cancer cells, which may be associated with the APPL1-related epidermal growth factor signaling pathway. PMID: 20412119 Used mass spectrometry (MS) to identify 13 phosphorylated residues within APPL1. PMID: 20095645 identification of a pathway directly linking the small GTPase Rab5, a key regulator of endocytosis, to signal transduction and mitogenesis via APPL1 and APPL2, two Rab5 effectors PMID: 15016378 APPL1 is a potential interactor with FSHR PMID: 15070827 APPL1 interacts with adiponectin receptors in mammalian cells and the interaction is stimulated by adiponectin. PMID: 16622416 APPL1 acts as a common downstream effector of Adiponectin receptors R1 and -R2, mediating adiponectin-evoked endothelial nitric oxide production and endothelium-dependent vasodilation. PMID: 17287464 The ability of APPL1 to interact with multiple signaling molecules and phospholipids supports an important role for this adaptor in cell signaling. PMID: 17502098 The crystal structures of human APPL1 N-terminal BAR-PH domain motif, is reported. PMID: 17581628 These data suggest that APPL1 plays an important role in insulin-stimulated Glut4 translocation in muscle and adipose tissues and that its N-terminal portion may be critical for APPL1 function. PMID: 17848569 The findings suggest a role for APPL1 and APPL2 protein as dynamic scaffolds that modulate RAB5-associated signaling endosomal membranes by their ability to undergo domain-mediated oligomerization, membrane targeting and phosphoinositide binding. PMID: 18034774 Thus, binding to APPL1 helps localize OCRL at specific cellular sites, and disruption of this interaction may play a role in disease. PMID: 18307981 Adiponectin blocks interleukin-18-mediated endothelial cell death via APPL1-dependent AMP-activated protein kinase (AMPK) activation and IKK/NF-kappaB/PTEN suppression. PMID: 18632660 APPL proteins exert their stimulatory effects on beta-catenin/TCF-dependent transcription by decreasing the activity of a Reptin-containing repressive complex PMID: 19433865 Adiponectin activates AMP-activated protein kinase in muscle cells via APPL1/LKB1-dependent and phospholipase C/Ca2+/Ca2+/calmodulin-dependent protein kinase kinase-dependent pathways PMID: 19520843

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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