Recombinant Human Cytomegalovirus Immediate Early Protein Ie1 (UL123) Protein (His&Myc)

Name: Recombinant Human Cytomegalovirus Immediate Early Protein Ie1 (UL123) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-00465P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Cytomegalovirus Immediate Early Protein Ie1 (UL123) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	F5HCM1
Target Symbol	UL123
Species	Human cytomegalovirus (strain Merlin) (HHV-5) (Human herpesvirus 5)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFEQVTEDCNENPEKDVLTELVKQIKVRVDMVRHRIKEHMLKKYTQTEEKFTGAFNMMGGCLQNALDILDKVHEPFEDMKCIGLTMQSMYENYIVPEDKREMWMACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYRNIEFFTKNSAFPKTTNGCSQAMAALQNLPQCSPDEIMSYAQKIFKILDEERDKVLTHIDHIFMDILTTCVETMCNEYKVTSDACMMTMYGGISLLSEFCRVLCCYVLEETSVMLAKRPLITKPEVISVMKRRIEEICMKVFAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVAYTLATAGASSSDSLVSPPESPVPATIPLSSVIVAENSDQEESEQSDEEQEEGAQEEREDTVSVKSEPVSEIEEVASEEEEDGAEEPTASGGKSTHPMVTRSKADQ
Expression Range	1-491aa
Protein Length	Full Length
Mol. Weight	62.6 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays an important role in transactivating viral early genes as well as activating its own promoter, probably by altering the viral chromatin structure. Expression of IE1 and IE2 proteins is critical for the establishment of lytic infection and reactivation from viral latency. Disrupts PML-associated ND10 nuclear bodies by interfering with host PML and SP100 sumoylation thereby altering the regulation of type I and type II interferon-induced gene expression. Promotes efficient viral growth by interacting with and directing host SP100 to degradation, leading to enhanced acetylation level of histones. In addition, functions in counteracting the host innate antiviral response. Inhibits the type I interferon pathway by directly interacting with and sequestrating host STAT2. Also targets type II interferon pathway by repressing IL6- and STAT3 target genes. Repression of STAT3 genes is due to STAT3 nuclear accumulation and disruption of IL6-induced STAT3 phosphorylation by IE1. This repression is followed by phosphorylation and activation of STAT1. Inhibits host ISG transcription by sequestering host ISGF3 in a PML- and STAT2- binding dependent manner. Alters host cell cycle progression, probably through its interaction with host E2F1 or RB1 that overcomes the RB1-mediated repression of E2F-responsive promoters.
Subcellular Location	Host nucleus.
Protein Families	HHV-5 IE1 protein family
Database References	KEGG: vg:3077513

Gene Functions References

Human cytomegalovirus IE1 causes SOX2 downregulation by promoting the nuclear accumulation and inhibiting the phosphorylation of STAT3, a transcriptional activator of SOX2 expression. PMID: 29950413
IE1 is involved in Hes1 degradation by assembling a ubiquitination complex and promoting Hes1 ubiquitination as a potential E3 ubiquitin ligase, followed by proteasomal degradation of Hes1. PMID: 28750047
IE2 disrupts the orderly process of brain development in a stepwise manner, furthering our understanding of neurodevelopmental human cytomegalovirus pathogenesis PMID: 28615204
data reveal that MICA and PVR are directly regulated by human cytomegalovirus immediate early proteins, and this may be crucial for the onset of an early host antiviral response PMID: 27733551
findings demonstrate that the PML protein, which mediates an intrinsic immune response against human cytomegalovirus, specifically serves as an E3 ligase for SUMO modification of IE1p72 PMID: 28250117
we present transcriptome data indicating that IE1 is as significant a repressor as it is an activator of host gene expression PMID: 27387064
These data suggest that targeting PML by human herpesvirus 5 IE1 represents a strategy to antagonize both intrinsic and innate immune mechanisms. PMID: 26559840
co-immunoprecipitation experiments demonstrate that IE1CORE binds via the coiled-coil domain to PML and also interacts with TRIM5alpha PMID: 25412268
IE1- and pp71 mediated viral strategies against cellular defenses.[review] PMID: 25501994
Human cytomegalovirus IE1 exon 4 interacts with Topoisomerase IIbeta (TOPOIIbeta), whose activity is required for viral genome persistence and maintenance via binding to a cis-acting viral maintenance element. PMID: 25011107
IE1 and IE2 are required for efficient human cytomegalovirus gene expression. PMID: 25552717
Together, these results indicate that cytomegalovirus IE1 disrupts IFNgamma signaling by interfering with signaling events in the nucleus through a novel mechanism. PMID: 24699362
GST pulldown experiments revealed that both IE72 and wt p53 bound the important homology-directed repair protein, Rad51. PMID: 24576846
Antisense transcripts of UL123 were packaged in highly purified virions. PMID: 23884634
IE1 protein targets host chromosomes by docking to the acidic pocket on the nucleosome surface. PMID: 24227840
pp71 stimulates major histocompatibility complex class i presentation of IE1-derived peptides at immediate early times of infection. PMID: 23449799
These results suggest that the effects of cytomegalovirus IE1 and pp71 on subsets of ND10 components combine to mirror the overall activities of herpes simplex virus 1 ICP0. PMID: 23135716
Human cytomegalovirus IE1/2 expression was downregulated by cyclin A2, CDK1 and CDK2. PMID: 22718829
chromatin-tethering domain required for associations of IE1, PML and STAT2 with mitotic chromosomes, but not essential for viral replication PMID: 22158879
These findings indicate that thehuman herpesvirus 5 IE1-dependent loss of human Sp100 proteins during virus infection may represent an important requirement for efficient viral growth. PMID: 21880768

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.