Recombinant Human Cytokine-Inducible Sh2-Containing Protein (CISH) Protein (GST)

Name: Recombinant Human Cytokine-Inducible Sh2-Containing Protein (CISH) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-03702P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Cytokine-Inducible Sh2-Containing Protein (CISH) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9NSE2
Target Symbol	CISH
Synonyms	BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL
Expression Range	1-258aa
Protein Length	Full Length
Mol. Weight	55.7kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Database References	HGNC: 1984 OMIM: 602441 KEGG: hsa:1154 STRING: 9606.ENSP00000409346 UniGene: Hs.655334
Tissue Specificity	Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.

Gene Functions References

identified P2RX2, KCNQ5, ERBB3 and SOCS3 to be associated with the progression of age-related hearing impairment PMID: 29325454
CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK. PMID: 28038963
Studies indicate that suppressors of cytokine signaling (SOCS) proteins CIS, SOCS1, and SOCS3 can be considered the third immunocheckpoint molecules since they regulate cytokine signals that control the polarization of CD4(+) T cells and the maturation of CD8(+) T cells. PMID: 28188673
CISH rs414171 and rs6768300 variants might be associated with protection from pulmonary tuberculosis in Zahedan, Southeast Iran. PMID: 27266592
This study showed that there was significantly increased levels of CIS mRNA in elderly and Alzheimer's disease brains. PMID: 25286386
Data indicate that CIS protein stability is regulated through multiple mechanisms, including ubiquitination and interaction with Elongin B/C proteins, whereas CIS functional inhibition of PRLR signaling is dependent on the Elongin B/C interaction. PMID: 25448846
SOCS single nucleotide polymorphism is associated with breast cancer. PMID: 25104439
Study in Chinese Han population confirms that previously identified variants of CISH are associated with susceptibility to pulmonary tuberculosis. PMID: 25460819
CISH gene polymorphisms at -292 (rs414171) are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase, and AA is a favorable genotype for this effect. PMID: 24791876
Two SNPs (rs414171 and rs2239751) in the CISH gene were associated with persistent HBV infection in Han Chinese population PMID: 24964072
two genetic variants in CISH gene appear to increase susceptibility to TB in Chinese Han population PMID: 23949851
CISH promoter rs414171 and rs809451 polymorphisms may play a vital role in mediating individual susceptibility to tuberculosis PMID: 24632804
methylation of SOCS1, SOCS2, SOCS3 and CISH is infrequent in Ph-ve MPN. PMID: 24131863
data suggest FXR-mediated upregulation of cytokine inducible SH2-containing protein (CISH) may play an important role in the homeostasis of cytokine signal networks and be beneficial to control cytokine-associated inflammatory diseases PMID: 22817871
In GH transduction defect, abnormal GH signalling may be caused by over-expression of CIS, which may increase degradation of GHR PMID: 23426819
Suppressors of both cytokine signaling SOCS7 and cytokine-inducible SH2 protein (CIS) are expressed constitutively at higher levels in LNCaP-S17 (prostate cancer) cells than in LNCaP-C3 cells. PMID: 22213096
Study indicates the vital role of CISH single nucleotide polymorphism (SNP)-292A>T variant to hepatitis B virus infection in a Vietnamese population. PMID: 22033525
The authors conclude that Mycobacterium tuberculosis infection induces development of Tregs from CCR4(+) cells through a process that depends on PD-1and CISH. PMID: 21383382
Variants of CISH are associated with susceptibility to diseases caused by diverse infectious pathogens. PMID: 20484391
Low level of CISH expression in various tissues suggests that it is a novel prospective marker for diagnosis of colon cancer. PMID: 19807022
Up-regulation of cytokine inducible SH2-containing protein is associated with adult T-cell leukemia PMID: 14630083
CIS (cytokine-inducible SH2 protein)is binding with the conserved Y985 and Y1077 motifs in the cytosolic domain of the leptin receptor. PMID: 16684815
analysis of STAT5, CIS, and SOCS2 interactions with the growth hormone receptor PMID: 17666591
RACK1 has a role in protecting cancer cells from apoptosis by regulating the degradation of BimEL, which together with CIS could play an important role of drug resistance in chemotherapy PMID: 18420585
Elongin B/C recruitment regulates substrate binding by CIS PMID: 18508766
CISH expression in allergen-stimulated CD4(+) T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children. PMID: 18647318
CIS can serve as an SLE disease marker and may be involved in the pathogenesis of SLE, and that TNF-alpha may play an important role in the regulation of CIS PMID: 18820827
miR-98 and let-7 confer cholangiocyte expression of CIS in response to microbial challenge, a process that may be relevant to the regulation of TLR-mediated epithelial innate immune response. PMID: 19592657
These results suggest that RSV infection escapes the innate antiviral response by inducing SOCS1, SOCS3 or CIS expression in epithelial cells. PMID: 19595407

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.