Recombinant Human CyclinA2 Protein

Name: Recombinant Human CyclinA2 Protein
Brand: Beta LifeScience
SKU: BL-1551SG
Availability: InStock

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	NM_001237
Synonym	CCNA2; CCN1; CCNA
Background	CyclinA2 belongs to the highly conserved cyclin family whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle and function as regulators of CDKs. CyclinA2 is an essential component of all embryonic and somatic cell cycles in mammals (1). CyclinA2 is highly expressed in the testis and certain myeloid leukemia cells (2). Cyclin A also plays a major role in the control of DNA replication (3). CyclinA2 binds and activates CDC2 or CDK2 and thus promotes both cell cycle G1/S and G2/M transitions.
Description	Recombinant full-length human CyclinA2 was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source	Sf9 insect cells
AA Sequence	Full Length
Molecular Weight	~78 kDa
Purity	For specific purity information on a given lot, see related COA.
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Formulation	Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability	The recombinant protein is stable for up to 12 months at -70°C
Usage	For Research Use Only
Storage	Recombinant Human CyclinA2 Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
Subcellular Location	Nucleus. Cytoplasm.
Protein Families	Cyclin family, Cyclin AB subfamily
Database References	HGNC: 1578 OMIM: 123835 KEGG: hsa:890 STRING: 9606.ENSP00000274026 UniGene: PMID: 30106440 Study demonstrates that cyclin A2 interacts with actin and RhoA during mitosis, and that cyclin A2 depletion induces a dramatic decrease in active RhoA in mitosis suggesting that cyclin A2 participation in RhoA activation in late mitosis. PMID: 27279564 Bisphosphonate inhibits the expression of cyclin A2 at the transcriptional level in normal human oral keratinocytes. PMID: 28713904 the presented evidence suggested that WA can inhibit HCC cell proliferationand tumorigenesis through miR-22-repressed CCNA2, which was at least partially through FXR regulation PMID: 27738335 Findings suggest that ERK1/2-mediated Cdk2/cyclin A signaling pathway is involved in 7-hydroxy-5,4'-dimethoxy-2-arylbenzofuran (Ary) - induced G1/S-phase arrest. PMID: 27259234 some cancer cells are defective for efficient interaction between p27 and CycA-CDK complex due to qualitative alteration(s) PMID: 29098944 This study shows that cocaine induces significant increases in both the astrocytic expression of cyclin A2 and the proliferation of primary human astrocytes. PMID: 27834787 Low CCNA expression is associated with lung cancer progression. PMID: 27694898 inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426 An increase of cyclin A2 expression was identified for the first time in a case of splenic diffuse red pulp small B-cell lymphoma. PMID: 27761608 Consistent with these findings, a genome-scale pooled RNA interference screen revealed that toxic doses of MK-1775 are suppressed by CDK2 or Cyclin A2 knockdown. These findings support G2 exit as the more significant effect of Wee1 inhibition in pancreatic cancers. PMID: 26890070 Clioquinol suppressed cell cycle progression in the S-phase in SMMC-7721 hepatoma cells via the p21, p27-cyclin E,A/Cdk2 pathway. PMID: 26677902 On analyzing cyclin A and B1 (CCNA and CCNB1) expression, positive staining in 90% cases of PTC were observed. The study revealed a significant difference in expression of cyclins A and B1 between classic and non-classic variants of PTC. PMID: 26706989 Study describes a positive feedback loop centred on cyclin A2-Cdk2 inhibition of interphase APC/C-Cdc20 to allow further cyclin A2 accumulation and mitotic entry. PMID: 26960431 PIWIL2 has a role in promoting progression of non-small cell lung cancer by inducing CDK2 and Cyclin A expression PMID: 26373553 Cyclin A2-binding function of pUL21a contributes to the maintenance of a cell cycle state conducive for the completion of the human cytomegalovirus (HCMV) replication cycle. PMID: 25393019 HDAC10 regulates cyclin A2 expression by deacetylating histones near the let-7 promoter. PMID: 26240284 Results suggest that cyclin A2 overexpression may drive cancer by promoting AKT-dependent proliferation and survival. PMID: 24795023 for the first time, we demonstrated that CTD induced G2/M phase arrest via the inhibition of Cdc25c and cyclin A and induced apoptosis was through death receptor (extrinsic), intrinsic (mitochondria) and ER stress pathways in A375. S2 cells. PMID: 25340978 CCN2 is a regulator of the transition through cytokinesis during terminal erythropoiesis. PMID: 25615569 CCNA2 is a biomarker for the prognosis of ER+ breast cancer and monitoring of tamoxifen efficacy PMID: 24622579 our data showed that bile acid-activated FXR stimulates miR-22-silenced CCNA2, a novel pathway for FXR to exert its protective effect in the gastrointestinal tract. PMID: 25596928 Expression of cycle marker cyclin A1 differs between benign and malignant papillary breast lesions. PMID: 25501285 Cyclin A2 and its associated kinase (cdk2) activity are required for optimal induction of progesterone receptor target genes in breast cancer cells. PMID: 25220500 It is concluded that non-response to everolimus is characterized by increased cdk2/cyclin A, driving RCC cells into the G2/M-phase. VPA hinders everolimus non-response by diminishing cdk2/cyclin A PMID: 24935000 cyclin A expression predicted 100% of the patients at risk for metastasis of rectal neuroendocrine tumors. PMID: 24824027 The aim of the present study was to evaluate the prognostic value of the proliferation factors mitotic activity index (MAI), phosphohistone H3 (PPH3), cyclin B1, cyclin A and Ki67, alone and in combinations. PMID: 24324728 show that cyclin A is an independent prognostic factor in endometrioid adenocarcinoma PMID: 24519066 level unchanged in endometrial cancer PMID: 23815208 APPOLON to be a novel regulator of mitotic CYCLIN A degradation independent of SAC. PMID: 24302728 Data indicate that the major tegument 150-kDa phosphoprotein (pp150) of human cytomegalovirus (HCMV) binds to cyclin A2 via a functional RXL/Cy motif resulting in its cyclin A2-dependent phosphorylation. PMID: 24101496 HDAC3 regulates cyclin A stability. PMID: 23760262 Cyclin A creates a cellular environment that promotes microtubule detachment from kinetochores in prometaphase to ensure efficient error correction and faithful chromosome segregation PMID: 24013174 We further detected that reduced level of GRK2 induced a small cell cycle arrest at G2/M phase by enhancing the expression of cyclin A PMID: 23460259 Overexpression of cyclin A2 is associated with stage I non-small cell lung cancer. PMID: 23115005 human papillomavirus E4 proteins can interact with cyclin A and cdk2, which may contribute to viral manipulation of the host cell cycle. PMID: 23065011 The expression of cell proliferation markers cyclin A and p27 are independent prognostic factors in patients with esophageal squamous cell carcinoma. PMID: 21762277 The study has revealed that some highly differentiated human tissues express an intron-retaining cyclin A2 mRNA that induced a G1/S block in vitro. PMID: 22745723 Human cytomegalovirus IE1/2 expression was downregulated by cyclin A2, CDK1 and CDK2. PMID: 22718829 cyclin A and cyclin B1 are useful markers in the distinction of benign and malignant thyroid tumors PMID: 21264543 GC box was responsible for the cyclic activity of human CKAP2 promoter through the phosphorylation of Sp1, possibly by Cyclin A/Cdk complex. PMID: 22465120 Data demonstrated that in addition to galectin-3, HK III and cyclin A profiles could be important biomarkers in predicting malignancy in follicular thyroid nodules. PMID: 17868400 Cyclin A2 negatively controls cell motility by promoting RhoA activation, thus demonstrating a novel Cyclin A2 function in cytoskeletal rearrangements and cell migration. PMID: 22232705 NF-Y binds to CCAAT sequences in the Cyclin A promoter, as well as to those in the promoters of cell cycle G2 regulators such as CDC2, Cyclin B and CDC25C. PMID: 21871181 These data demonstrate that B55alpha acts to antagonize Cyclin A/Cdk-dependent activation of FoxM1, to ensure that FoxM1 activity is restricted to the G(2) phase of the cell cycle. PMID: 21813648 The current results suggested that an SNP (rs769236) at the promoter of CCNA2 may be associated significantly with increased risk of colon, liver, and lung cancers. PMID: 21858804 The expression of mRNA and protein of Cyclin A in hypertrophic scar changes from high level to low level as the hypertrophic scar develops, while the expression of P21cip1 changes from low level to high level. PMID: 21046779 Low p16(Ink4a) expression, overexpression of p53, cytoplasmic p27(Kip1), and Cyclin A are predictive markers for shorter overall survival in ovarian carcinomas. PMID: 21464733 cyclin A was strongly associated with the expression of cyclin E in invasive ductal breast carcinoma PMID: 21472690 Cdc20 requires APC3 and APC8 to bind and activate the APC/C when the spindle assembly checkpoint is satisfied, but only APC8 when active, and APC10 is crucial for the destruction of cyclin B1 and securin, but not cyclin A PMID: 21336306

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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