Recombinant Human CPE Protein (C-6His)

Name: Recombinant Human CPE Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-1441NP
Availability: InStock

BL-1441NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Carboxypeptidase E is produced by our Mammalian expression system and the target gene encoding Arg42-Ser453 is expressed with a 6His tag at the C-terminus.
Accession	P16870
Synonym	Carboxypeptidase E(CPE for short); Carboxypeptidase H; Enkephalin convertase; Prohormone-processing carboxypeptidase
Gene Background	The active form of CPE cleaves C-terminal amino acid residues of the peptide, and is thus involved in the biosynthesis of peptide hormones and neurotransmitters including insulin, enkephalin, etc. It is thought that membrane-associated CPE acts as a sorting receptor for targeting regulated secretory proteins which are mostly prohormones and neuropeptides in the trans-Golgi network of the pituitary and in secretory granules into the secretory pathway. Defects in this protein are implicated in type II diabetes due to impaired glucose clearance and insulin resistance.
Molecular Mass	47.2 KDa
Apmol Mass	50-60 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM ZnCl2, 10% Glycerol, pH 7.5.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Sorting receptor that directs prohormones to the regulated secretory pathway. Acts also as a prohormone processing enzyme in neuro/endocrine cells, removing dibasic residues from the C-terminal end of peptide hormone precursors after initial endoprotease cleavage.
Subcellular Location	[Isoform 1]: Cytoplasmic vesicle, secretory vesicle. Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted.
Protein Families	Peptidase M14 family
Database References	HGNC: 2303 OMIM: 114855 KEGG: hsa:1363 STRING: 9606.ENSP00000386104 UniGene: PMID: 28656234 This study has uncovered a human CPE/NF-alpha1 gene mutation that could lead to comorbidity of dementia and depression, emphasizing the importance of this gene in cognitive function. PMID: 27922637 CPE through its N'-terminal sequence, forms aggregates with Wnt3a and possible endoplasmic reticulum (ER) stress leading to its loss of function. PMID: 27375026 we have identified a novel SNP in the CPE gene which results in the loss of its neuroprotective function in cells and may confer neurological disorders in humans PMID: 28114332 High-level CPE [ carboxypeptidase E] expression was associated with a poor prognosis in early-stage cervical cancer. CPE may serve as a biomarker for predicting PLNM [ pelvic lymph node metastasis ] and survival in these patients. PMID: 26695643 Low carboxypeptidase E expression is associated with recurrence in early-stage hepatocellular carcinoma. PMID: 26803519 Down-expression of liver carboxypeptidase E may reduce the secretion of serum cholecystokinin and contribute to the formation of cholesterol gallstone. PMID: 26228366 Downregulation of CPE regulates cell proliferation and chemosensitivity in pancreatic cancer. PMID: 25374060 Disruption of insulin receptor (IR) expression in beta cells has a direct impact on the expression of the convertase enzyme carboxypeptidase E (CPE) by inhibition of the eukaryotic translation initiation factor Eif4g1. PMID: 24843127 Upregulation of CPE promotes cell proliferation and tumorigenicity in colorectal cancer. PMID: 24006921 Data suggest that splice variant of carboxypeptidase E (CPE-DeltaN) that CPE-DeltaN expression might be a potential prognostic marker for colorectal cancer patients. PMID: 23852859 CPE is essential in the process and targeting of neuropeptides and neurotrophins, its participation in the pathological progression of Alzheimer's disease may be suggested PMID: 22998035 CPE forms a complex, probably through sequences located at its N-terminal domain, with Wnt3a and the extracellular cysteine rich domain of Fz1. PMID: 22824791 Neither high glucose nor insulin (with low glucose) regulates beta-cell CPE (but either up-regulates CPD). PMID: 21628999 an N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers PMID: 21285511 CPE may play a role in promoting tumor growth and invasion. PMID: 21061162 Carboxypeptidase E is differently expressed in subcutaneous and visceral fat of obese subjects PMID: 12530526 protein binding with Con A in seminal plasma PMID: 14690244 cDNA microarray analysis led to the identification of 2 novel biomarkers that should facilitate molecular diagnosis and further study of pulmonary neuroendocrine tumors. PMID: 15492986 A possible role for mutations in CPE in the development of coronary heart disease. PMID: 17957445 the severity of the coronary atherosclerosis estimated by Gensini score was significantly influenced by the presence of the A2925G mutant and G2855A mutant of the CPE gene PMID: 18080843 polymorphism in the CPE exon5 gene may contribute to the angiographical characteristics of coronary atherosclerosis in the Chinese population PMID: 18501121 Carboxypeptidase E degradation contributes to palmitate-induced beta-cell ER stress and apoptosis; CPE is a major link between hyperlipidemia and beta-cell death pathways in diabetes. PMID: 18550819 CPH-Abs may allow discrimination of a more latent subset of adult-onset autoimmune diabetes (LADA). PMID: 19120309 Carboxypeptidase E may be a key molecule to regulate Caspr2 (carboxypeptidase E) trafficking to the cell membrane. PMID: 19166515 missense polymorphism encoding altered activity PMID: 11462236

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.