Recombinant Human Cell Adhesion Molecule 1 (CADM1) Protein (His), Active

Name: Recombinant Human Cell Adhesion Molecule 1 (CADM1) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05702P
Availability: InStock

Greater than 95% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2μg/mL can bind Human CRTAM ，the EC 50 is 17.82-21.71 ng/mL. Biological Activity Assay

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Product Overview

Description	Recombinant Human Cell Adhesion Molecule 1 (CADM1) Protein (His), Active is produced by our Mammalian cell expression system. This is a protein fragment.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized Human CADM1 at 2 μg/mL can bind Human CRTAM , the EC50 is 17.82-21.71 ng/mL.
Uniprotkb	Q9BY67
Target Symbol	CADM1
Synonyms	Cell adhesion molecule 1 ; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Spermatogenic immunoglobulin superfamily; SgIgSF; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	C-10His
Target Protein Sequence	QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDH
Expression Range	45-374aa
Protein Length	Partial
Mol. Weight	38.5kDa
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node. Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells. Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. Acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. May contribute to the less invasive phenotypes of lepidic growth tumor cells.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cell junction, synapse.
Protein Families	Nectin family
Database References	HGNC: 5951 OMIM: 605686 KEGG: hsa:23705 STRING: 9606.ENSP00000395359 UniGene: PMID: 29734164 High CADM1 expression is associated with KSHV infection. PMID: 29698475 the immunohistochemical expression of CADM1 has the potential to contribute as an auxiliary tool to the differential diagnosis between nevi and melanomas. PMID: 28843749 These findings suggest that CADM1 expression is a novel prognostic factor for peripheral T cell lymphoma, not otherwise specified PMID: 28940014 miR-21 enhances chemo-resistance in tongue cancer cells via directly targeting CADM1, and an inverse correlation between miR-21 and CADM1 expression in vivo. MiR-21 overexpression is attributed to MYCN-mediated transcriptional regulation, which is also predictive for a worse prognosis in tongue cancer PMID: 27055844 We conclude that CADM1 was not associated with cell-cell interaction between lymphoma cells and macrophages, although CADM1 may be a useful marker of ATLL for diagnostic procedures. PMID: 28420814 miR-21 overexpression promotes cardiac fibrosis via STAT3 signaling pathway by decrease CADM1 expression, indicating miR-21 as an important signaling molecule for cardiac fibrotic remodeling and atrial fibrillation. PMID: 28335740 Pathway analysis of the genes associated with the 46 CpG sites revealed an enrichment of immune system process genes, including LYST (cg16962115, FDR = 1.24E-04), CADM1 (cg21933078, FDR = 1.22E-02) and NFATC1 (cg06784563, FDR = 1.46E-02) PMID: 28637314 CADM1 mRNA expression was highly upregulated in normal tissues compared to esophageal squamous cell carcinoma tissues, indicating that the loss of CADM1 expression influenced the pathogenesis, invasion, and metastasis of ESCC. PMID: 28510248 Eight and five of the nine samples were negative for cell adhesion molecule 1 and Osterix respectively. The other markers showed no statistical significance(CD151,ALP). osteoblastic differentiation can occur in carcinoma cells and that cell adhesion molecule 1 could be a useful marker for identifying this phenomenon in carcinoma tissues PMID: 28651491 Our results suggest that, as in the CNS, CADM1 interactions drive exocytic site assembly and promote actin network formation. These results support the broader hypothesis that the effects of cell-cell contact on beta-cell maturation and function are mediated by the same extracellular protein interactions that drive the formation of the presynaptic exocytic machinery. These interactions may be therapeutic targets for re... PMID: 27072493 High expression of CADM1 on most HTLV-1-infected cells in the face of enhanced cytotoxic T lymphocyte counterselection implies that CADM1 confers a strong benefit on the virus. PMID: 27105228 Decreased expression of TSLC1 is associated with the differentiation in ovarian epithelial cancer. TSLC1 might be used as a molecular marker of severity in early stage ovarian cancer, and to help differentiating benign and malignant ovarian tumors. PMID: 27146728 Suppression of tumorigenesis by CADM1 may be mediated by the Rb-E2F1 pathway. PMID: 27298105 CADM1 prevents squamous cell carcinoma progression by reducing STAT3 activity PMID: 27035095 Decreased expression of TSLC1 is correlated with the malignancy of non-muscle invasive bladder cancer tissues and low TSLC1 expression may serve as a predictor for bladder cancer recurrence and progression. PMID: 26894268 CADM1 deletion is associated with neuroblastoma and ganglioneuroma. PMID: 26463893 Hypermethylation of CADM1 gene may be highly associated with the development of cervical cancer. PMID: 26552048 Our study showed that miR-126, by down-regulation CADM1, enhances migration and invasion in gastric cancer cells. PMID: 26464628 This study revealed that Merkel cell polyomavirus -negative Merkel cell carcinomas significantly expressed higher CADM1 and lower MAL than Merkel cell polyomavirus -positive Merkel cell carcinomas PMID: 26772392 CADM1/MAL methylation increases with severity of cervical intraepithelial neoplasia PMID: 26219541 SynCAM1 delineates the postsynaptic perimeter of the synaptic cleft as determined by immunoelectron microscopy. PMID: 26687224 increased CADM1 shedding appeared to contribute to the development of emphysema by generating alphaCTF and the ICD in lung epithelial cells. PMID: 26259600 CADM1 shedding is increased in idiopathic interstitial pneumonia and contributes to alveolar epithelial cell apoptosis. PMID: 26231557 Results show that CADM1 is downregulated in HPV-positive cervical cancers and that epigenetic alteration of CADM1 was more frequent in this type of cancer. The restoration of CADM1 expression may be a potential strategy for cervical cancer therapy. PMID: 25845528 The use of CADM1, MAL, and MIR124-2 as biomarkers for full molecular screening in HIV infected women who are also positive for human papillomavirus. PMID: 26473640 CADM1 induction was repressed in primary samples from AML patients at relapse. PMID: 25491945 Our study reveals a novel mechanism of chronic NF-kappaB activation by CADM1 in HTLV-1 infected cells PMID: 25774694 Trans-homophilic interaction of CADM1 activates PI3K by forming a complex with MAGuK-family proteins MPP3 and Dlg. PMID: 25268382 In the absence of SynCAMs, selective axon-axon interactions are perturbed resulting in aberrant pathfinding of sensory axons. PMID: 25335893 Increased ectodomain shedding of cell adhesion molecule 1 from pancreatic islets in type 2 diabetic pancreata. PMID: 24964098 In a population-based association study, we confirm a role for CADM1 variants in increasing the risk of VT by interaction with protein C pathway abnormalities. PMID: 25306186 The significant association of IGSF4 with HPV-positive head and neck squamous cell carcinoma suggests that HPV-positive tumors are to a greater extent driven by promoter hypermethylation in this tumor suppressor gene. PMID: 25569158 HPV-positive oropharyngeal squamous cell carcinoma is associated with TIMP3 and CADM1 promoter hypermethylation. PMID: 25065733 CADM1/TSLC1 is an antiinvasive gene, the overexpression of which inhibits the invasion of A375 cells. PMID: 25215547 Cytology and bi-marker CADM1/MAL-methylation analysis perform complementary for CIN2+/CIN3+ detection when used as triage tool on cervical scrapes of HPV positive women. PMID: 25667975 TSLC1-AS1 was a tumor suppressor of glioma and a mediator of TSLC1 expression. PMID: 25031725 The study showed that CADM1 is a direct target of miR-1246 leading to its down regulation in hepatocellular carcinoma cells. PMID: 25159494 DNA methylation analysis of CADM1, MAL and miR124-2 in cervical scrapes consistently detects cervical cancer PMID: 25281766 Loss of CADM1 expression is an independent prognostic factor especially associated with the development of brain metastases in breast cancer patients. PMID: 24833255 We showed that the CADM1 versus CD7 plot is capable of discriminating clonally expanding HTLV-I-infected cells in indolent and acute-type T-cell leukemia-lymphomas and in asymptomatic carriers PMID: 24727323 MPP3 and Dlg, membrane-associated guanylate kinase homologs (MAGuK) proteins, connect CADM1 with p85 of PI3K by forming a multi-protein complex at the periphery of cells. PMID: 24503895 Data suggest that cell adhesion molecule 1 (CADM1) is a key adhesion receptor, which regulates mast cells (MCs) net adhesion. PMID: 24465823 TGF-beta upregulates miR-182 expression to promote gallbladder cancer metastasis by targeting CADM1. PMID: 24445397 CADM1 ectodomain shedding appeared to cause alveolar cell apoptosis in emphysematous lungs by producing alphaCTF that accumulated in mitochondria. PMID: 24092566 The paper shows that CADM1 regulates mast cell net adhesion, both directly through CADM1-dependent adhesion, and indirectly through the regulation of other adhesion receptors. The latter is likely to occur via docking of KIT and polymerization of cortical F-actin. CADM1 is involved in docking of KIT to the cell surface and phosphotyrisine signaling, and polymerization of cortical F-actin. PMID: 24465823 SynCAM, a novel putative tumor suppressor, suppresses growth and invasiveness of glioblastoma. PMID: 23907434 Infection of NCI-H460, A549, and H1299 lung cancer cells with Ad.sp-E1A(Delta24)-TSLC1 induced apoptosis. PMID: 23503473 Data suggest that tumor suppressor in lung cancer 1 (TSLC1) as tumor suppressor may play prominent roles in development and progression of cutaneous squamous cell carcinoma (CSCC) via regulation of different biological processes. PMID: 23812766 The cell adhesion molecule Necl-2 competitively binds the immune receptor CRTAM. PMID: 23871486

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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