Recombinant Human CDC25B Protein

Beta LifeScience SKU/CAT #: BL-0221SG
Recombinant Human CDC25B Protein

Recombinant Human CDC25B Protein

Beta LifeScience SKU/CAT #: BL-0221SG
Catalog No.: BL-0221SG

Product Overview

Tag GST
Host Species Human
Accession NM_021873
Synonym none
Background CDC25B (also known as cell division cycle 25 homolog B) is a member of the CDC25 family of phosphatases that activate the cyclin dependent kinase CDC2 by removing two phosphate groups. CDC25B is required for cell entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. Cdc25B is the first genetic model for studying the mechanisms regulating prophase arrest in vertebrates (1). The regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation (2).
Description Full-length recombinant human CDC25B was produced in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence Full Length
Molecular Weight ~110 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human CDC25B Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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