Human CBFB (Core-Binding Factor Subunit Beta) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-00421P
SDS-PAGE analysis of Human CBFB (Core-Binding Factor Subunit Beta) - Recombinant Protein, CAT# BLT-00421P, showing >95% purity under 15% SDS-PAGE (Reduced)
Human CBFB (Core-Binding Factor Subunit Beta) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-00421P
Regular price
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$595.00
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Quantity Pricing
| Pack Size | Price (USD) |
|---|---|
| 500 µg | $1,375 |
| 1 mg | $2,245 |
For direct online orders, quantity pricing will be displayed in cart when you add 5x100ug or 10x100ug
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Product Overview
| Product Name | Recombinant Human Core Binding Factor Beta Subunit (CBFb) Protein |
| Product Overview | This recombinant human Core Binding Factor Beta Subunit (CBFb) protein includes amino acids 1-133aa of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5% trehaloseprior to lyophilization. |
| Target Uniprot Id | Q13951 |
| Recommended Name | Core-binding factor subunit beta |
| Gene Name | CBFB |
| Synonyms | Core-binding factor, beta subunit |
| Species | Human |
| Predicted Molecular Mass | 25 kDa |
| Expression System | E.coli |
| Expression Range | 1-133aa |
| Tag | N-6His |
| Purity | >95% |
| Formulation | Lyophilized |
| Buffer | Phosphate buffered saline (pH7.4) containing 0.01% sarcosyl, 5%Trehalose |
| Storage Condition | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications | Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area | Epigenetics And Nuclear Signaling |
| Target Function | Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. |
| Subcellular Location | Nucleus. |
| Protein Family | CBF-beta family |
| Associated Diseases | A chromosomal aberration involving CBFB is associated with acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein that consists of the 165 N-terminal residues of CBF-beta (PEPB2) with the tail region of MYH11. |
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