Recombinant Human Casein Kinase I Isoform Alpha (CSNK1A1) Protein (His)

Name: Recombinant Human Casein Kinase I Isoform Alpha (CSNK1A1) Protein (His)
Brand: Beta LifeScience
SKU: BLC-10012P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) CSNK1A1.

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Product Overview

Description	Recombinant Human Casein Kinase I Isoform Alpha (CSNK1A1) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P48729
Target Symbol	CSNK1A1
Synonyms	Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer ; HLCDGP1; KC1A_HUMAN; PRO2975
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	ASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF
Expression Range	2-337aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.8kDa
Research Area	Cell Cycle
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.
Subcellular Location	Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, spindle.
Protein Families	Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
Database References	HGNC: 2451 OMIM: 600505 KEGG: hsa:1452 UniGene: PMID: 28945225 Data indicate that casein kinase 1 alpha (CK1alpha) RNA levels might serve as prognostic marker for colorectal cancer (CRC). PMID: 29409464 cellular kinase, casein kinase 1alpha (CK1alpha), is crucial for IAV HA-induced degradation of both IFNGR1 and IFNAR1. PMID: 29343571 casein kinase 1 phosphorylates the SQSTM1 S349 residue when harmful proteins accumulate under HSF1 stress PMID: 27846364 Maintenance of the progenitors thus requires cooperation by PRMT1 and CSNK1a1 to sustain proliferation gene expression and suppress premature differentiation driven by GRHL3 PMID: 28943242 These data indicate that CK1alpha has a dominant and non-redundant function in melanoma cells PMID: 27488834 Disruption of CK1alpha function in myeloma cells resulted in decreased Mdm2, increased p53 and p21 and reduced expression of beta-catenin and AKT. PMID: 28099937 CK1delta enhances EPO secretion from liver cancer cells under hypoxia by modifying HIF-2alpha and promoting its nuclear accumulation. PMID: 27686097 CKIalpha-mediated NS5A S235 phosphorylation is critical for HCV replication. CaMKII gamma and delta may have negative roles in the HCV life cycle. PMID: 27875595 Interactome analyses revealed that the Jade-1S mutant unable to be phosphorylated by CK1alpha has an increased binding affinity to proteins involved in chromatin remodelling, histone deacetylation, transcriptional repression, and ribosome biogenesis. PMID: 26919559 study focuses on, how the structural dynamics and conformational changes of two CK1 isoforms are synchronized in carcinogenic pathway PMID: 26788877 NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation. PMID: 26984280 The study describes the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1 alpha(CSNK1A1) using activity-based proteomics. PMID: 27031502 characterization of frequency and potential clinical impact of CSNK1A1 mutations in myelodysplastic syndrome(MDS) and acute myeloid leukemia following MDS in a large cohort of patients PMID: 25792355 The dynamical and conformational properties for each of three isoforms of CK1 are explored through molecular dynamics (MD) simulations. PMID: 25665722 Phosphorylation of LRRK2 by casein kinase 1alpha regulates trans-Golgi clustering via differential interaction with ARHGEF7. PMID: 25500533 Casein kinase 1alpha is a key negative regulator of oncogenic RAS-induced autophagy. Depletion or pharmacologic inhibition of CK1alpha enhanced autophagic flux in oncogenic RAS-driven human fibroblasts and multiple cancer cell lines. PMID: 25798617 CSNK1alpha1 has a role in mediating malignant plasma cell survival PMID: 24962017 Casein kinase 1 alpha specifically phosphorylates Jade-1 at an unprimed SLS phosphorylation site. PMID: 25100726 Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033 Data indicate that protein S phosphorylation by casein kinase 1 (CK1) and casein kinase 2 (CK2) increased activated proten C cofactor activity. PMID: 23580615 The substrate interacting residues in ck1alpha have been identified using the structural model of kinase - substrate peptide. The PKR interacting NS5A 1b residues have also been predicted. PMID: 23148689 DNA damage activates p53 in part by disrupting CK1a-MDMX interaction and reducing MDMX-p53 binding affinity. PMID: 23028042 identified casein kinase 1alpha (CK1alpha) as a direct target of miR-155 control which enhanced beta-catenin signaling and cyclin D1 expression, promoting tumor cell growth PMID: 22350414 We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131 mTOR cooperates with CK1alpha and betaTrCP to generate an auto-amplification loop to promote its own full activation. PMID: 22017877 The authors characterized the role of CKIalpha in the clock mechanism and found that CKIalpha-mediated phosphorylation stimulated degradation of a clock protein PER1, similar to the function of CKIdelta. PMID: 21179498 Our results indicate that the nuclear form of CK1alpha in humans, CK1alphaLS, plays a critical role in vascular cell proliferation, cellular activation, and hydrogen peroxide-mediated mitogenic signal transduction. PMID: 20696773 Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction. PMID: 20348946 Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio)are presented. PMID: 12210746 CK1alpha can promote cell survival by interfering with RXR agonist-induced apoptosis PMID: 15131121 CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C PMID: 15687492 NFAT1, a transcription factor connected with breast cancer metastasis, is activated by Wnt-5a through a Ca2+ signaling pathway in human breast epithelial cells which was simultaneously counteracted by a Wnt-5a-induced Yes/Cdc42 signaling pathway. PMID: 16880514 identification of the alpha isoform of casein kinase I as the cellular kinase important for the formation of hepatitis C virus (HCV) NS5A-p58; here we show the effects of this kinase on NS5A hyperphosphorylation and HCV replication PMID: 16943283 CK1alphaLS and hnRNP-C represent conserved components of a vertebrate-specific H(2)O(2)-responsive nuclear signaling pathway. PMID: 17626781 Studies suggest that CK1alpha is another kinase that regulates RIP1 function in NF-kappaB activation. PMID: 18067272 following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation. PMID: 18246126 Ser-155 and Ser-158 have important roles in the nucleocytoplasmic trafficking of OREBP/TonEBP and CK1 plays a major role in regulating this process PMID: 18411282 We show that the inhibition of CK1 activates RhoB and promotes RhoB dependent actin fiber formation and EGF-R level. PMID: 18590726 CK1 has a role as the Ser20 site kinase for p53 in DNA virus-infected cells PMID: 18669630 Phosphorylation of parkin by casein kinase I and cdk5 decreases parkin solubility, leading to its aggregation and inactivation. PMID: 19050041 'activated B-cell-like' subtype of diffuse large B-cell lymphoma cells required CK1alpha for constitutive NF-kappaB activity, indicating that CK1alpha functions as a conditionally essential malignancy gene PMID: 19118383 Casein Kinase 1 phosphorylates serine 56 of yellow fever virus methyltransferase. PMID: 19185594 Data show that Wnt-5a stimulation of breast epithelial cells leads to increased cell-cell adhesion, and that Wnt-5a/casein kinase Ialpha (CKIalpha)-specific phosphorylation of beta-catenin leads to increased complex formation of beta-catenin/E-cadherin. PMID: 19244247 CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability PMID: 19759023 Results suggest a role for mammalian and parasite casein kinase 1alpha in regulating IFNAR1 stability and type I IFN signaling. PMID: 19805514

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.