Recombinant Human Carbonic Anhydrase 12 (CA12) Protein (His-SUMO)

Name: Recombinant Human Carbonic Anhydrase 12 (CA12) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-04620P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Carbonic Anhydrase 12 (CA12) Protein (His-SUMO) is produced by our E.coli expression system. This is a extracellular protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O43570
Target Symbol	CA12
Synonyms	CA 12; CA XII; CA-XII; CA12; CAH12_HUMAN; Carbonate dehydratase XII; Carbonic anhydrase 12; Carbonic anhydrase XII; Carbonic dehydratase; CAXII; FLJ20151; HsT18816; T18816; Tumor antigen HOM RCC 3.1.3; Tumor antigen HOM-RCC-3.1.3
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	APVNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQVQVCTAAGLS
Expression Range	25-301aa
Protein Length	Extracellular Domain
Mol. Weight	47.1kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Reversible hydration of carbon dioxide.
Subcellular Location	Membrane; Single-pass type I membrane protein. Cell membrane.
Protein Families	Alpha-carbonic anhydrase family
Database References	HGNC: 1371 OMIM: 143860 KEGG: hsa:771 STRING: 9606.ENSP00000178638 UniGene: PMID: 26911677 The expression of HIF-1alpha, but not HIF-2alpha, decreased in degenerated disc samples and was positively correlated with carbonic anhydrase 12 expression. PMID: 26901836 mutation CA12(E143K) causes mouth dryness disease due to aberrant CA12 glycosylation. PMID: 26486891 Suggest CAXII as a new secondary marker of the multidrug resistance phenotype that influences Pgp activity directly. PMID: 25686827 an upregulation of CAXII in human T-cell acute lymphoblastic leukemia samples supporting the case that CAXII may represent a new therapeutic target for T-ALL/LL. PMID: 23348702 Serum CAXII levels were significantly higher in lung cancer patients than in healthy controls, providing evidence that CAXII may be a novel sero-diagnostic marker for lung cancer. PMID: 22439015 The expression of CA XII in oral squamous cell carcinoma (OSCC) samples can predict the progression of OSCC and survival of OSCC patients. PMID: 22172588 The development of acidosis with topiramate and zonisamide is not determined by drug dose or by treatment duration, but may be influenced by polymorphisms in the gene for CA type XII. PMID: 21278619 Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12.Data show that genes overexpressed in breast carcinoma including TFF1, TFF3, FOXA1 and CA12. PMID: 20132413 Study identified the association of a Glu143Lys mutation in carbonic anhydrase 12 (CA12) with the disease. PMID: 21184099 we demonstrate that the hyperchlohidrosis phenotype is due to a homozygous mutation in CA12, encoding carbonic anhydrase XII. PMID: 21035102 CA12 may be used as a novel prognostic marker in combination with histologic grade of invasive cervical cancer. PMID: 21040567 Carbonic anhydrase XII may affect the capability of invasion and migration of MDA-MB-231 cells, which may be mediated through the p38 MAPK pathway. PMID: 20434230 Suggest that CA XII should be considered a potential prognostic and therapeutic target in medulloblastomas and supratentorial primitive neuroectodermal tumours. PMID: 20398423 Differential modulation of the active site environment of human carbonic anhydrase XII by cationic quantum dots and polylysine. PMID: 20215053 The non-pigmented ciliary epithelial cells from glaucoma eyes expressed higher levels of CAXII, which may be a targeted gene in glaucoma. PMID: 12676895 CA XII showed no or weak immunoreaction in the normal gastric mucosa and was slightly increased in gastric tumors. PMID: 12854129 The interplay between the functional von Hippel-Lindau tumor suppressor and CA IX/CA XII in colorectal tumors seems rather PMID: 15849821 CA12 mRNA expression was detected in 88.1% of cervical cancers. PMID: 16416108 CA XII is commonly expressed in diffuse astrocytomas and that it might be used as a biomarker of poor prognosis. PMID: 18322268 Carbonic anhydrase (CA) IX (and possibly also CA XII) participates in pH regulation, which is important for survival of hypoxic cancer cells. Both enzymes are therefore promising targetable therapeutic molecules. [Review] PMID: 18336315 Crucial role of ER in the regulation of the CA12 gene. PMID: 18451179 In embryonic and early fetal tissues; there is no evidence of co-localization of CAIX and CAXII; CAIX and CAXII expression is closely related to cell origin and secretory activity involving proton transport, respectively. PMID: 19291313

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.