Recombinant Human Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO)

Name: Recombinant Human Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-06084P
Price: 418.4 USD
Availability: InStock

Collections: Antibody / cell therapy targets, Antibody therapeutic / adc target proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Calcitonin Gene-Related Peptide 1 (CALCA) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P06881
Target Symbol	CALCA
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	ACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAF
Expression Range	83-119aa
Protein Length	Partial
Mol. Weight	16.7 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.
Subcellular Location	Secreted.
Protein Families	Calcitonin family
Database References	HGNC: 1437 OMIM: 114130 UniGene: Hs.37058

Gene Functions References

CGRP expression in the infrapatellar fat pad of knee osteoarthritis patients is regulated by the COX-2/mPGES-1/PGE2 pathway. PMID: 30205824
PCT may not be sufficiently used as a sole marker of sepsis in neonates compared to CRP. PCT in conjunction with CRP and other tests for septic screen can aid in better diagnosis of neonatal sepsis. PMID: 29567889
Levels of CRP and PCT in CSF did not present greater accuracy in differentiating bacterial from viral meningitis compared to serum levels. PMID: 30143494
Medullary thyroid carcinoma (MTC) was histologically confirmed in all patients with positive procalcitonin (PCT) but not found in patients with negative PCT. Serum PCT levels were significantly higher in patients with MTC than in those without. PMID: 29635700
This is the largest data set evaluating PCT in neonatal and pediatric patients on extracorporeal membrane oxygenation (ECMO). A PCT value of 0.5 ng/mL had the most utility for determining the absence or presence of a bacterial infection in the setting of ECMO with a high sensitivity and negative predictive values. PMID: 29153041
The data shows a subgroup of patients 17/34 (50%) have either elevated PCT or CRP levels. This study is the first to report PCT values in antipsychotic drug-free patients with schizophrenia. PMID: 29723719
Procalcitonin is reliable in discriminating medullary thyroid carcinoma patients with active disease from those with no evidence of disease. PMID: 29707993
This retrospective cohort study aimed to identify the early postoperative kinetics of C-reactive protein (CRP) and procalcitonin (PCT) in children undergoing tetralogy of Fallot (ToF) correction. PMID: 29111402
PCT could be a promising marker of clinical deterioration to septic shock in acute cholangitis. Therefore, PCT might be used as a decision-supporting biomarker for urgent biliary decompression. PMID: 29480416
this study shows that pre-eclampsia affects procalcitonin production in placental tissue PMID: 29427369
peripheral concentration of procalcitonin (both immediate and average levels) might be an independent predictor for prognosis in myocardial infarction patients. PMID: 29179592
Patients with procalcitonin (PCT) above 0.25 mug/L are unlikely to have a unique diagnosis of flu. PMID: 28010128
The cut-off point of procalcitonin level for diagnosing sepsis in the meta-static solid tumor was higher compared to the standard value. PMID: 29382396
PCT is a useful marker for diagnosis of among catheter-related blood stream infection hemodialysis patients. PMID: 28777420
PCT is a useful diagnostic predictor of intra-abdominal infection after colorectal surgery. PMID: 29305753
It is unclear the receptor responsible by CGRP effects, as well as whether this neuropeptide acts directly or indirectly in liver, muscle and adipose tissue--{REVIEW} PMID: 28884411
The aim of the study was to define basal serum calcitonin (bCT) cut-off thresholds for diagnosing medullary thyroid cancer. PMID: 29169190
Serum levels of PCT and PSP are promising biomarkers for early diagnosis of pediatric acute osteomyelitis. PMID: 29091592
Procalcitonin and CRP serum levels are associated with the appearance of intra-abdominal infection after colorectal cancer surgery. PMID: 28918433
PCT distinguished sepsis and non-septic ICU patients. PMID: 28898742
Levels of procalcitonin are significantly elevated in subjects with diabetic foot ulcers and are correlated with clinical indices such as glycemic index and HbA1c level. PMID: 28736121
Serum procalcitonin can be used as a promising single biomarker, easily done in all setup with better accuracy. And it is comparable to computed tomography severity index and Ranson's scores in earlier prediction of severity of acute pancreatitis. PMID: 29446357
PCT showed statistically significant differences between the groups of septic and non-septic burn patients. PMID: 28454850
This study reports the transition dipole strengths and frequencies of the amyloid beta-sheet amide I mode for the aggregated proteins amyloid-beta1-40, calcitonin, alpha-synuclein, and glucagon. PMID: 28851219
Inhibition of SP through use of a neurokinin 1 (NK1) antagonist is highly beneficial following both traumatic brain injury (TBI) and ischaemic stroke in pre-clinical models. The role of CGRP is more unclear, especially with respect to TBI, with both elevations and reductions in CGRP levels reported following trauma PMID: 28817088
targeted non-alanine substitution analysis method and molecular modelling to the functionally important residues of extracellular loop 2 (ECL2) of the calcitonin gene related peptide (CGRP) receptor to reveal key molecular interactions. PMID: 28572046
Patients with lower gastrointestinal perforation (GIP) exhibited significantly higher APACHE II score, SOFA score and serum PCT level than patients with upper GIP. PMID: 28706425
PCT was an accurate predictor for the diagnosis of bacterial infection only in patients with acute liver failure unrelated to acetaminophen intoxication. PMID: 28272093
The maintenance of a high level of procalcitonin within 7 days after surgery and/or a second increase in procalcitonin between the fourth and the seventh postoperative day correlated with postoperative infection after pediatric cardiac surgery. PMID: 28266954
Systemic inflammation moderately occurs in the white coat hypertension. PCT monitoring may be a useful biomarker in inflammation related to atherosclerosis and early stage HT. PMID: 26040439
initial serum levels of procalcitonin might be used as an indicator for aggravation in patients with hepatobiliary tract infection at the emergency department, even though there is hemodynamic stability. PMID: 26096246
Serum procalcitonin levels are elevated in bacteremia. PMID: 28182347
Procalcitonin levels are an early indicator of complications in ICU patients with multiple trauma and patients undergoing major surgery. PMID: 28164563
These results establish that calcitonin gene-related peptide (CGRP) regulates HIV-1 degradation in Langerhans cells (LCs). As CGRP contributes to the sexual response and present within mucosal epithelia, HIV-1 proteasomal degradation in LCs might predominate in vivo and should be enhanced clinically. PMID: 28904199
Diagnostic value of sTREM-1, IL-8, PCT, and CRP in febrile neutropenia after autologous stem cell transplantation PMID: 28920169
PCT and CRP are reliable markers of infection and correlate with infection severity in children with liver disease. PMID: 26933799
The intrapatellar fat pad of knee osteoarthritis patients produces relatively high levels of CGRP. PMID: 28431586
CGRP and IL-4 positively regulated APN/CD13 expression and activity in psoriatic fibroblasts. PMID: 28387421
Elevated levels of procalcitonin in the early phase after pediatric cardiac surgery are associated with increased risk for major adverse events and postoperative renal failure and increased postoperative morbidity. PMID: 27124563
The results indicate a central role of neuroendocrine peptide aminoprocalcitonin (NPCT) in Alzheimer disease pathogenesis and suggest NPCT as a potential biomarker and therapeutic target. PMID: 27497681
Preoperative calcitonin levels were correlated with the presence of tumor, whereas alkaline phosphatase (ALP) levels were not. There were no significant associations between tumor volume and ALP or calcitonin levels in the preoperative or postoperative periods. During long-term follow-up, serum ALP was significantly associated with tumor recurrence, but serum calcitonin was not. PMID: 27922893
PCT median values were significantly higher in patients with sepsis than without during the first 10 postoperative days after cardiac surgery. PMID: 28550906
Point-of-care procalcitonin test can be used to substantially reduce antibiotic exposure in patients hospitalized with acute exacerbation of COPD. PMID: 27382274
Data suggest CGRP receptor (CGRPR) ECL2 (extracellular loop 2 domain) enables interaction with N-terminal residues of CGRP; this provides evidence for dual involvement of ECL2 in two-domain binding model of CGRP/CGRPR interaction; CGRPR is obligate heterodimer of CLR/RAMP1. (CGRP = calcitonin gene-related peptide; CLR = calcitonin receptor-like receptor; RAMP1 = receptor [calcitonin] activity modifying protein 1) PMID: 28691801
Elevated Expression of PCT is associated with Sepsis. PMID: 28005932
Serum procalcitonin levels were not increased in patients with ankylosing spondylitis. PMID: 24798190
Both procalcitonin levels and white blood cell counts were significantly higher in patients with postoperative intraabdominal infections (IAIs) than those in patients without IAIs on postoperative day (POD) 1, POD 3, and POD 5, whereas C-reactive protein levels differed significantly on POD 3 and POD 5. PMID: 27884320
High Procalcitonin expression is associated with engraftment syndrome. PMID: 27762068
In mammalian neuromuscular junctions, different isoforms of exogenously applied calcitonin gene-related peptide uniformly potentiate amplitudes of evoked and spontaneous postsynaptic potentials acting presynaptically via an increase in acetylcholine quantal size. PMID: 27288020
Mouse and human heart valves expressed mRNAs for the CRL ligands adrenomedullin (AM), adrenomedullin-2 (AM-2) and calcitonin gene-related peptide (CGRP) and for their receptor components, i.e., CRL and receptor-activity-modifying proteins 1-3. PMID: 27553639

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.