Recombinant Human C5A Anaphylatoxin Chemotactic Receptor 1 (C5AR1) Protein (His), Active

Name: Recombinant Human C5A Anaphylatoxin Chemotactic Receptor 1 (C5AR1) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05774P
Availability: InStock

this product is detected by Mouse anti-6*His monoclonal antibody.

Activity Measured by its binding ability in a functional ELISA. Immobilized human C5AR1 at 10 μg/ml can bind Anti-C5AR1 recombinant antibody , the EC 50 is 1.239-3.760 ng/mL. Biological Activity Assay

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Product Overview

Description	Recombinant Human C5A Anaphylatoxin Chemotactic Receptor 1 (C5AR1) Protein (His), Active is produced by our Mammalian cell expression system. This is a full length protein.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized human C5AR1 at 10 μg/mL can bind Anti-C5AR1 recombinant antibody , the EC 50 is 1.239-3.760 ng/mL.
Uniprotkb	P21730
Target Symbol	C5AR1
Synonyms	C5AR1; C5AR; C5R1; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin chemotactic receptor; C5a-R; C5aR; CD antigen CD88
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	C-10His
Target Protein Sequence	MDSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRVPDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTINAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFGGAACSILPSLILLNMYASILLLATISADRFLLVFKPIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYRVVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLGFLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVVAVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLKKLDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSLPSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV
Expression Range	1-350aa
Protein Length	Full Length
Mol. Weight	41.1 kDa
Form	Liquid
Buffer	PBS, pH 7.4, 6% trehalose
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production.
Subcellular Location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle.
Protein Families	G-protein coupled receptor 1 family
Database References	HGNC: 1338 OMIM: 113995 KEGG: hsa:728 STRING: 9606.ENSP00000347197 UniGene: PMID: 27539194 X ray structure of a thermostabilized C5aR1 (known as C5aR1 StaR) in complex with NDT9513727 PMID: 29300009 this study shows that C5a receptor (CD88) promotes motility and invasiveness of gastric cancer by activating RhoA PMID: 27756879 Serum containing active complement enhanced pneumococcal induced proinflammatory cytokine production through C5a release and C5aR crosstalk. PMID: 27721145 C5aR plays a role in T helper cell polarization induced by Mycobacterium tuberculosis and this effect is strain- and donor-dependent. PMID: 28237029 Panton-Valentine leucotoxin LukS-PV/LukF-PV and the gamma-hemolysin HlgC/HlgB, bind the C5a complement-derived peptide receptor, inducing intracellular calcium release in neutrophils. PMID: 28117704 TLR4 and C5aR crosstalk in dendritic cells induces a core regulatory network of RSK2, PI3Kbeta, SGK1, and FOXO transcription factors. PMID: 28733463 Results showed that 7-oxygenated cholesterol derivatives have differential effects on monocyte/macrophage expression of IL-8 and C5a receptor and that C5a receptor is involved in 7alphaOHChol-induced IL-8 expression via PI3K and MEK. Study demonstrated expression of IL-8 and C5a receptor primarily by 7alpha-hydroxycholesterol in monocytes/macrophages. PMID: 28323848 Reducing RPS19 in tumor cells or blocking the C5a receptor 1-RPS19 interaction decreases RPS19-mediated immunosuppression, impairs tumor growth, and delays the development of tumors in a transgenic model of breast cancer PMID: 28228558 C5aR expression in gastric cancer was associated with cancer progression, liver metastasis, and poor prognosis. PMID: 27688239 this study shows that downregulation of CD88 after stimulation with IL-8 is more pronounced in adults than in neonates, whereas fMLP induces changes in receptor expression that are of the same magnitude in neutrophils from neonates as from adults PMID: 27606963 Relation of C5aR expression to tumor development and poor prognosis of the breast cancer patients PMID: 26494574 The objective of the present study was to validate a receptor occupancy (RO) assay for a human anti-C5aR monoclonal antibody drug candidate, NNC0215-0384 (NN0384) PMID: 26084468 These data thus suggest that C5aR1 acts as an enhancer of CCR5-mediated HIV entry into macrophages, the targeting of which may prove useful to reduce HIV infection by R5 strains. PMID: 26537334 C5AR and C5L2-mediated neutrophil dysfunction is associated with a poor outcome in sepsis. PMID: 25726869 that C5aR stimulates cell invasion and migration via ERK1/2-mediated epithelial-mesenchymal transition in hepatocellular carcinoma cells PMID: 26475622 Inhibition of C5aR1 leads to a significant reduction of CD66b and CD11b expression indicating a lower neutrophil activation status and block of granule exocytosis. PMID: 26176669 that rs10853784 of C5aR1 gene are associated with coronary artery disease in Han population of China PMID: 25924896 96.7% of the metastatic RCCs had C5aR expression, vs. 50.5% of the non-metastatic ones. C5a triggered ERK- and PI3Kdependent invasion of the C5aR-expressing renal carcinoma cells. The C5a-C5aR axis plays a crucial role in renal carcinoma cell invasion. PMID: 25682807 These results illustrate the novel activity of the C5a-C5aR axis that promotes human NPC cell proliferation through PCAFmediated STAT3 acetylation. PMID: 25174320 C5a/C5aR1 signalling plays a key role in disrupting blood-brain barrier integrity through different cascades including NF-KappaB translocation leading to altered tight junction proteins, Claudin-5 and ZO-1, and actin reorganization in the lupus setting PMID: 26059553 ). Low frequency of the C5aR 450 CT genotype, which apparently is a feature of certain kidney diseases, appears to be associated with good graft outcome in kidney transplantation. PMID: 25582053 C5AR1 SNP -1330T/G may serve as a useful pharmacodynamic predictor of nonsedating H1-antihistamine efficacy in chronic spontaneous urticaria (CSU), and -1330T alleles may be taken as a risk factor for CSU. PMID: 25455139 Porphyromonas gingivalis induces C5aR-TLR2 coassociation in neutrophils. PMID: 24922578 inhibition of TAFIa activity, however, was substrate-specific, because neither mAb inhibited the cleavage of thrombin-activated osteopontin and C5a by TAFIa, thus sparing the anti-inflammatory activity of TAFIa PMID: 24134522 C5aR and C5L2 may have roles in adiposity in women PMID: 24523571 The uPAR-C5aR axis, via the underlying NFkappaB transcriptional program, controls osteogenic differentiation with functional impact on vascular calcification in vivo. PMID: 24192237 High levels of CD88 are associated with poor prognosis of NSCLC. PMID: 23706417 C5aR cleavage is induced by neutrophil serine protease. The consequent impairment of C5aR activity contributes to suboptimal local neutrophil priming and bacterial clearance. PMID: 24446515 complement can regulate Muller cells through C5aR, which may contribute to the pathogenesis of retinal diseases, including DR. PMID: 24265019 C5aR is a regulatory switch that modulates TLR4 signaling via the Galphai/c-Raf/MEK/ERK signaling axis in human macrophages but not monocytes. PMID: 24043889 The C5aR binding Panton-Valentine Leukocidin component, LukS-PV, is a potent inhibitor of C5a-induced immune cell activation. PMID: 23684309 Data indicate that C5aR was aberrantly expressed in various cancers. PMID: 23287562 CD88 receptor expression is increased in brain from Alzheimer's disease patients compared to controls or vascular dementia patients. PMID: 23394121 C5aR-C5L2 homodimerization versus heterodimerization contribute to differential responses obtained following ASP vs C5a stimulation. PMID: 23268185 An RP S19 oligomer inhibitor and a C5aR inhibitor blocked the association of the K562 basophilic EB-like cells. PMID: 23346183 C5aR is shed during sepsis, resulting in the appearance of a previously undetected circulating C5aR in the serum of patients that negatively correlates with survival. PMID: 23479227 the neutrophil C5aR acts as a dual pro-inflammatory and pro-apoptosis receptor during the initiation and the resolution phases of acute inflammation, respectively--{review} PMID: 23402022 C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a PMID: 23041570 C5aR was expressed in the thick ascending limb of Henle's loop and first part of the distal convoluted tubule. Under inflammatory conditions, C5aR was de novo expressed in proximal tubuli. PMID: 22960554 Complement C5a upregulates prolabor mediators in human gestational tissues via CD88-mediated NFKB activation. PMID: 22441801 Analysis of the 3'UTR of the C5aR mRNA showed that it contained two AU-rich elements (AREs), however site directed mutagenesis showed that these had no effect on basal expression. PMID: 22608366 The aim of this study was to investigate the genetic alterations and mRNA expression pattern of C5aR and C5L2 genes in neutrophils from attack-free familial Mediterranean fever patients. PMID: 22187344 the monocyte C5aR selectively activates the classical pathway with the binding of C5a and the alternative pathway with the binding of C5a/RP S19. PMID: 21613290 C5a, a complement activation product, is a useful marker in predicting the severity of necrotizing enterocolitis. PMID: 21628869 Among a cohort of 60 critically ill patients, C5a-mediated neutrophil dysfunction (as determined by reduced CD88 expression) was a strong predictor for subsequent acquisition of nosocomial infection PMID: 21292772 Data show that when a Gi/PI3K pathway is partially blocked, C5a receptors stimulate an alternative p38MAPK pathway. PMID: 20473571 expression profiles of CRegs and CD88 on leukocytes are specifically altered after polytrauma in humans, indicating a trauma-induced "complementopathy PMID: 19864971 The elevated local and systemic C5a levels, and additional individual local C5a increase during the exacerbation support the importance of C5a in Chronic Obstructive Pulmonary Disease. PMID: 20500690 Data show that C5L2 is predominantly intracellular, while C5aR is expressed on the plasma membrane, and that internalized C5aR following ligand binding is co-localized with both C5L2 and beta-arrestin. PMID: 20044484

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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