Recombinant Human Bone Morphogenetic Protein 7 (BMP7) Protein (GST)

Name: Recombinant Human Bone Morphogenetic Protein 7 (BMP7) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-03597P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Human Bone Morphogenetic Protein 7 (BMP7) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P18075
Target Symbol	BMP7
Synonyms	BMP 7; BMP-7; Bmp7; BMP7_HUMAN; Bone morphogenetic protein 7; Bone morphogenic protein 7; Eptotermin alfa ; OP 1; OP-1; OP1 ; Osteogenic protein 1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	STGSKQRSQNRSKTPKNQEALRMANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH
Expression Range	293-431aa
Protein Length	Full Length of Mature Protein
Mol. Weight	42.7kDa
Research Area	Developmental Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Growth factor of the TGF-beta superfamily that plays important role in various biological processes, including embryogenesis, hematopoiesis, neurogenesis and skeletal morphogenesis. Initiates the canonical BMP signaling cascade by associating with type I receptor ACVR1 and type II receptor ACVR2A. Once all three components are bound together in a complex at the cell surface, ACVR2A phosphorylates and activates ACVR1. In turn, ACVR1 propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. For specific functions such as growth cone collapse in developing spinal neurons and chemotaxis of monocytes, uses also BMPR2 as type II receptor. Can also signal through non-canonical pathways such as P38 MAP kinase signaling cascade that promotes brown adipocyte differentiation through activation of target genes, including members of the SOX family of transcription factors.
Subcellular Location	Secreted.
Protein Families	TGF-beta family
Database References	HGNC: 1074 OMIM: 112267 KEGG: hsa:655 STRING: 9606.ENSP00000379204 UniGene: PMID: 30015928 in this study, the influence of IGF-1 and BMP-7 in different concentrations on the osteogenic differentiation of two human MSC-subtypes, isolated from reaming debris (RMSC) and iliac crest bone marrow (BMSC) has been assessed PMID: 29874864 BMP7 contributes to epithelial-to-mesenchymal transition-like responses and plays a role equivalent to TGF-β in the course of corneal wound healing. PMID: 29747422 On the contrary, BMP7 specific antibody inhibits the HNK-induced activation of p53 in colon cancer cells and partly decreases the total level of p53. Our findings suggested that HNK may be a promising anticancer drug for CRC; activation of p53 plays an important role in the anticancer activity of HNK, which may be initialized partly by the HNK-induced upregulation of BMP7. PMID: 28731124 All isoforms of type I and type II BMP receptors were expressed in both Ca9-22 and HSC3 cells and BMP7 stimulation resulted in the phosphorylation of Smad1/5/8 in both cell lines PMID: 28812969 the results of the present study indicate that BMP7 may inhibit excessive scar formation via activation of the BMP7/Smad1/5/8 signaling pathway. PMID: 28627680 BMP7, in particular combined with MSC, seems to have a favourable application also in periodontal regeneration. PMID: 28735626 Implantation of morphogenetic protein-7 (BMP-7) gene activated fat tissue fragments can elicit regeneration of large bone defects in rats and could become a clinically expeditious strategy for in vivo bone tissue engineering. PMID: 27373764 the present results showed that OP-1 might serve as a biochemical parameter for determining disease severity in primary knee Osteoarthritis (OA). Further studies with larger sample size need to be carried out to confirm OP-1 as a marker of disease status. Studies are required to be done to examine the genetic and lifestyle factors that may contribute to the development of knee OA PMID: 28474632 BMP7-Based Functionalized Self-Assembling Peptides Protect Nucleus Pulposus-Derived Stem Cells From Apoptosis In Vitro PMID: 27582519 results showed that the expression of cartilage-associated markers in ESC-MSCs induced by the TGFbeta1 and BMP7 combination was increased compared to induction with TGFbeta1 alone. The TGFbeta1 and BMP7 combination upregulated the expression of TGFbeta receptor and the production of endogenous TGFbetas compared to TGFbeta1 induction. PMID: 27292615 Overexpression of truncated ALK5 in a B-cell line counteracted BMP-7-induced apoptosis, whereas overexpression of truncated ALK4 had no effect. PMID: 28489883 study suggests that the common genetic polymorphisms of BMP7 gene are not major contributors to variations in Bone Mineral Density or osteoporotic fracture in postmenopausal Chinese women. PMID: 27264311 Patients with hereditary pulmonary arterial hypertension had significantly higher BMP7 concentrations than patients with idiopathic pulmonary arterial hypertension and control subjects. PMID: 27001265 Expression of bone morphogenetic protein 7 ligand was significantly increased in patients with abnormal uterine bleeding. Study demonstrates that bone morphogenetic protein 7 is a promising target for future investigation and pharmacologic treatment of abnormal uterine bleeding. PMID: 28142396 BMP-7 directly upregulates adhesion and migration of human monocytic cells via activation of beta 2 integrins, Akt, and FAK. PMID: 27669124 results showed that stimulation by BMP-7 leads to an increased osteogenic potential of Mesenchymal stem cells. PMID: 28009419 The disequilibrium between BMP-7 and TGF-beta signals plays a relevant role in the LV remodelling response to haemodynamic stress in aortic stenosis patients with left ventricular hypertrophy/dysfunction. PMID: 27068510 BMP7 may partly mediate the antiproliferative effect of oridonin by activating p38 MAPK in colon cancer cells. PMID: 26986967 Data show that interaction of fibrillin-1 with the bone morphogenetic protein 7 (BMP-7) complex results in a conformational change. PMID: 27059954 the role of BMP-7 and its downstream signals in the neuroprotective effects of oxygen-glucose deprivation preconditioning PMID: 26385023 miR-137/BMP7 axis could contribute to the progression of non-small cell lung cancer. PMID: 26617798 Inhibition of BMP7 expression mediated by KDM5C promotes neoplasm invasion in hepatocellular carcinoma cells. PMID: 26503415 Adenovirus-mediated FoxC2 expression enhances BMP7-facilitated anabolism in nucleus pulposus cells of the intervertebral discs. PMID: 26824865 ANGPTL4 might promote metastasis and might inhibit apoptosis of colorectal cancer cells by up-regulation of BMP7. PMID: 26417691 MiR-22 promotes the development of liver cirrhosis through BMP7 suppression. PMID: 26112332 Transfection of HOXA13 in HKCs could inhibit the degree of EMT induced by albumin-overload, possibly by increasing BMP-7 expression. PMID: 26695677 rs6127973 may be a risk factor of being Nonsyndromic orofacial clefts and confirmed the role of BMP7 gene in orofacial deformity from Western Han Chinese PMID: 25662552 OP-1 levels detected by a polyclonal antibody were found to be much higher than the reference range and correlated positively with the severity of osteoarthritis PMID: 25091889 Exposure of adipogenic stem cells to BMP7 promoted gene expression signatures of brown adipocytes. PMID: 25534037 BMP-7 improves proliferation and hematopoietic reconstitution potential of ex vivo expanded cord blood-derived CD34(+). cells.( PMID: 25192984 hyaluronan regulates bone morphogenetic protein-7-dependent prevention and reversal of myofibroblast phenotype PMID: 25716319 Bone morphogenetic protein 7 increased vascular endothelial growth factor-a expression in human granulosa cells and VEGF receptor expression in endothelial cells to stimulate angiogenesis. PMID: 24023033 Study highlights the potential importance of RGMb in propagating pro-angiogenic effects of HGF and BMP-7. PMID: 24970050 BMP-7 may play anti-inflammatory and anti-fibrogenic roles in the pathogenesis of chronic hepatitis B infection. PMID: 24820464 BMP-7 inhibits TGF-beta-induced cholangiocarcinoma cell migration through inhibition of TGF-beta-mediated Twist and N-cadherin expression PMID: 24969562 BMP-7 represses albumin-induced chemokine synthesis in kidney tubular epithelial cells through destabilization of NF-kappaB-inducing kinase. PMID: 24418819 BMP7 expression correlates with secondary drug resistance in mantle cell lymphoma. PMID: 24069261 Expression and purification of recombinant human bone morphogenetic protein-7 in Escherichia coli. PMID: 24117149 These results suggested an association between a BMP7 single nucleotide polymorphism and selective serotonin reuptake inhibitors treatment response. PMID: 23697978 These findings indicate that BMP-7 mediates ischemic preconditioning -induced tolerance to cerebral ischemia/reperfusion, probably through inhibition of apoptosis. PMID: 24184479 BMP7 induces Langerhans cell differentiation and proliferation by activating the BMP type-I receptor ALK3 in the absence of canonical TGF-beta1-ALK5 signaling. PMID: 24190429 summarize recent knowledge of BMP-7 function and discuss attempts to use this cytokine as a drug to reverse TGF-beta-induced fibrogenesis PMID: 23747893 Exogenous BMP-7 significantly decreased the degree of hepatic fibrosis in both the acute and chronic stages of hepato-schistosomiasis, and the regulatory mechanism may involve the TGF-beta/Smad signaling pathway. PMID: 23539268 The anti-fibrogenic mechanism of rhBMP-7 in the rat liver fibrosis was depended on the reduction of TGF-b1 overexpression and the inhibition of TGF-b1 triggered intracellular signalling in hepatic cells. PMID: 23471555 Nucleus pulposus cells expressing hBMP7 can prevent the degeneration of allogenic intervertebral disc degeneration in a canine transplantation model. PMID: 23580474 BMP7 modulates the expression of E-cadherin and MMP-9, and by which mechanism it may regulate cell migration and metastasis of esophageal squamous cell carcinoma. PMID: 23504348 A novel human bone morphogenetic protein-7 variant with an enriched heparin-binding site PMID: 23888776 There is efficient and reproducible induction of SZP/lubricin accumulation by TGF-beta1 and BMP-7 in skeletal muscle-derived mesenchymal stem cells. PMID: 22490392 monoubiquitinated SMAD6 impairs the binding affinity of non-modified SMAD6 to the BMP type I receptor. Moreover, UBE2O and SMAD6 cooperated in the regulation of BMP7-induced adipogenesis PMID: 23455153

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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