Recombinant Human BID Protein

Name: Recombinant Human BID Protein
Brand: Beta LifeScience
SKU: BL-2036NP
Availability: InStock

BL-2036NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human BH3-Interacting Domain Death Agonist is produced by our E.coli expression system and the target gene encoding Met1-Asp195 is expressed.
Accession	P55957
Synonym	BH3-Interacting Domain Death Agonist; p22 BID; BID
Gene Background	BH3-Interacting Domain Death Agonist (BID) is a member of the Bcl-2 protein family which regulates outer mitochondrial membrane permeability. BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. Interaction of Bid with Bak causes altered mitochondrial membrane permeability. BID contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. It is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent, and also offer a new strategy for the treatment of acute renal failure associated with ischemia-reperfusion. BID receives direct inputs from a key regulator of the cell cycle arrest/DNA repair machinery (ATM), and therefore is an excellent candidate to coordinate genotoxic stress responses and apoptotic cell death. BID is a novel pro-apoptosis Bcl-2 family protein that is activated by caspase 8 in response to Fas/TNF-R1 death receptor signals. Deletion of BID inhibits carcinogenesis in the liver, although this genetic alteration promotes tumorigenesis in the myeloid cells. This is likely related to the function of BID to promote cell cycle progression into S phase. BID could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint.
Molecular Mass	21.99 KDa
Apmol Mass	20 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM KCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Induces caspases and apoptosis. Counters the protective effect of BCL2.; Induces caspase activation and apoptosis. Allows the release of cytochrome c.; Induces ICE-like proteases and apoptosis.; Induces ICE-like proteases and apoptosis.; Does not induce apoptosis.; Induces ICE-like proteases and apoptosis.
Subcellular Location	Cytoplasm. Mitochondrion membrane. Mitochondrion outer membrane.; [BH3-interacting domain death agonist p15]: Mitochondrion membrane.; [BH3-interacting domain death agonist p13]: Mitochondrion membrane.; [Isoform 1]: Cytoplasm.; [Isoform 3]: Cytoplasm.; [Isoform 2]: Mitochondrion membrane.
Database References	HGNC: 1050 OMIM: 601997 KEGG: hsa:637 STRING: 9606.ENSP00000318822 UniGene: Hs.591054
Tissue Specificity	[Isoform 2]: Expressed in spleen, pancreas and placenta (at protein level).; [Isoform 3]: Expressed in lung, pancreas and spleen (at protein level).; [Isoform 4]: Expressed in lung and pancreas (at protein level).

Gene Functions References

results suggest that histone modifications in the promoter region of miR-500a may be responsible for the increased expression of miR-500a in Hepatocellular carcinoma (HCC), which promotes cancer progression by targeting BID, indicating that miR-500a may be a potential prognostic predictor and therapeutic target for HCC patients. PMID: 29969781
The caspase-8/Bid/cytochrome c axis links signals from death receptors to mitochondrial reactive oxygen species production. PMID: 28888620
administration of SMAC or BH3 mimetics following short-term paclitaxel treatment could be an effective therapeutic strategy for TNBC, while only BH3-mimetics could effectively overcome long-term paclitaxel resistance PMID: 28187446
caspase-10 was dispensable for enhancement of cisplatin/LA-12 and TRAIL combination-induced cell death and stimulation of Bid cleavage PMID: 29182622
an oligomeric arrangement of Bid, Bax, and possibly other members of the Bcl-2 family of proteins that form a self-propagating network that permeabilizes the outer-mitochondrial membrane. PMID: 27763642
Combined therapy with Seliciclib((R)) and Belinostat((R)) results in eradication of non-small cell lung cancer via apoptosis induction and BID activation. PMID: 27461583
Results from genome-wide DNA methylation, functional network analysis and pyrosequencing, show selective CpG sites (NOS1AP, BID, and GABRB1) differentially methylated in smokers and chronic obstructive pulmonary disease patients compared to nonsmokers. PMID: 28416970
The adenovirus-mediated truncated Bid overexpression induced by the Cre/LoxP system can effectively eliminate CD133+ ovarian cancer stem cells, representing a novel therapeutic strategy for the treatment of ovarian cancer. PMID: 27878291
MiR-20a-directed regulation of BID in colorectal cancer. PMID: 28004114
the results establish that cleavage by caspase 8 and the subsequent association with the outer mitochondrial membrane are two critical events that activate Bid during death receptor-mediated apoptosis. PMID: 27053107
The results indicate that BID-independent pathways are responsible for FAS-dependent human islet cell death in Type 1 diabetes. PMID: 26758067
The BID-MTCH2 axis regulates the differentiation/apoptosis of stem cells and mitochondrial metabolism. (Review) PMID: 26827940
Data suggest, in models of spatial propagation of mitochondrial permeabilization during apoptosis, there appears to be requirement for cooperative signaling involving truncated-BID and ROS (reactive oxygen species) for efficient/ robust propagation. PMID: 26699404
this study emphasizes that the coordinated action of hGzmB-activated p53 and GzmB-cleaved Bid is important for GzmB-induced cell death and for cytotoxic lymphocyte/Natural Killer Cell-mediated killing of target cells. PMID: 25404359
Tax confers apoptosis resistance to HTLV-1-infected T cells by suppressing the expression of Bim and Bid. PMID: 25522269
EG also activated the death receptor-dependent pathway of apoptosis by enhancing the expression of caspases-8, -9, and -3 and the Bcl-2 interacting domain (Bid). PMID: 23109891
Inhibition of NANOGP8 or NANOG enhances the cytotoxicity of BH3 mimetics. PMID: 25208882
JNK1/2 regulate Bid by direct phosphorylation at Thr59. PMID: 25077544
Association of BID gene polymorphisms with proteinuria of immunoglobulin A nephropathy PMID: 24621205
The extrinsic apoptotic pathway and BID are activated in adenomas from NSAID-treated patients. NSAIDS only activate BID in cells with APC deficiency and ensuing c-Myc activation. PMID: 25368155
Gli1 has a role in regulating the S-phase checkpoint in tumor cells via Bid protein, and its inhibition sensitizes to DNA topoisomerase 1 inhibitors PMID: 25253693
Bid is phosphorylated during mitosis within its regulatory loop and sensitizes mitochondria for mitochondrial outer membrane permeabilization if mitotic exit is delayed. PMID: 24767991
BID expression was found in 53.6% of gastric cancer patients. PMID: 24741635
BID is associated with ossification of the posterior longitudinal ligament , and both the G allele of a missense SNP (rs8190315, Ser10Gly) and C allele of a synonymous SNP (rs2072392, Asp60Asp) are risk factors for the development of ossification PMID: 24398548
Reduced Bid expression is associated with endometrial cancer. PMID: 24645842
FTY720 induces apoptosis of chronic myelogenous leukemia cells via dual activation of BIM and BID and overcomes various types of resistance to tyrosine kinase inhibitors. PMID: 23851982
structural details on the membrane-associated state of tBid and the functional implications of its membrane-associated BH3 domain PMID: 24158446
GrH induced cell death via a Bcl-2-sensitive mitochondrial pathway without direct processing of Bid. PMID: 23352961
Caspase-8 binding to cardiolipin in giant unilamellar vesicles provides a functional docking platform for bid PMID: 23418437
Three cathepsin D-specific cleavage sites in Bid, Phe24, Trp48, and Phe183, were identified. PMID: 22964611
Patients with Parkinson disease have an activated Bid-mediated destructive signal pathway via tumor necrosis factor death receptor I (TNFRI) in the temporal cortex. PMID: 23019260
Molecular basis of the interaction between proapoptotic truncated BID (tBID) protein and mitochondrial carrier homologue 2 (MTCH2) protein PMID: 22416135
Results suggest that truncated BID specifically interacts with phosphatidylserine/cardiolipin and decreases membrane integrity without the aid of other pro-apoptotic proteins. PMID: 22189507
14-3-3 theta/tau and tBID have roles as predictive biomarkers of neoadjuvant chemotherapy resistance in breast cancer PMID: 22115752
Decreased apoptosis and expression of Bid, increased level of Bcl-Xl may play some important roles in human jejunal stromal tumors, and Bcl-Xl/Bid ratio may be as a new potentially associated index. PMID: 22339673
Bid engages a ROS-dependent, local intermitochondrial potentiation mechanism that amplifies the apoptotic signal as a wave PMID: 22393005
critical for controlling cell viability regulated by IFN-alpha in ovarian adenocarcinoma cells PMID: 22130162
chronic ethanol increases CYP2E1 activity in adipose, leading to Bid-mediated apoptosis and activation of complement via C1q PMID: 21856753
show that due to the high affinity of BAX for BCL-2, BCL-w and A1, and of BAK for BCL-X(L), MCL-1 and A1, only a subset of BH3-only proteins, including BID, could be expected to free BAX or BAK from the antiapoptotic BCL-2 proteins to elicit apoptosis PMID: 21060336
a direct role for the BH3 interacting domain death agonist acting at the level of the DNA damage sensor complex to amplify the Atr-directed cellular response to replicative damage PMID: 21113148
Data show that the expression of apoptosis proteins caspase-3,-8,-9, and Bid in the RNP of the disc and the SNP was different in each patient. PMID: 21178828
Cells stabilize active caspase-8 on the mitochondria in order to specifically target mitochondria-associated BID. PMID: 21072056
When tBid was introduced into the HIV-1 LTR-based, Tat- and Rev-dependent transgene expression vector pLRed(INS)2R, very efficient induction of apoptosis was observed within 24 hours,but only in the presence of both HIV-1 regulatory proteins Tat and Rev. PMID: 21223573
tBID and other BCL-2 proteins have a role in osteosarcoma [commentary] PMID: 19029793
In rheumatoid arthritis fibroblast like synovial cells phosphorylation of Akt protects against Fas-induced apoptosis through inhibition of Bid cleavage. PMID: 20187936
The interface between apoptosis initiation and execution by determining caspase-8 activation, Bid cleavage and mitochondrial engagement (onset of mitochondrial depolarisation) in individual HeLa, was analysed. PMID: 20356928
Data revealed that the level of tBid expression in breast cancer cells were nearly 11 times more than normal cells because of the cancer specific promoters. PMID: 19918914
Bid may serve as a critical integrating factor of the death receptor and mitochondrial pathway in alpha-tocopheryl succinate-mediated apoptosis. PMID: 19640637
tBid-induced permeabilization of the outer membrane permits release of cytochrome c and Smac/DIABLO from all domains of intermembrane space. tBid-induced loss of DeltaPsi(m) occurs after cytochrome c release and reflects impairment of oxidative metabolism PMID: 11741882
Cytochrome c release upon Fas receptor activation on translocation of full-length bid PMID: 11790791

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