Recombinant Human Beta-Catenin-Interacting Protein 1 (CTNNBIP1) Protein (GST)

Name: Recombinant Human Beta-Catenin-Interacting Protein 1 (CTNNBIP1) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-08702P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Beta-Catenin-Interacting Protein 1 (CTNNBIP1) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9NSA3
Target Symbol	CTNNBIP1
Synonyms	Beta catenin interacting protein 1; Beta catenin interacting protein ICAT; Beta-catenin-interacting protein 1; Catenin beta interacting protein 1; CNBP1_HUMAN; CTNNBIP 1; CTNNBIP1; ICAT; Inhibitor of beta catenin and Tcf 4; Inhibitor of beta-catenin and Tcf-4; MGC15093
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	MNREGAPGKSPEEMYIQQKVRVLLMLRKMGSNLTASEEEFLRTYAGVVNSQLSQLPPHSIDQGAEDVVMAFSRSETEDRRQ
Expression Range	1-81aa
Protein Length	Full Length
Mol. Weight	36.2kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Prevents the interaction between CTNNB1 and TCF family members, and acts as negative regulator of the Wnt signaling pathway.
Subcellular Location	Cytoplasm. Nucleus.
Protein Families	CTNNBIP1 family
Database References	HGNC: 16913 OMIM: 607758 KEGG: hsa:56998 STRING: 9606.ENSP00000366466 UniGene: PMID: 29048651 Low PLD1 expression and high ICAT expression were significantly associated with increased survival in colorectal cancer patients. PMID: 28939743 The present work aims to investigate the relationship between the expression of AEG-1(astrocyte elevated gene-1), b-FGF(basic-fibroblast growth factor), beta-catenin, Ki-67, TNF-alpha (tumor necrosis factor-alfa) other prognostic parameters in DC (Ductal Carcinomas) and ductal intraepithelial neoplasm. We found a relationship between these factors. PMID: 26096243 Overexpression of ICAT promoted Caski cells' proliferation, arrested the cell cycle in the S phase and enhanced cell migration. Conclusion Overexpression of ICAT can promote the proliferation and migration of Caski cervical cancer cells. PMID: 27774943 Somatic mutation of beta-catenin (CTNNB1) is known to be crucial for Wilms tumor development in up to 15% of cases. PMID: 27679509 CTNNBIP1 expression correlated with longer overall survival in LAC patients. This study reveals that miR-214 plays a critical role in CSLC self-renewal and stemness by targeting CTNNBIP1. PMID: 26299367 Data show that microRNA miR-215 activates beta-catenin pathways by decreasing catenin beta interacting protein 1 (CTNNBIP1)expression in gliomas. PMID: 26317904 Simultaneous silencing of beta-catenin and STAT3 synergistically induces apoptosis and inhibits cell proliferation in HepG2 liver cancer cells. PMID: 25845340 Finally, pro-incubation with idebenone inhibited mitochondrial dysfunction induced by oxLDL through the mitochondrial-dependent apoptotic pathway and GSK3beta/beta-catenin signalling pathways. PMID: 26284974 A potent beta-catenin inhibitor, ICAT/CTNNBIP1 was a direct target of miR-424-5p. PMID: 25175916 Particulate matter (PM10) downregulates E-Cadherin/beta-Catenin expression. PMID: 26047787 A statistically significant lapatinib- and gefitinib-induced repression of cyclin D1, MMP9 and beta-catenin in CERV196 cells. PMID: 26124325 Nuclear YAP and cytoplasmic beta-catenin play important roles in carcinogenesis. PMID: 26124339 review of control of cell fate and proliferation in colon cancer PMID: 25193262 miR-603 regulates glioma development via Wnt-beta-cateninn signaling pathway and its WIF1 and CTNNBIP1 targets. PMID: 25681036 These results indicate that beta-catenin translation is initiated via the IRES and this is regulated by PTX, suggesting that regulation of the IRES-dependent translation of beta-catenin may be involved in the cancer cell response to PTX treatment. PMID: 25849888 ICAT may serve as a tumor-suppressor in human glioma. PMID: 25434796 Suppressing TrpC5 expression decreased nuclear beta-catenin accumulation, reduced the induction of ABCB1, and reversed 5-fluorouracil neoplastic resistance. PMID: 25404731 Our results indicate that HDAC1 plays an important role in chondrogenesis and may represent a therapeutic target for modulation of cartilage development. PMID: 25445594 ASPP2 prevents beta-catenin from transactivating ZEB1 directly by forming an ASPP2-beta-catenin-E-cadherin ternary complex PMID: 25344754 It involved in the control of cell growth, differentiation, and apoptosis. PMID: 24390805 PTHrP was found to inhibit DKK1 expression through c-Jun-mediated inhibition of beta-catenin activity on the DKK1 promoter. PMID: 23752183 beta-catenin inhibitor ICAT modulates the invasive motility of melanoma cells. PMID: 24514042 Neither cyclin D1 nor beta-catenin was expressed in a statistically significant manner, concluding that the development of MCCs is independent of beta-catenin and cyclin D1 expression and these proteins are not suitable as prognostic markers. PMID: 23928935 beta-catenin is delocalized and survivin is over-expressed in recurrent sporadic and nevoid basal cell carcinoma syndrome-associated keratocystic odontogenic tumor PMID: 23572266 Thus, our results indicated abnormal expression of CD44V6, CDH11, and beta-catenin in osteosarcomas and osteochondromas, which may provide important indicators for further research. PMID: 23971040 The data show that Snail1 and beta-catenin, besides association with loss of hormone dependence, protect cancer cells from hypoxia and may serve as an important target in the treatment of breast cancer. PMID: 23973669 This study demonistrated that mean protein and mRNA levels of beta-catenin were significantly decreased in both the PFC and hippocampus of teenage suicide group compared to controls. PMID: 23110823 beta-catenin accumulation in the nucleus regulates expression of Nanog protein and has a role in progression of non-small cell lung cancer PMID: 23648139 These findings suggest that Sphingosine-1-phosphate activates signaling leading to nuclear translocation of beta-catenin in osteoblast-like cells and upregulation of osteoptotegerin and osteoblast differentiation markers PMID: 23612487 beta-catenin was significantly associated with nodal stage, TNM stage, and E-cadherin expression. beta-catenin could be early marker for identification of occult metastases in oral SCC. PMID: 22525043 In this review, we will discuss the points of intersection between the Wnt/beta-catenin and Hippo/YAP pathways and how these interactions contribute to homeostasis, organ repair, and tumorigenesis. PMID: 23027379 ICAT is a novel Ptf1a interactor that regulates pancreatic acinar differentiation and displays altered expression. PMID: 23339455 our data clearly identify protein stabilizing mutations of the beta-catenin gene as a common feature of nested stromal epithelial tumors of the liver, similarly as in hepatoblastomas. PMID: 22749188 Microatellite-stable cases exhibited reduced membranous beta catenin and increased cytoplasmic and nuclear beta catenin compared with microsatellite-instable cases. PMID: 21791486 Airway basal cell beta-catenin determines cell fate and its mis-expression is associated with the development of human lung cancer. PMID: 22081448 Cholangiocarcinoma cell lines express E-cad and beta-cat but with different localizat- ion patterns. PMID: 22075503 Inhibitor of beta-catenin and T-cell factor (ICAT), a beta-catenin binding protein that inhibits the canonical Wnt/beta-catenin signaling pathway, in AR signaling, was investigated. PMID: 21885566 Reduced/aberrant beta-catenin expression was seen in benign and malignant salivary gland tumors. PMID: 20034989 ICAT is a direct transcriptional target of E2F1, and activation of ICAT by E2F1 is required for E2F1 to inhibit beta-catenin activity. PMID: 21532622 overexpression of beta-catenin may be an important contributing factor to glioma progression. PMID: 20300972 High beta-catenin is associated with glioma progression. PMID: 21636708 LMP1 may be involved in nasopharyngeal carcinogenesis via beta-catenin signaling pathway PMID: 21336584 A coactivator role of CARM1 in the dysregulation of beta-catenin activity in colorectal cancer cell growth and gene expression PMID: 21478268 When both E-cadherin and beta -catenin expressions were reduced, there was a significant unfavourable prognosis. PMID: 21574102 The protein expression levels of Wnt1, beta-catenin and Cyclin D1 were all positively correlated with the Karnofsky performance scale (KPS) score and World Health Organization (WHO) grades of patients with gliomas. PMID: 20809334 beta-catenin is expressed in actinic chelitis and squamous cell carcinoma of the lip PMID: 20970365 Data show that elevated beta-catenin expression level may be an adverse indicator for the prognosis of ESCC patients at stage T2-3N0M0, especially for those with T3 lesions or stage IIB diseases. PMID: 21049553 cytoplasmic accumulation of beta-catenin can induce Tcf/Lef-mediated transcriptional activity, up-regulate MMP-7, and induce epithelial and mesenchymal transition (EMT). PMID: 20878057 our results support the concept that STAT3 upregulates the protein expression and transcriptional activity of beta-catenin in breast cancer. PMID: 20830236

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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