Recombinant Human BCL10 Protein (His Tag)

Name: Recombinant Human BCL10 Protein (His Tag)
Brand: Beta LifeScience
SKU: BL-100PC
Availability: InStock

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Product Overview

Tag	His
Host Species	Human
Synonym	c-E10; CARMEN; CIPER; CLAP; mE10 and B-cell CLL/lymphoma 10.
Background	Bcl-10 is an intracellular signaling protein identified from the t(1;14)(p22;q32) breakpoint in MALT lymphomas. The human Bcl10 gene encodes a protein of 233 amino acids containing an N-terminal caspase recruitment domain (CARD) that mediates self-oligomerization and the C-terminal region of Bcl-10 (1-3). Bcl 10 is expressed ubiquitously with high expression levels in lymphoid tissues and in the developing central nerve system (4-6). Mutations have been found in cases of follicular lymphoma and diffuse large B cell lymphoma (7). Bcl-10 is a positive regulator of antigen-receptor induced NF-kB activation and Bcl-10-initiated activation of NF-kB can be inhibited by cotransfection of dominant-negative mutants of TRAF2, NIK, IKKa or IkBa (3-5, 8).
Description	Recombinant Human BCL10 Protein was expressed in Baculovirus-insect cell. This protein is fused with a His tag and purified using our unique purification methods.
Source	Baculovirus-insect cell
AA Sequence	MEPTAPSLTE EDLTEVKKDA LENLRVYLCE KIIAERHFDH LRAKKILSRE DTEEISCRTSSRKRAGKLLD YLQENPKGLD TLVESIRREK TQNFLIQKIT DEVLKLRNIK LEHLKGLKCSSCEPFPDGAT NNLSRSNSDE SNFSEKLRAS TVMYHPEGES STTPFFSTNS SLNLPVLEVGRTENTIFSST TLPRPGDPGA PPLPPDLQLE EEGTCANSSE MFLPLRSRTV SRQ
Purity	>90% purity by SDS-PAGE
Endotoxin	<0.1 ng/µg (1 EU/µg) as determined by LAL test.
Bioactivity	1 unit equals 1 nanogram of purified protein.
Formulation	The human BCL10 protein is supplied in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
Usage	For Research Use Only
Storage	Stored the protein at -70°C before use. Avoid repeated freeze thaw cycles.

Target Details

Target Function	Plays a key role in both adaptive and innate immune signaling by bridging CARD domain-containing proteins to immune activation. Acts by channeling adaptive and innate immune signaling downstream of CARD domain-containing proteins CARD9, CARD11 and CARD14 to activate NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Recruited by activated CARD domain-containing proteins: homooligomerized CARD domain-containing proteins form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10, subsequent recruitment of MALT1 and formation of a CBM complex. This leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Activated by CARD9 downstream of C-type lectin receptors; CARD9-mediated signals are essential for antifungal immunity. Activated by CARD11 downstream of T-cell receptor (TCR) and B-cell receptor (BCR). Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK.
Subcellular Location	Cytoplasm, perinuclear region. Membrane raft.
Database References	HGNC: 989 OMIM: 137245 KEGG: hsa:8915 STRING: 9606.ENSP00000271015 UniGene: Hs.193516
Associated Diseases	Immunodeficiency 37 (IMD37); Lymphoma, mucosa-associated lymphoid type (MALTOMA)
Tissue Specificity	Ubiquitous.

Gene Functions References

Results show that GSK3beta modulates serine phosphorylation of BCL10. PMID: 29358699
BCL10 forms CARMA1-BCL10-MALT1-TRAF6 signalosome and BCL10 polymerizes in a unidirectional manner. PMID: 29382759
The results suggest that the involvement of BCL10 in DNA damage-induced NF-kappaB is through the recruitment of TRAF6. PMID: 28717989
results define molecular determinants that control the production of Lin(Ub)n-Bcl10, an important signaling intermediate in TCR and oncogenic CARD11 signaling. PMID: 27777308
Psoriasis mutations disrupt CARD14 autoinhibition promoting BCL10-MALT1-dependent NF-kappaB activation PMID: 27071417
BCL10 promoted DNA double-strand breaks repair, enhancing cell survival after DNA damage. PMID: 26771713
Data show that caspase recruitment domain-containing protein 11/B-cell CLL/lymphoma 10/mucosa-associated lymphoid tissue lymphoma translocation gene 1 signaling drives lymphoproliferation through NF-kappa B and c-Jun N-terminal kinase activation. PMID: 26668357
BCL10 is not essential for actin polymerization after fibroblast FcgammaR stimulation. PMID: 26774590
characterization of zebrafish (Danio rerio) Bcl10 PMID: 25849213
we identified BCL10 as a bona fide target of BCR-induced linear ubiquitylation and demonstrated an important role of the linear ubiquitin ligase HOIP in BCR-induced phosphorylation PMID: 26038114
The results of this study indicate that inherited BCL10 deficiency should be considered in patients with combined immunodeficiency with B cell, T cell, and fibroblast defects. PMID: 25365219
B-cell lymphoma/leukemia 10 promotes oral cancer progression through STAT1/ATF4/S100P signaling pathway. PMID: 24681956
BCL10 induces cleavage of MALT1 at R149 in 293T cells. PMID: 25105596
Overexpression of CARMA-BCL10-MALT in T-ALL may contribute to the constitutive cleavage and inactivation of A20, which enhances NF-kappaB signaling and may be related to T-ALL pathogenesis. PMID: 25384343
BCL10 delivers UBC13 to RNF8/RNF168 to regulate ubiquitination-mediated double-strand break signaling and repair. PMID: 24732096
elevated in cholesteatoma PMID: 24702227
A novel susceptibility locus on 1p22 was discovered, which implicates BCL10 as a new susceptibility gene for leprosy. PMID: 23784377
Combining crystallography, nuclear magnetic resonance, and electron microscopy, we reveal the structure of the Bcl10 CARD filament and the mode of interaction between CARMA1 and Bcl10 PMID: 24074955
BCL10 expression is a useful marker for acinar cell differentiation, particularly in the diagnosis of endoscopic ultrasound-guided fine-needle aspiration specimens of Acinar cell carcinomas of the pancreas PMID: 23530562
Bcl10 links saturated fat overnutrition with hepatocellular NF-kB activation and insulin resistance. PMID: 22708078
BCL10 was commonly down-regulated in peripheral T cell lymphomas, suggest the T-cell receptor signaling cascade for future characterization. PMID: 22818167
identify Bcl10 as an early coordinator of NF-kappaB-mediated immune response with endosomal trafficking and signaling to F-actin remodeling PMID: 23153494
Results indicate that NF-kappaB binding to the BCL10 promoter can lead to prolonged activation of the carrageenan-induced inflammatory cascade by a transcriptional mechanism involving an NF-kappaB-BCL10 loop. PMID: 22579587
A proapoptotic role for protein kinase C zeta in the binding and phosphorylating Bcl10 at the nuclear envelope. PMID: 22812606
BCL10 plays an important role in controlling the growth of cervical cancer cells through NF-kappaB dependent cyclin D1 regulation. PMID: 22564715
FOXO3a promotes cell survival via BCL10/NF-kappaB in serum starvation PMID: 22474286
identify MIB2 as a novel component of the activated BCL10 signaling complex and a missing link in the BCL10-dependent NF-kappaB signaling pathway. PMID: 21896478
findings indicate that BCL10 phosphorylations act upstream of phosphorylations of NIK, TAK1, and IkappaBalpha and differentially affect the canonical and noncanonical pathways of NF-kappaB activation PMID: 21700900
Findings demonstrate that CaN functions as a critical signaling molecule during Th cell activation, regulating Bcl-10 phosphorylation and NF-kappaB activation. PMID: 21674474
study shows CaMKII is recruited to the immunological synapse where it interacts with and phosphorylates Bcl10; propose a mechanism whereby Ca(2+) signals can be integrated at the immunological synapse through CaMKII-dependent phosphorylation of Bcl10 PMID: 21513986
Data show that CARMA3 and Bcl10 contributed to several characteristics of EGFR-associated malignancy, including proliferation, survival, migration, and invasion. PMID: 21406399
Lipopolysaccharide induces activation of both canonical and non-canonical pathways of NF-kappaB and the non-canonical pathway requires phosphorylations of BCL10 (serine 138) and NIK. PMID: 20466000
A novel mutation of Bc1-10 gene in ocular adnexal MALT lymphoma was detected in Chinese patients. PMID: 18307945
BCL10 nuclear expression is common in ocular adnexal mucosa-associated lymphoid tissue lymphomas. PMID: 19035248
activation of NF-kappaB by CXCR4 occurs through Carma3/Bcl10/Malt1 (CBM) complex in OSCC. loss of components of CBM complex in HNSCC can inhibit SDF-1 alpha induced phosphorylation and degradation of IkappaBalpha. PMID: 20695076
Interaction of calmodulin with Bcl10 modulates NF-kappaB activation. PMID: 20439115
A specific single nucleotide polymorphism in the BCL10 gene may be responsible for the tumorigenesis of intracranial germinomas in Japanese individuals. PMID: 19690445
Results suggest that the NFkappaB regulator BCL10 is an IL-2-independent STAT5 target gene. PMID: 19709433
These findings indicate an upstream signaling role for BCL10, in addition to its effects on IKKgamma, the regulatory component of the IKK signalosome, and a requirement for BCL10 in both canonical and noncanonical pathways of NF-kappaB activation. PMID: 19897484
vimplicated in apoptosis, and it has been suggested that mutated forms gain oncogenic activity. The occurrence of genomic BCL10 mutations in gastric MALT-type lymphomas was investigated. PMID: 11830492
Mutations, relatively common in lymphomas, are extremely rare in malignant cartilaginous tumors. PMID: 11836626
REVIEW: Genetic alterations involving BCL10 underlying the pathogenesis of MALT lymphoma PMID: 11960389
There is a lack of BCL10 mRNA mutation in lymphold malignancies. PMID: 12017308
association of mutations with aberrant BCL10 localization in the nucleus in nasal NK/T-cell lymphomas PMID: 14523480
Bcl10 is post-translationally modified by Rip2 and has a role in T-cell signaling PMID: 14638696
Nuclear expression of BCL10 is unlikely to correlate with the API2-MALT1 fusion gene in ocular adnexal MALT lymphoma. PMID: 14674990
Together, these findings suggest that Bcl10 nuclear expression may modulate gene expression and Bcl10 is a potential transcriptional activator apart from its traditional roles that have been found. PMID: 15207693
BinCard inhibits BCL10-mediated activation of NF-kappa B. PMID: 15637807
The ability of Bcl10 expression to prevent B-cell antigen receptor-induced growth arrest and apoptosis of WEHI-231 cells was dependent on NF-kappaB activation. PMID: 15878976
nucleocytoplasmic shuttling of MALT1 and BCL10 complex may indicate that these molecules are involved not only in the nuclear factor kappaB (NF-kappaB) pathway but also in other biologic functions in lymphocytes PMID: 16123224

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

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Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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