Recombinant Human B-Cell Scaffold Protein With Ankyrin Repeats (BANK1) Protein (His)

Beta LifeScience SKU/CAT #: BLC-10372P
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SDS-PAGE.

Recombinant Human B-Cell Scaffold Protein With Ankyrin Repeats (BANK1) Protein (His)

Beta LifeScience SKU/CAT #: BLC-10372P
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Product Overview

Description Recombinant Human B-Cell Scaffold Protein With Ankyrin Repeats (BANK1) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb Q8NDB2
Target Symbol BANK1
Synonyms BANK1B-cell scaffold protein with ankyrin repeats
Species Homo sapiens (Human)
Expression System Yeast
Tag N-6His
Target Protein Sequence MLPAAPGKGLGSPDPAPCGPAPPGNTKDIIMIYEEDAEEWALYLTEVFLHVVKREAILLYRLENFSFRHLELLNLTSYKCKLLILSNSLLRDLTPKKCQFLEKILHSPKSVVTLLCGVKSSDQLYELLNISQSRWEISTEQEPEDYISVIQSIIFKDSEDYFEVNIPTDLRAKHSGEISERKEIEELSEASRNTIPLAVVLPTEIPCENPGEIFIILRDEVIGDTVEVEFTSSNKRIRTRPALWNKKVWCMKALEFPAGSVHVNVYCDGIVKATTKIKYYPTAKAKECLFRMADSGESLCQNSIEELDGVLTSIFKHEIPYYEFQSLQTEICSQNKYTHFKELPTLLHCAAKFGLKNLAIHLLQCSGATWASKMKNMEGSDPAHIAERHGHKELKKIFEDFSIQEIDINNEQENDYEEDIASFSTYIPSTQNPAFHHESRKTYGQSADGAEANEMEGEGKQNGSGMETKHSPLEVGSESSEDQYDDLYVFIPGADPENNSQEPLMSSRPPLPPPRPVANAFQLERPHFTLPGTMVEGQMERSQNWGHPGVRQETGDEPKGEKEKKEEEKEQEEEEDPYTFAEIDDSEYDMILANLSIKKKTGSRSFIINRPPAPTPRPTSIPPKEETTPYIAQVFQQKTARRQSDDDKFCGLPKKQDRARIESPAFSTLRGCLTDGQEELILLQEKVKNGKMSMDEALEKFKHWQMGKSGLEMIQQEKLRQLRDCIIGKRPEEENVYNKLTIVHHPGGKETAHNENKFYNVHFSNKLPARPQVEKEFGFCCKKDH
Expression Range 1-785aa
Protein Length Full Length
Mol. Weight 91.3kDa
Research Area Immunology
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Involved in B-cell receptor (BCR)-induced Ca(2+) mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2.
Database References

HGNC: 18233

OMIM: 152700

KEGG: hsa:55024

STRING: 9606.ENSP00000320509

UniGene: PMID: 30096841

  • our results indicated that the BLK rs13277113 polymorphism was involved in the genetic background of RA in Chinese population and the association of BANK1 rs3733197 polymorphism with RA was dependent on the genotype of BLK rs13277113 polymorphism, highlighting B-cell response implicated in the pathogenesis of RA. PMID: 28925718
  • This meta-analysis demonstrates that BANK1 rs10516487, rs3733197, and rs17266594 polymorphisms are associated with susceptibility to autoimmune diseases. PMID: 28466820
  • Confirm the association of rs548234/ATG5, rs2736340/BLK and rs10516487/BANK1 with systemic lupus erythematosus in Chinese Han and reinforced our hypothesis of their epistasis effect in regulating B-cell signaling in SLE. PMID: 26420661
  • The results of this study suggesting a mechanism for the involvement of BANK1 in the BOLD response to working memory PMID: 27671502
  • Single-nucleotide polymorphism in BANK1 gene is associated with sclerotic graft-versus-host disease. PMID: 27313329
  • The SLE susceptibility variants in the BANK1 gene may contribute to lupus by altering B cell signaling, increasing FOXO1 levels, and enhancing memory B cell development PMID: 27816669
  • The systemic lupus erythematosus variant Ala71Thr of BLK severely decreases protein abundance and binding to BANK1 through impairment of the SH3 domain function. PMID: 26821283
  • Data show that single-nucleotide polymorphism rs3733197 located in the coding region of BANK1 (B-cell scaffold protein with ankyrin repeats 1) was significantly associated with serum LDL cholesterols. PMID: 25608828
  • Authors identified a novel balanced reciprocal translocation, t(4;14)(q24;q32.) Sequence analysis identified the partner gene as BANK1. PMID: 24879116
  • Our study confirms evidence for epistasis between BLK and BANK1 in systemic lupus erythematosis from a Chinese population for the first time. PMID: 24085759
  • Our findings suggest the existence of association between BANK1 gene and autoimmune thyroid diseases PMID: 24127308
  • BANK1 and IL15 as new T1D genetic susceptibility factors. PMID: 24342660
  • BANK1 and BLK have roles in B-cell signaling through phospholipase C gamma 2 PMID: 23555801
  • SNPs of the FAM167A-BLK region, but not the BANK1 SNPs, were associated with the development of primary Sjogren's syndrome in Han Chinese. PMID: 23899688
  • This study confirms BANK1 as an RA susceptibility gene and for the first time provides evidence for epistasis between BANK1 and BLK in RA. PMID: 23646104
  • This study did not reveal a major role for BANK1 in humoral autoimmunity in chronic arthritis. PMID: 23065315
  • short Delta2 isoform of BANK1 displays a homogeneous cytoplasmic distribution PMID: 21900951
  • Polymorphism in BANK1 is associated with susceptibility to psoriasis in Southern Han Chinese. PMID: 21989138
  • allelic variation in BANK1 does not play a major role in determining multifocal motor neuropathy susceptibility. PMID: 22003931
  • This study implies that rs10516487 and rs17266594 polymorphisms might contribute to individual susceptibility to SLE PMID: 21078628
  • This study shows a genetic interaction between BANK1 and BLK, and demonstrates that these molecules interact physically. PMID: 21978998
  • This meta-analysis demonstrates a significant association between BANK1 and TNFAIP3 gene polymorphism and systemic lupus erythematosus in multiple ethnic populations. PMID: 21208380
  • Results do not support a major implication of the BANK1 locus in susceptibility to giant cell arteritis. PMID: 20472928
  • results suggest that the BANK1 gene confers susceptibility to systemic sclerosis in general, and specifically to the diffuse cutanenous systemic sclerosis and anti-topoisomerase I antibody subsets. PMID: 19815934
  • Functional variants in the B-cell gene BANK1 are assoicated with systemic lupus erythematosus. PMID: 18204447
  • These results suggest that BANK1 SNPs and haplotypes may contribute to rheumatoid arthritis susceptibility with a low risk. PMID: 19180476
  • Study independently replicates and confirms the strong association of BANK1 variants rs17266594 and rs10516487 with the risk of systemic lupus erythematosus in the European-derived population. PMID: 19339986
  • Study confirmed the associations of BANK1 (rs3733197)and TNFSF4 (rs844648) with systemic lupus erythematosus in Hong Kong Chinese. PMID: 19357697
  • STAT4 and BLK displayed a strong genetic association with primary antiphospholipid syndrome, while a weak association with IRF5 and no association with BANK1 were observed. PMID: 19644876
  • BANK1 is a new systemic sclerosis genetic susceptibility factor. BANK1, IRF5, and STAT4 act with additive effects. PMID: 19877059
  • FAQs

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

    To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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