Recombinant Human Atrial Natriuretic Peptide Receptor 3 (NPR3) Protein (His)

Name: Recombinant Human Atrial Natriuretic Peptide Receptor 3 (NPR3) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04946P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Human Atrial Natriuretic Peptide Receptor 3 (NPR3) Protein (His) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P17342
Target Symbol	NPR3
Synonyms	NPR3; ANPRC; C5orf23; NPRC; Atrial natriuretic peptide receptor 3; Atrial natriuretic peptide clearance receptor; Atrial natriuretic peptide receptor type C; ANP-C; ANPR-C; NPR-C
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-10His
Target Protein Sequence	GGVGGGGGGAGIGGGRQEREALPPQKIEVLVLLPQDDSYLFSLTRVRPAIEYALRSVEGNGTGRRLLPPGTRFQVAYEDSDCGNRALFSLVDRVAAARGAKPDLILGPVCEYAAAPVARLASHWDLPMLSAGALAAGFQHKDSEYSHLTRVAPAYAKMGEMMLALFRHHHWSRAALVYSDDKLERNCYFTLEGVHEVFQEEGLHTSIYSFDETKDLDLEDIVRNIQASERVVIMCASSDTIRSIMLVAHRHGMTSGDYAFFNIELFNSSSYGDGSWKRGDKHDFEAKQAYSSLQTVTLLRTVKPEFEKFSMEVKSSVEKQGLNMEDYVNMFVEGFHDAILLYVLALHEVLRAGYSKKDGGKIIQQTWNRTFEGIAGQVSIDANGDRYGDFSVIAMTDVEAGTQEVIGDYFGKEGRFEMRPNVKYPWGPLKLRIDENRIVEHTNSSPCKSSGGLEE
Expression Range	27-481aa
Protein Length	Partial
Mol. Weight	54.0 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	ANF receptor family
Database References	HGNC: 7945 OMIM: 108962 KEGG: hsa:4883 STRING: 9606.ENSP00000265074 UniGene: PMID: 28659173 This study suggested that NPR3 rs2270915 polymorphism was associated with decreased systolic blood pressure level marginally in essential hypertension patients in a Chinese Han population; the polymorphism may function through decreasing NPR3 mRNA expression and ANP level. PMID: 28497617 NPR-C gene single nucleotide polymorphisms significantly contribute to CAD susceptibility in the Chinese Han population PMID: 27191271 The NPRC genetic variant,Rs1847018, is a genetic marker of essential hypertension. PMID: 26782497 This study focused on the natriuretic peptide receptor C gene (NPR3). The correlation analysis between NPR3 and hypertension was replicated in 450 Chinese Dai and 484 Chinese Mongolian individuals. PMID: 26345810 Angiotensin II downregulates vascular smooth muscle cell NPR-C gene expression by destabilizing its mRNA. PMID: 25711724 Results from this study point to a role for miR-100 in the regulation of NPR3 expression, and suggest a possible therapeutic target for modulation of NP bioactivity in heart disease. PMID: 25736855 Data show that natriuretic peptide receptor 3 (NPR3) single nucleotide polymorphism (SNP) is independently associated with diastolic dysfunction and does not appear to be related to alterations in circulating levels of natriuretic peptides. PMID: 24465655 The significant change in NPR3 protein was observed for the Arg146 variant allozyme, with 20% of wild-type protein, primarily because of autophagy-dependent degradation. PMID: 23493048 polymorphisms or haplotype in NPR3 gene may influence the risk of ischemic stroke or hypertension independently in Chinese population. PMID: 22559095 a NPR3 promoter gene variant could have a role on cerebrovascular disease susceptibility PMID: 22995222 Report the presence of CNP and its receptors, NPR2/3 in atherosclerotic plaques of human carotid artery, with increased expression of NPR3 in histologically unstable plaques. PMID: 22421372 Data found a consistent and significant association between the rs2270915 polymorphism of the NPR3 gene and SBP in diabetic patients. This genetic variation may affect pressure response to changes in dietary sodium. PMID: 21464461 integrative genomics approach to a large cohort of medulloblastomas has identified four disparate subgroups (NPR3)with distinct demographics, clinical presentation, transcriptional profiles, genetic abnormalities, and clinical outcome. PMID: 20823417 The mRNA levels of natriuretic peptide receptor-C appear to be increased in epicardial adipose tissue independent of their plasma levels in coronary artery disease. PMID: 20691218 The novel Npr1 gene 3C variant and the Npr3 gene C(-55) allele are associated with hypertensive family history. PMID: 12872042 Atrial natriuretic paptide, purified from medium bathing cells expressing NPR-C, a receptor known to internalize natriuretic peptides, was degraded. PMID: 15459247 Novel six-nucleotide repeat polymorphism located 4 base pairs upstream of the major transcriptional initiation site. PMID: 15785005 model of hormone recognition and allosteric receptor activation revealed from structural studies of NPR-C [review] PMID: 15911071 discussion of NPR-C receptor coupling to different signaling pathways and their regulation [review] PMID: 15911072 A structural comparison of complexes of NPC-C with each NP hormones (ANP, BNP, and CNP)reveals that NPR-C uses a conformationally inflexible surface to bind three different, highly flexible, NP ligands. PMID: 16870210 The NPRC polymorphism is not an independent determinant of NP concentration in HF. PMID: 17890443 the NPR-C receptor is expressed in normal and neoplastic human alpha cells. PMID: 19352691

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.