Human ATP4A (Potassium-Transporting Atpase Alpha Chain 1) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-08844P
SDS-PAGE analysis of Human ATP4A (Potassium-Transporting Atpase Alpha Chain 1) - Recombinant Protein, CAT# BLT-08844P showing >90% purity under 15% SDS-PAGE (Reduced)
Human ATP4A (Potassium-Transporting Atpase Alpha Chain 1) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-08844P
Regular price
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Quantity Pricing
| Pack Size | Price (USD) |
|---|---|
| 500 µg | $1,375 |
| 1 mg | $2,245 |
For direct online orders, quantity pricing will be displayed in cart when you add 5x100ug or 10x100ug
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Product Overview
| Product Name | Recombinant Human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) Protein |
| Product Overview | This recombinant human ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) protein includes amino acids 798-896 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id | P20648 |
| Recommended Name | Potassium-transporting ATPase alpha chain 1 |
| Gene Name | ATP4A |
| Synonyms | Gastric H,K-ATPase Alpha Subunit; H(+)-K(+)-ATPase Alpha Subunit; Proton Pump; Potassium-transporting ATPase alpha chain 1 |
| Species | Human |
| Predicted Molecular Mass | 17kDa |
| Expression System | E.coli |
| Expression Range | 798-896 |
| Tag | N-6His |
| Purity | >90% |
| Formulation | Lyophilized |
| Buffer | PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications | Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area | Others |
| Target Function | The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state. |
| Subcellular Location | Apical cell membrane; Multi-pass membrane protein. |
| Protein Family | Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIC subfamily |
| Tissue Specificity | Expressed in gastric parietal cells (at protein level). |
![SDS-PAGE analysis of Human NDUFV1 (Nadh Dehydrogenase [Ubiquinone] Flavoprotein 1, Mitochondrial) - Recombinant Protein, CAT# BLT-08321P, showing >90% purity under 15% SDS-PAGE (Reduced)](http://www.betalifesci.com/cdn/shop/files/1ihmbkjr21vdmc6j_{width}x.jpg?v=1753363793)
