Recombinant Human Asporin (ASPN) Protein (His-SUMO)

Name: Recombinant Human Asporin (ASPN) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03812P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Asporin (ASPN) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9BXN1
Target Symbol	ASPN
Synonyms	ASPN; ASPN protein; ASPN_HUMAN; Asporin (LRR class 1); Asporin; Asporin proteoglycan; FLJ20129; LRR class 1; Periodontal ligament associated protein 1; Periodontal ligament-associated protein 1; PLAP 1; PLAP-1; PLAP1; SLRR 1C; SLRR1C; Small leucine rich protein 1C
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	DMEDTDDDDDDDDDDDDDDEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIARVGVNDFCPTVPKMKKSLYSAISLFNNPVKYWEMQPATFRCVLSRMSVQLGNFGM
Expression Range	33-380aa
Protein Length	Full Length of Mature Protein
Mol. Weight	55.7kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Protein Families	Small leucine-rich proteoglycan (SLRP) family, SLRP class I subfamily
Database References	HGNC: 14872 OMIM: 603932 KEGG: hsa:54829 STRING: 9606.ENSP00000364694 UniGene: PMID: 30407347 Suggest that the D14 allele of the ASPN polymorphism could exert an influence on primary osteoarthritis of the knee etiology in a Mexican Mestizo population. PMID: 26620055 ASPN D-repeat polymorphism is not associated with an increased Knee Osteoarthritis risk. PMID: 28889984 Meta-analysis result based on previously published studies demonstrated that the ASPN D13 allele was a protective factor for OA of knee, hip, and hand. For D14 and D15 allele, meta-analysis did not demonstrate statistically significant association. PMID: 29561445 higher expression of asporin was noticed in CRC tissues and it was correlated with later clinical stage of the patients. Asporin promoted the migration and invasion of the tumor cells partially through an EGFR/Src/cortactin- signaling pathway. PMID: 27705916 ASPN D15 is associated with increased risk of symmetrical hand osteoarthritis particularly in individuals with low variation in work tasks. PMID: 29233086 we found that asporin can be downregulated by bone morphogenetic protein 4 in Hs578T cells and its upregulation may be facilited by serum-free cultivation or by three dimensional growth PMID: 27409832 Asporin promotes epithelial mesenchymal transformation, invasion, and migration of pancreatic cancer cellss by activating CD44-AKT/ERK-NF-kappaB pathway in paracrine and autocrine manner. PMID: 28400334 High ASPN expression in stroma is associated with prostate cancer progression. PMID: 28152543 To test the associations of ASPN variations with risk of subsequent oncologic outcomes. PMID: 26446945 Collectively, our findings indicate that ASPN is upregulated and plays an oncogenic role in gastric cancer progression and metastasis by influencing the EGFR signaling pathway. PMID: 25673058 Our results showed that ASPN rs13301537 T to C change and variant C genotype may contribute to knee OA risk in a Chinese Han population. PMID: 25030405 this is the first case-control study in Mexican women that suggests that menopause and the D-repeat polymorphism in the ASPN gene are associated with knee OA PMID: 26016288 Osteomodulin, osteoglycin, and asporin appear to be distinctly regulated in osteoarthritis labrum compared to OA cartilage. PMID: 25371314 Asporin may represent a new therapeutic target molecule for the development of drugs aimed at manipulating the cancer microenvironment. PMID: 24441039 This meta-analysis shows that the ASPN D14, D13, and D15 alleles are not associated with the development of osteoarthritis in Europeans and Asians. [Meta-Analysis] PMID: 24306268 A meta-analysis suggest that the D-repeat of asporin gene (ASPN) may not be a major susceptibility locus in the Caucasian and Asian populations with knee osteoarthritis. PMID: 23942062 polymorphisms within the ASPN gene could influence knee osteoarthritis susceptibility PMID: 23733110 D14-PLAP-1 suppressed BMP-2 signal transduction more efficiently than D13-PLAP-1; stronger affinity of D14-PLAP-1 protein to BMP-2 compared with D13-PLAP-1 protein. D repeat polymorphism of PLAP-1/asporin has influence on functions of PDL cells. PMID: 24453179 Our data suggest that the D15 asporin allele could be considered a knee osteoarthritis risk allele significant only for women in the Iranian population. PMID: 24078942 The asporin-encoding gene is a promising candidate as a susceptibility gene for osteoarthritis and degenerative disc disease. [Review] PMID: 24003854 Asporin is associated with hand osteoarthritis progression. PMID: 23357225 mir-101 and mir-21 target PLAP-1 to regulate its expression during osteogenic differentiation of PDLCs. PMID: 22367347 Our results show an obvious association between the D repeat polymorphism of ASPN and Developmental dysplasia of the hip PMID: 21329514 Data indicate that the expression of ASPN gene is finely regulated in cartilage and suggest a major role of Sp1. PMID: 21528154 ASPN plays positive roles in the mineralization of dental pulp stem cells and predentin to dentin. PMID: 21413025 The ratio of Asporin to TGF-beta1 (transforming growth factor-beta1) mRNA in patients with severe cartilage damage was higher than that in osteoartritis patients with mild cartilage damage PMID: 19997821 Association of an asporin repeat polymorphism with ankylosing spondylitis in Han Chinese population: a case-control study, is reported. PMID: 20144272 these findings provide another functional link between extracellular matrix proteins, TGF-beta activity and disease, suggesting new therapeutic strategies for osteoarthritis. PMID: 15640800 No significant differences were observed in any of the multiple comparisons performed in osteoarthritis and controls PMID: 16542493 expression may be associated with the process of cytodifferentiation of periodontal ligament cells PMID: 16632759 Association of the D14 allele with knee osteoarthritis susceptibility in Han Chinese. PMID: 17024313 the association of the ASPN D14 allele and knee OA has global relevance [meta-analysis] PMID: 17517696 This study further highlighted the significance of asporin in osteoarthritis. PMID: 17603749 Characterization of the human ASPN promoter region revealed a region from -126 to -82 that is sufficient for full promoter activity; however, TGF-beta1 failed to increase activity through the ASPN promoter. PMID: 17804408 describe mechanisms for asporin function and regulation in human articular cartilage; asporin blocks chondrogenesis and inhibits TGF-beta1-induced expression of matrix genes and the resulting chondrocyte phenotypes. PMID: 17827158 The frequency of the aspartic acid repeat polymorphism was examined. These results suggest that asporin may play a role in OA susceptibility of the knee in the Korean female population. PMID: 18178444 ASPN is a lumbar-disc degeneration (LDD) gene in Asians, and common risk factors may be considered for osteoarthritis and LDD. PMID: 18304494 Asporin is up-regulated in disease states. It binds to various growth factors, including TGF-beta and BMP-2, and negatively regulates their activity. By inhibiting binding of TGF-beta1 to its type II receptor, asporin forms a functional feedback loop. PMID: 18336287 These data suggest that polymorphisms within ASPN are not a major influence in susceptibility to hand or knee OA in US Caucasians. PMID: 18434216 In the discs of Caucasian subjects there was greatest expression of asporin in the more degenerate human discs in vivo. PMID: 19327154 Asporin has a role in osteoblast-driven collagen biomineralization activity. PMID: 19589127

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.