Recombinant Human ANG2 Protein (C-6His)

Name: Recombinant Human ANG2 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-2212NP
Availability: InStock

BL-2212NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Human Angiopoietin-2 is produced by our Mammalian expression system and the target gene encoding Lys275-Phe496 is expressed with a 6His tag at the C-terminus.
Accession	O15123
Synonym	AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
Gene Background	Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that plays a complex role in angiogenesis and inflammation. Both Ang-2 and the related Angiopoietin-1 (Ang-1) are ligands for the receptor tyrosine kinase Tie-2. While Ang-1 is a potent Tie 2 agonist, Ang-2 may act as either a Tie-2 antagonist or agonist, depending upon its state of multimerization. The higher the order of oligomer, the more effective Ang-2 becomes as a Tie-2 agonist. The short isoform appears to block the binding of either Ang-1 or full-length Ang-2 to Tie-2. Ang-2 functions as a pro-angiogenic factor, although it can also induce EC death and vessel regression. Upon its release from quiescent EC, it regulates vascular remodeling by promoting EC survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.
Molecular Mass	23.6 KDa
Apmol Mass	30-35 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20 mM MOPS, 150 mM NaCl, 0.1% CHAPS, pH 7.5.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Subcellular Location	Secreted.
Database References	HGNC: 485 OMIM: 601922 KEGG: hsa:285 STRING: 9606.ENSP00000314897 UniGene: Hs.583870

Gene Functions References

plasma ANG-2 level, the acute physiology and chronic health evaluation 2 (APACHE2) score and lung injury prediction score are correlated with acute respiratory distress syndrome PMID: 30008611
Study is the first to demonstrate that Ang2 is upregulated not only in the local skin lesions but also in the systemic circulation, and that its serum levels correlate well with disease severity in psoriasis vulgaris patients. PMID: 28497874
regulatory mechanisms of Ang-2 in NSCLC PMID: 30454550
This study demonstrates that Ang-2 levels are significantly upregulated in sepsis-associated coagulopathy (SAC), and this biomarker can be used to risk stratify patients with sepsis into non-overt disseminated intravascular coagulation (DIC) and overt DIC. PMID: 29996658
Flunarizine protected ECs from TNFalpha-induced increase in Angpt-2 transcription and vascular barrier breakdown. PMID: 28276491
Study findings delineate the existence of the beta-estradiol (E2)-ANGPT2 axis in hair follicles and suggest that, by substituting E2, ANGPT2 may provide a novel strategy for the treatment of postmenopausal female pattern hair loss. PMID: 29724581
Results suggest that hCPFs-Exo transports low expressed exosomal miR-106b-5p to endothelial cells and promotes angiogenesis by overexpression of Angpt2. PMID: 29905392
Angpt2 is an independent predictor of adverse clinical outcomes in diabetic patients. Further studies are needed to identify the pathogenic role of Angpt2 in renal deterioration and cardiovascular complications of diabetes mellitus. PMID: 29642068
High ANG2 expression is associated with Acute respiratory distress syndrome. PMID: 30171880
PVT1 was able to bind and degrade miR26b to promote connective tissue growth factor (CTGF) and angiopoietin 2 (ANGPT2) expression. PMID: 29620147
Serum Ang-2 may be a useful tumor marker in predicting liver cancer prognosis. PMID: 29253494
High ANG2 expression is associated with angiogenesis in breast cancer. PMID: 28534941
Angiopoietin-2 acts as a survival factor for chronic lymphocytic leukemia B cells throughout Tie-2 receptor engagement. PMID: 28580615
serum level elevated in pre-eclampsia, not significantly affected by HIV status PMID: 28627965
Plasma levels of Ang2 were associated with markers of malaria severity and levels of var transcripts encoding P. falciparum Erythrocyte Membrane Protein 1 (PfEMP1) containing Cysteine Rich Inter Domain Region alpha1 (CIDRalpha1) domains predicted to bind Endothelial Protein C receptor (EPCR). PMID: 27784899
Hepatitis C virus induces Ang2 expression in hepatocytes. PMID: 28027429
the relationship between lung cancer stage and Ang 2 was documented with this study and the expression rate was found to be lower in adenocarcinomas. By this analysis, we can suggest that angiopoietins may be used as an option for targeted treatment in lung cancer. PMID: 27811442
Endothelial glycocalyx breakdown is mediated by Angpt-2 in a non-redundant manner. PMID: 28453727
The relation between angiopoietin-2 and assessed parameters of vascular structure in type 1 diabetes reflects a state of endothelial injury and highlights the role of disturbed angiogenesis and vascular inflammation in the occurrence of diabetic complications. PMID: 27236773
Results show that MiR-93 targets Ang2 3' UTR and regulates its expression in lung adenocarcinoma. PMID: 28401709
We demonstrate that ANGPT2 signaling activated after estrogen depletion paradoxically triggers ER+ tumor cell awakening from dormancy in their BM niche, partly indirectly via endothelial Tie2 receptor and partly directly via tumor cell surface integrin &1. PMID: 27353038
ANG2 did not affect apoptosis or the cell cycle. In contrast, in the in vivo system, overexpression of ANG2 increased tumor growth. PMID: 27492854
the optimal discrimination cut-off for each cytokine: sVEGFR-1 (2124.5pg/mL), IL-6 (40.2pg/mL), VEGF-A (1060.1pg/mL), Angiopoeintin-2 (913.7pg/mL), uPA (248.1pg/mL), sHER-2/neu (5010pg/mL) and PLGF (93.4pg/mL). For the very first time, a novel cytokine profile associated with cancer metastasis to the paratracheal lymph nodes were reported. PMID: 27599390
Gab1/SHP2/p38MAPK signaling pathway and Ang-2 have an essential role in regulating thrombin-induced monocyte adhesion and vascular leakage PMID: 27241812
This study demonstrated that ANGPT2 higher expression in glioblastoma. PMID: 27633774
These observations provide the first evidence for positive regulation of osteogenesis by ANGPT2 in vitro. PMID: 28214341
in vitro is has also been shown that Angpt-2 can act as a dose-dependent Tie2 agonist/antagonist. PMID: 27038015
Ang-2 and sTie-2 plasma levels are increased in pediatric OSA and obesity, particularly when endothelial dysfunction or insulin resistance is detectable. PMID: 28474375
pathways for two distinctive secretory mechanisms, constitutive and stimulated, of Ang-2 in pulmonary microvascular endothelial cells. PMID: 27585839
High ANG2 expression is associated with angiogenesis and metastasis via IGF1-IGF1R signaling in epithelial ovarian cancer. PMID: 28898232
Our novel noninvasive liver fibrosis model, based on serum angiopoietin-2 levels, outperforms other indices and should help substantially in managing CHC and monitoring long-term follow-up prognosis PMID: 23823085
Tie1 directly interacts with Tie2 to promote ANG-induced vascular responses under noninflammatory conditions, whereas in inflammation, Tie1 cleavage contributes to loss of ANG2 agonist activity and vascular stability PMID: 27548530
This systematic review and meta-analysis suggested that serum Ang-2 levels might be a potential predictor for staging, and were associated with prognosis of lung cancer. PMID: 28906403
Studied angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) within the intervertebral disc (IVD) and elucidated their functions in the regulation of nucleus pulposus (NP) cells. Found the ratio of Ang-2/Ang-1 in tissues from patients increased markedly with increasing age and level of degeneration of the IVD. Results indicate Ang-2 plays a role in suppressing cell adhesion&viability, and promotes the apoptosis of NP cells. PMID: 28394321
Analyses of human gastric cancer tissues showed a strong correlation between DARPP-32 and ANGPT2. The role of DARPP-32-STAT3 axis in regulating ANGPT2 in cancer cells to promote angiogenesis and tumorigenesis. PMID: 25779598
ANGPT2 expression was upregulated in cerebral cavernous malformation lesions. PMID: 27548575
Linkage analysis identified a peak (LOD = 4.29) on chromosome 8p23. Follow-up association analysis identified two haplotypes in angiopoietin-2 (ANGPT2) that significantly contributed to the variation of SaO2 (P = 8 x 10-5) and accounted for a portion of the linkage evidence. PMID: 27798093
Blood ANGPT2 was significantly higher in chronic hepatitis C patients with liver fibrosis compared to those without fibrosis. PMID: 27930387
This study reveals ANGPT2 as a new susceptibility gene for nAMD and PCV, and it may affect disease susceptibility in association with CFH. PMID: 28192798
These results underscore a pivotal role of Kaposi's sarcoma-associated herpesvirus -induced Ang-2 in KS tumor development by promoting both angiogenesis and inflammation. PMID: 27294705
Data suggest that the associations among angiopoietin-2, sFlt-1, coagulation abnormalities and severe course of acute pancreatitis (AP) might be mediated by other bioactive compounds. PMID: 28368336
An imbalance in ANGPT-2, combined with related factors such as VEGF, beta-catenin, and MMP-2, may partially explain the structural derangements of the arterial wall in patients with chronic obstructive pulmonary disease. PMID: 26801565
Results demonstrate that Ang-2 expression significantly correlated with poor prognosis for patients with non-small cell lung cancer. [meta-analysis] PMID: 27589869
Our results show that miR-181a is down-regulated in glioblastoma multiforme (GBM) patients. The three target genes, ANGPT2, ARHGAP18 and LAMC1, are negatively correlated with the expression of miR-181a. Moreover, high expression of ANGPT2 or LAMC1 together with large size of GBM is correlated with a shorter median overall survival PMID: 27176932
These results suggested that IL-35 restrains rheumatoid arthritis angiogenesis and inflammation by downregulating basal and VEGF-induced Ang2 secretion as well as disrupting Ang2/Tie2 signal transduction. PMID: 27960151
This study identified Ang-2 as an endothelial cell-derived regulator of BBB permeability. PMID: 26932603
Serum Ang-2 may be a relevant predictor of Acute Pancreatitis severity, in particular of the development of AP-renal syndrome. PMID: 27022209
Data show that angiopoietin-2 (Ang-2) and bacterial endotoxins (LPS) follow opposite kinetics in serum in acute pyelonephritis. PMID: 26844659
IL-19 and Ang-2 might be involved in angiogenesis of T2 Diabetes mellitus complications. PMID: 26657726
Compared with patients with heart failure or those with orthotopic heart transplantation, serum levels and endothelial expression of Ang-2 were higher in LVAD patients. Ang-2 may contribute to arteriovenous malformation formation and subsequent bleeding in LVAD patients. PMID: 27354285

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

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Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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