Recombinant Human Alanine Aminotransferase 1 (GPT) Protein (His)

Name: Recombinant Human Alanine Aminotransferase 1 (GPT) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09721P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Description	Recombinant Human Alanine Aminotransferase 1 (GPT) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P24298
Target Symbol	GPT
Synonyms	AAT1; Alanine aminotransferase 1; Alanine aminotransferase; ALAT1_HUMAN; ALT1; Glutamate pyruvate transaminase 1; Glutamic alanine transaminase 1; Glutamic pyruvate transaminase (alanine aminotransferase); Glutamic pyruvic transaminase 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT 1; gpt; GPT1
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	ASSTGDRSQAVRHGLRAKVLTLDGMNPRVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGDAQAMGQRPITFLRQVLALCVNPDLLSSPNFPDDAKKRAERILQACGGHSLGAYSVSSGIQLIREDVARYIERRDGGIPADPNNVFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELGAVQVDYYLDEERAWALDVAELHRALGQARDHCRPRALCVINPGNPTGQVQTRECIEAVIRFAFEERLFLLADEVYQDNVYAAGSQFHSFKKVLMEMGPPYAGQQELASFHSTSKGYMGECGFRGGYVEVVNMDAAVQQQMLKLMSVRLCPPVPGQALLDLVVSPPAPTDPSFAQFQAEKQAVLAELAAKAKLTEQVFNEAPGISCNPVQGAMYSFPRVQLPPRAVERAQELGLAPDMFFCLRLLEETGICVVPGSGFGQREGTYHFRMTILPPLEKLRLLLEKLSRFHAKFTLEYS
Expression Range	2-496aa
Protein Length	Full Length of Mature Protein
Mol. Weight	56.5kDa
Research Area	Metabolism
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.
Subcellular Location	Cytoplasm.
Protein Families	Class-I pyridoxal-phosphate-dependent aminotransferase family, Alanine aminotransferase subfamily
Database References	HGNC: 4552 OMIM: 138200 KEGG: hsa:2875 STRING: 9606.ENSP00000378408 UniGene: Hs.103502
Tissue Specificity	Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

Gene Functions References

a relationship between NAFLD with high ALT and lower BMD in the general U.S. population PMID: 29897928
The detection of ALT, AFP, AFP-L3, and GP73 has a certain guiding significance to predict the risk of hepatocellular carcinoma in hepatic cirrhosis patients PMID: 28540298
In men with hepatic steatosis, we found that elevated serum ALT levels were associated with lower serum SHBG levels. PMID: 28540984
Our results using Mendelian randomization suggest that ALT reduces the risk of ischaemic heart disease (IHD), probably through reducing triglyceride levels. PMID: 28007909
Measurements of serum alpha-fetoprotein (AFP) levels could be helpful in detecting progression or recurrence in treated patients with hepatitis B virus (HBV) - hepatocellular carcinoma (HCC) and normal alanine aminotransferase (ALT). PMID: 27304617
Baseline alanine transaminase (ALT) and a hepatitis B surface antigen (HBsAg) above 1000 IU/ml were independent predictive factors for ALT elevation. PMID: 27935953
High serum alanine aminotransferase expression is associated with metabolic syndrome. PMID: 27390880
The data of this study confirmed the genetic heterogeneity of childhood-onset pure HSP, with SPG4/SPAST and SPG3A/ATL1 being the most frequent forms. PMID: 27260292
Results indicate that AZD4619 increased alanine aminotransferase (ALT1) protein expression in a dose-dependent manner in human, but not in rat hepatocytes. PMID: 27430334
Liver fat content and serum ALT were inversely correlated with bone mineral density in middle-aged and elderly men. PMID: 26791408
Age, female gender, and elevated ALT can help identifying cases with biliary etiology of acute pancreatitis. PMID: 26664483
IBS [irritable bowel syndrome ] may be an important condition in certain patients with elevated ALT levels and MS [ metabolic syndrome ]. PMID: 26632395
Data suggest that, in context of abnormal hepatic lipid accumulation in nonalcoholic fatty liver disease, circulating GPT1 rises due to up-regulation of hepatic expression of GPT1 associated with greater gluconeogenesis and insulin resistance. PMID: 26791191
Elevated aminotransferase levels, even within the normal range, were significantly associated with increased risk of all external-cause mortalities, including suicide, and injury. PMID: 26446640
There are significant relationships of elevated ALT levels with cardiometabolic risk factors (obesity, hypertension, hypercholesteremia, hyperuricemia, hypertriglyceridemia) and several sex-related differences in rural Chinese. PMID: 26160405
ALT was significantly associated with elevated levels of triacylglycerols, hyperglycemia, and obesity. In patients with metabolic syndrome, one must consider liver involvement if the patient has ALT levels in the upper third of the reference range. PMID: 25874513
Peginterferon alfa-2a is associated with elevations in alanine aminotransferase at the end of treatment in chronic hepatitis C patients with sustained virologic response. PMID: 24937007
A prospective association between ALT levels and metabolic syndrome highlights the value of ALT levels, even mild ALT elevations within the normal range, as a predictor of the MetS risk in male schizophrenia patients. PMID: 24819732
Serum glutamate pyruvate transaminase levels were elevated postoperatively in recipients of an orthotopic liver transplant from a brain-dead donor receiving extracorporeal membrane oxygenation. PMID: 25543901
Statistically significant differences were observed in the mean values of GPT in type 1 diabetics compared to type 2 and control groups PMID: 24849491
Alanine aminotransferase showed associations with BMI, glycaemia, lipids, and creatinine. The relationship between alanine aminotransferase and age was best expressed by polynomial regression. PMID: 24953204
Serum alanine aminotransferase has limited predictive value for liver disease in chronic hepatitis C PMID: 25219834
Role of insulin resistance and adipocytokines on serum alanine aminotransferase in obese patients with type 2 diabetes mellitus PMID: 23884827
In nonalcoholic fatty liver disease, adipose tissue insulin resistance (but not hepatic insulin resistance) and liver triglyceride content are major factors in the elevation of plasma aminotransferase levels. PMID: 25145475
elevated ALT is common in the Korean population; it is associated with metabolic disorders (obesity, insulin resistance, or metabolic syndrome) in the majority of patients PMID: 24275711
ALT and visceral adipose tissue affect metabolic risk factors in a middle-aged Japanese population PMID: 24500143
PNPLA3 I148M polymorphism is associated with elevated alanine transaminase levels in Mexican indigenous and Mestizo populations. PMID: 24691744
Among patients with chronic HCV infection, daily consumption of filtered coffee may have a beneficial effect on the stabilization of ALT levels. PMID: 24349501
A new model that combines ALT, platelets, CK-18 fragments, and triglycerides was established by logistic regression among nonalcoholic fatty liver disease patients. PMID: 24324749
Elevated ALT is associated with developing depressive symptoms, suggesting that non-alcoholic fatty liver disease may represent an independent modifiable risk factor for depression. PMID: 23522007
fibrosis is not rare in Chinese CHB patients with persistent normal alanine aminotransferase, especially HBeAg-positive patients over 30 years old PMID: 24205292
Elevated serum ALT levels are prevalent among elderly agricultural and fishing population workers in Taiwan. PMID: 24158044
Elevated serum alanine aminotransferase level was independently associated with an increased risk of intracerebral hemorrhage in East Asian men. PMID: 23880909
Normal to near-normal serum aminotransferase levels are associated with metabolic disorders. PMID: 23794308
Data from cross-sectional study in children/adolescents in Republic of Korea suggest normal ALT/GPT serum reference values; ALT/GPT serum level appears effective as biomarker in predicting cardiovascular/metabolic syndrome risk in population studied. PMID: 23403445
Among HIV/HCV-coinfected persons, risky drinking was associated with a higher adjusted mean AST (62.2 vs. 51.4 U/L; adjusted ratio of means 1.2, 95% CI [1.07, 1.37], p = .003) and ALT (51.3 vs. 41.6 U/L; compared with non-risky drinking PMID: 23384374
Nonalcoholic fatty liver disease and metabolic syndrome were associated with ALT levels in a male population from southern China. PMID: 22975640
ALT[Alanine transaminase] may be a marker of an underlying etiology relating to the paradoxical associations of diabetes and IHD at a population level. PMID: 22954960
An elevated ALT does not accurately predict significant liver injury. Decisions on commencing antiviral therapy should not be heavily based on a particular ALT threshold. PMID: 22389715
In a cross-sectional analysis of Taiwanese industrial employees, elevated ALT associated with MS, but in subjects who did not meet MS criteria, elevated ALT by itself did not associate with IFG/T2DM. PMID: 21715038
Patients with HLA-DQA1 and DRB1 genotypes have higher alanine aminotransferase elevation, whereas HLA-negative cases have a higher incidence of Gilbert's syndrome. PMID: 22357454
In a cross-sectional study, Chinese adolescents and young adults with more metabolic syndrome components were more likely to suffer from elevated ALT PMID: 22177542
alanine aminotransferase levels largely within the normal range, there was an inverse association between ALT and all-cause mortality and cardiovascular events PMID: 22158663
the COMT polymorphism affects serum ALT activity to maintain liver function PMID: 22293393
The magnitude of the association of PNPLA3 with liver enzymes is driven by the size of abdominal fat. PMID: 22140488
discriminates persons infected with hepatitis C virus from those at low risk of liver disease, but would be considered elevated in a large proportion of the U.S. population PMID: 21987480
An hepatitis B virus DNA level of >/=5 log10 copies/mL and an alanine aminotransferase (ALT) level of >20 to PMID: 21692932
The prevalence of metabolic syndrome (MS) and risk factors for and association with elevated alanine aminotransferase (ALT) as markers of hepatic injury in a large Hispanic health disparity cohort with high rates of obesity, was studied. PMID: 21720553
Subjects with metabolic syndrome had significantly higher plasma levels of alanine aminotransferase. PMID: 21040936
Nrmal levels of ALT are associated with metabolic and androgenic phenotypes, i women of reproductive age. PMID: 20961180

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.