Recombinant Human ACK Protein
Beta LifeScience
SKU/CAT #: BL-0268SG

Recombinant Human ACK Protein
Beta LifeScience
SKU/CAT #: BL-0268SG
Catalog No.: BL-0268SG
Product Overview
Tag | GST |
Host Species | Human |
Accession | NM_005781 |
Synonym | TNK2, ACK1, FLJ44758, FLJ45547, p21cdc42Hs |
Background | ACK is a tyrosine kinase that binds CDC42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of CDC42Hs (1). Overexpression of ACK in cancer cell lines of epithelial origin increases cellular motility and invasiveness. In a mouse model, ACK overexpression enhances the ability of a human breast cancer cell line to metastasize to the lung and increased mortality (2). Ligand stimulation of alpha-3 beta-1 integrin leads to activation of ACK which then enhances p130CAS phosphorylation and activation of RAC. |
Description | Recombinant human ACK (110-476) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. This protein is purified with our unique purification methods. |
Source | Sf9 insect cells |
AA Sequence | 110a.a.-476 |
Molecular Weight | ~66 kDa |
Purity | For specific purity information on a given lot, see related COA. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Active |
Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
Stability | The recombinant protein is stable for up to 12 months at -70°C |
Usage | For Research Use Only |
Storage | Recombinant Human ACK Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |