Recombinant Human 5-Hydroxytryptamine Receptor 1B (HTR1B) Protein (His-SUMO)

Name: Recombinant Human 5-Hydroxytryptamine Receptor 1B (HTR1B) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03383P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins, Recombinant proteins fall special offers - full-length proteins, Recombinant transmembrane proteins – fall special offers

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Human 5-Hydroxytryptamine Receptor 1B (HTR1B) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P28222
Target Symbol	HTR1B
Synonyms	HTR1B; HTR1DB; 5-hydroxytryptamine receptor 1B; 5-HT-1B; 5-HT1B; S12; Serotonin 1D beta receptor; 5-HT-1D-beta; Serotonin receptor 1B
Species	Homo sapiens (Human)
Expression System	in vitro E.coli expression system
Tag	N-10His-SUMO
Target Protein Sequence	MEEPGAQCAPPPPAGSETWVPQANLSSAPSQNCSAKDYIYQDSISLPWKVLLVMLLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQVVCDFWLSSDITCCTASILHLCVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISLPPFFWRQAKAEEEVSECVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPNRTGKRLTRAQLITDSPGSTSSVTSINSRVPDVPSESGSPVYVNQVKVRVSDALLEKKKLMAARERKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIFDFFTWLGYLNSLINPIIYTMSNEDFKQAFHKLIRFKCTS
Expression Range	1-390aa
Protein Length	Full Length
Mol. Weight	62.1kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	G-protein coupled receptor 1 family
Database References	HGNC: 5287 OMIM: 182131 KEGG: hsa:3351 STRING: 9606.ENSP00000358963 UniGene: PMID: 28923721 The polymorphism of HTR1B C861G is not a risk factor for Tourette's in Han Chinese population. PMID: 26123080 Acute tryptophan depletion (ATD) did not significantly influence participants' sensitivity to probability, wins, or losses, although there was a trend for a lower tendency to choose experimental gambles overall following depletion. Following ATD, participants with higher peripheral 5-HT1B transcription levels increased choice of experimental gambles and used information pertaining to possible wins more when making choices PMID: 27638901 This study found several promoter variants in linkage disequilibrium that may potentially influence the allelic imbalance in the C861G variant. aAnd no evidence of allelic imbalance nor parent-of-origin effects of the C861G variant was observed in suicidal behavior. PMID: 28445878 Single nucleotide polymorphism in HTR1B gene is associated with Attention deficit hyperactivity disorder. PMID: 28512748 nine ADHD candidate single nucleotide polymorphisms (SNPs) in seven genes were tested for association with PD in 5333 cases and 12,019 healthy controls. No significant association was observed. PMID: 28176268 results highlight the importance of HTR1 in leukemogenesis and LSC survival and identify this receptor family as a new target for therapy in AML with prognostic value PMID: 28193998 HTR polymorphisms related to vulnerability to major depressive disorder (MDD) and MDD with suicidal attempts. PMID: 28007644 The results of this study give new evidence for the role of epigenetic and genetic factors which could modulate HTR1B expression in the pharmacological response to antidepressants. PMID: 28025020 5-HT1B receptor-dependent cellular Src-related kinase-Nox1-pathways contribute to vascular remodeling in pulmonary arterial hypertension. PMID: 28473438 preliminary findings suggest a role of the HTR1B gene in susceptibility to development of bulimia nervosa subtypes. Furthermore, this gene might have an impact on the severity of anxiety in anorexia nervosa-spectrum patients. PMID: 27579596 Polymorphic variants of the HTR1B gene are associated with the susceptibility of secondary RP in vibration-exposed occupational populations of Chinese Han people. PMID: 26639766 large scale neural activation differences in the inferior and medial frontal and temporal/parietal regions of the response inhibition network between the different variants of both the HTR1B and 5HTT genes; activation in these regions was significantly associated with stop-task performance, but not with ADHD diagnosis or severity PMID: 26428549 Results from Parkinson's disease patients and MPTP-lesioned monkeys argue in favor of a contribution of 5-HT1B receptors in the pathophysiology of levodopa-induced motor complications PMID: 26254863 The interaction between two major serotonergic structures involved in serotonin release, the SERT and 5-HT1B receptor, results in a modification of the inhibitory serotonergic tone mediated via 5-HT1A receptors. PMID: 25652393 Serotonin 1B Receptor Gene (HTR1B) Methylation as a Risk Factor for Callous-Unemotional Traits in Antisocial Boys PMID: 25993020 Down-regulation of 5-HT1B and 5-HT1D receptors inhibits proliferation, clonogenicity and invasion of human pancreatic cancer cells. PMID: 25268648 reductions in 5-HT1B availability found in this study suggest that 5-HT1B receptors may contribute to the etiology or expression of cocaine dependence and potentially represent a target for medication development. PMID: 24433854 5-HT1B- and 5-HT1D-mediated signaling play an important role in the regulation of the proliferative and invasive phenotype of pancreatic cancer cells. PMID: 25170871 Genetic variation in HTR1B predicts changes in bone metabolism during SSRI use. PMID: 24074042 HTR1B SNPs significantly associate with early-onset obsessive compulsive disorder. PMID: 24571444 Low level of 5-HT(1B)receptors in limbic system corelates to decreased serotonin function in patients with Parkinson disease.(review) PMID: 24126162 HTR1B SNP rs6296 was associated with childhood aggressive behavior but not with adulthood anger or hostility. The HTR1B SNP rs6296 modified the association between childhood aggressive behavior and adulthood hostility. PMID: 22945537 5-HT inhibits pulmonary artery smooth muscle cell apoptosis via pERK1/2, PDK, 5-HT1B, 5-HTT pathway. PMID: 23601559 Alterations in 5HT1B-receptor density were found to be differentially linked to a novel, five-factor model of posttraumatic stress disorder symptoms. PMID: 22584868 An association between the sexual dimension of aging males' syndrome and genetic variants of 5-HTR1B G861C is observed. PMID: 23485949 Brain 5-HT1B receptor occupancy with zolmitriptan is demonstrated. PMID: 23430984 In a Turkish population of migraine patients no genetic association with 5-HT1B polymorphisms is found. PMID: 23375453 These results cannot provide support for an important function of TPH1 and HTR1B in the pathogenesis of sclerosing bone dysplasias. PMID: 23563356 This meta-analysis supports the associations of HTR1B -261T>G and -161A>T with alcohol and drug abuse and further investigations are warranted in larger samples. PMID: 23335468 The results of this study concluded that SSRIs reduce attention in older adults, particularly in those with high-expression genetic variants at the 1B receptors. PMID: 22717018 HTR1B genotypes AA & AT are associated with a lower age of onset of smoking in women than the TT genotype. PMID: 23177301 crystal structures of the 5-HT1B G protein-coupled receptor bound to the agonist antimigraine medications ergotamine and dihydroergotamine PMID: 23519210 Epistatic interactions between presynaptic components (5HTR1B and SLC6A4) as reported are likely to regulate serotonin levels in the synapse and impact treatment for ADHD. PMID: 22575343 The CC genotype of HTR1B G681C polymorphism had higher cortisol and prolactin responses and higher platelet-rich plasma serotonin in health volunteers but this was not observed in nonresponsive OCD with the same genotype after chronic SRI treatment. PMID: 22522758 age is a relevant factor for 5-HT(1B) in the cortex of healthy adults PMID: 22851636 Preliminary data imply that the genetic variants of the 5-HT1B receptor might be associated with prostate cancer PMID: 22420486 Neither the 5-HTTLPR gene nor the 5-HT1B G861C polymorphism alone is a risk factor for antisocial alcoholism in Taiwan's Han Chinese population, but that the interaction between both genes may increase susceptibility to antisocial alcoholism. PMID: 22550993 our findings point to an association between functional variants in the promoter region of the HTR1B gene and alcohol dependence, supporting previous neurobiological evidences of the involvement of HTR1B variations in alcohol-related phenotypes. PMID: 22005095 two SNPs, both serotonin receptor 5-HT(1B) linked, had a significant main effect on ejaculation latency time. PMID: 22240001 Selective inhibition of 5-HT2A by sarpogrelate stimulated angiogenesis, restoring ischemic limb blood perfusion in a severe diabetic mouse model through stimulation of the eNOS/Akt pathway via the endothelial 5-HT1B receptor. PMID: 22172591 There was a statistically significant association with Body Mass Index at HTR1B single nucleotide polymorphism rs4140535 in African Americans but not in Caucasians. PMID: 22106445 genetic association studies in Ireland/Northern Ireland: SNPs in HTR1B and OPRmu1 genes are associated with subgroups of subjects with alcohol dependence distinguished by age of onset. PMID: 21906503 These findings point to a role for HTR(1B) polymorphism in heroin dependence among Han Chinese, and may be informative for future genetic or neurobiological studies on heroin dependence. PMID: 21839728 Sumatriptan and tegaserod were more potent at the 124Cys-variant h5-HT1B receptor PMID: 21478802 findings suggest that allelic variability in SLC1A2/3, 5-HTR1B and NTRK2 may be relevant to the underlying diathesis for suicidal acts. PMID: 21711518 The HTR1B A-161T polymorphism may be particularly valuable as a functional genetic marker for alcoholis PMID: 21172311 our findings suggest abnormally reduced function of ventral striatum/ventral pallidum 5-HT1B receptors in humans with major depressive disorder PMID: 20480149 This study found haplotype of HTR1B gene that was significantly more frequent in worman then in mam. PMID: 20674037 There was no assocition of HTR1B with adolescent idiopathic scoliosis. PMID: 20388441

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.