Recombinant E.Coli Rna Polymerase Sigma Factor Rpoh (RPOH) Protein (His&Myc)

Name: Recombinant E.Coli Rna Polymerase Sigma Factor Rpoh (RPOH) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-05053P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant E.Coli Rna Polymerase Sigma Factor Rpoh (RPOH) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P0AGB3
Target Symbol	RPOH
Synonyms	rpoH; fam; hin; htpR; b3461; JW3426; RNA polymerase sigma factor RpoH; Heat shock regulatory protein F33.4; RNA polymerase sigma-32 factor
Species	Escherichia coli (strain K12)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYHGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
Expression Range	1-284aa
Protein Length	Full Length
Mol. Weight	39.9 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes. Intracellular concentration of free RpoH protein increases in response to heat shock, which causes association with RNA polymerase (RNAP) and initiation of transcription of heat shock genes, including numerous global transcriptional regulators and genes involved in maintaining membrane functionality and homeostasis. RpoH is then quickly degraded, leading to a decrease in the rate of synthesis of heat shock proteins and shut-off of the heat shock response.
Subcellular Location	Cytoplasm.
Protein Families	Sigma-70 factor family, RpoH subfamily
Database References	KEGG: ecj:JW3426 STRING: 316385.ECDH10B_3635

Gene Functions References

ThiS, an ubiquitin-like protein, is covalently attached to delta(32) and at least 27 other proteins, leading to their subsequent degradation by proteases, in a similar manner to the ubiquitin-proteasome system (UPS) in eukaryotes. PMID: 25721662
co-opts the signal recognition particle to regulate protein homeostasis PMID: 24358019
Authors conclude that oxidative stress triggers the heat shock response in Escherichia coli sigma(32) dependently. PMID: 23585533
N-terminal half of region 2.1 is required for modulating both metabolic stability and the activity of sigma32 PMID: 15516558
First systematic study of the sigma 32 regulon in E. coli including sigma 32 protein level determination gives insight into the complex network regulated by sigma 32 and how it contributes physiological adaptation to changes in the external environment. PMID: 15757896
response protects the cell membrane and responds to its status: Fully 25% of sigma32 regulon members reside in the membrane and alter its functionality; a disproportionate fraction of overexpressed proteins that induce the response are membrane localized PMID: 16818608
Mutants identify a regulatory step downstream of chaperone binding that is required for both inactivation and degradation of sigma32. PMID: 17968012
sigma32-mediated gene expression is a general response to protein insolubility in E. coli PMID: 17992580
it is deduced from these data that there may be a link between sigma(32) regulon activity and cell division events PMID: 19114495
The -10 region in sigma32 is a composite element required for high promoter activity. PMID: 19400791

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.