Recombinant E.Coli Dna Ligase (LIGA) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-10080P

Greater than 90% as determined by SDS-PAGE.
Recombinant E.Coli Dna Ligase (LIGA) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-10080P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant E.Coli Dna Ligase (LIGA) Protein (His) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P15042 |
Target Symbol | LIGA |
Synonyms | ligA; dnaL; lig; lop; pdeC; b2411; JW2403; DNA ligase; EC 6.5.1.2; Polydeoxyribonucleotide synthase [NAD(+)] |
Species | Escherichia coli (strain K12) |
Expression System | E.coli |
Tag | N-6His |
Target Protein Sequence | MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS |
Expression Range | 1-671aa |
Protein Length | Full Length |
Mol. Weight | 77.6kDa |
Research Area | Others |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
Protein Families | NAD-dependent DNA ligase family, LigA subfamily |
Database References | KEGG: ecj:JW2403 STRING: 316385.ECDH10B_2576 |
Gene Functions References
- Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
- Here the authors show that replication without ligase kills after a short resistance period. PMID: 22582878
- temperature sensitive mutant characterized PMID: 15583169
- analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
- The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
- 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
- structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356