Recombinant Cynomolgus NKG2C&CD94 Protein

Name: Recombinant Cynomolgus NKG2C&CD94 Protein
Brand: Beta LifeScience
SKU: BLK-01587P-100UG
Availability: InStock

Cynomolgus NKG2C&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: All products, High-quality recombinant proteins

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Product Overview

Description	Recombinant Cynomolgus NKG2C&CD94 Protein is expressed from HEK293 with His tag at the N-Terminus and Flag tag at the C-Terminus.It contains Glu98-Leu231(NKG2C)&Lys32-Ile179(CD94).
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Accession	Q68VD0(NKG2C)&Q68VD4(CD94)
Target Symbol	NKG2C&CD94
Synonyms	NKG2C&CD94
Species	Cynomolgus
Expression System	HEK293
Tag	N-His, C-Flag
Expression Range	Glu98-Leu231(NKG2C) & Lys32-Ile179(CD94)
Mol. Weight	The protein has a predicted MW of 16.44kDa (NKG2C) & 18.27kDa (CD94). Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.