Recombinant CRISPR-Cas13a Nuclease

Beta LifeScience SKU/CAT #: BLC-0299P

Recombinant CRISPR-Cas13a Nuclease

Beta LifeScience SKU/CAT #: BLC-0299P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Background Cas13a nuclease (also known as C2c2) is an effector protein of the Type VI CRISPR-Cas system, possessing RNA-mediated RNA endonuclease activity. It can specifically cleave target RNA in the presence of a Protospacer Flanking Site (PFS) sequence on the target single-stranded RNA. Additionally, Cas13a exhibits collateral cleavage activity (i.e., non-targeted cleavage activity) that is dependent on the presence of the target single-stranded RNA, which can be utilized to develop rapid diagnostic assays for targeted nucleic acids.
Description We have developed a Cas13a Nuclease derived from the bacterium Leptotrichia wadei (Lwa), which is recombinantly expressed in E. coli with a purity exceeding 95%. It can be used for in vitro RNA cleavage, in vitro RNA detection, as well as for the regulation of RNA within living cells and RNA genome editing. Furthermore, it can also be used for labeling in optical probe biology. In vitro, Cas13a stably binds to crRNA, and by introducing Cas13a/crRNA (or crRNA plasmid) into cells, it is possible to achieve RNA editing within the cells. Cas13a/crRNA can also be activated by target RNA, enabling highly sensitive RNA detection outside of cells.
Source E.coli
Purity >95% by SDS-PAGE and SEC-HPLC
Endotoxin < 100EU/mg
Component and Size
Component S L
LwaCas13a Nuclease (5pmol/μl) 40μl 200μl
10×Cas13a Reaction Buffer 1ml 1ml×5
Quality Control After multiple column purifications, SDS-PAGE gel analysis shows only a clear, single target band, indicating a purity of 95%. The endotoxin content is less than 100 EU/mg. qPCR analysis reveals no residual E. coli genomic contamination, and there is no contamination with RNase or nucleic acid endo- and exonucleases.
Usage For Research Use Only
Storage Store, frozen at -70°C for longer periods of time. Avoid repeated freeze-thaw cycles.


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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