Recombinant CRISPR-Cas13a protein

Name: Recombinant CRISPR-Cas13a Nuclease
Brand: Beta LifeScience
SKU: BLC-0299P
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Background

Cas13a nuclease (also known as C2c2) is an effector protein of the Type VI CRISPR-Cas system, possessing RNA-mediated RNA endonuclease activity. It can specifically cleave target RNA in the presence of a Protospacer Flanking Site (PFS) sequence on the target single-stranded RNA. Additionally, Cas13a exhibits collateral cleavage activity (i.e., non-targeted cleavage activity) that is dependent on the presence of the target single-stranded RNA, which can be utilized to develop rapid diagnostic assays for targeted nucleic acids.

Description

We have developed a Cas13a Nuclease derived from the bacterium Leptotrichia wadei (Lwa), which is recombinantly expressed in E. coli with a purity exceeding 95%. It can be used for in vitro RNA cleavage, in vitro RNA detection, as well as for the regulation of RNA within living cells and RNA genome editing. Furthermore, it can also be used for labeling in optical probe biology. In vitro, Cas13a stably binds to crRNA, and by introducing Cas13a/crRNA (or crRNA plasmid) into cells, it is possible to achieve RNA editing within the cells. Cas13a/crRNA can also be activated by target RNA, enabling highly sensitive RNA detection outside of cells.

Source

E.coli

Purity

>95% by SDS-PAGE and SEC-HPLC

Endotoxin

< 100EU/mg

Component and Size

Component	S	L
LwaCas13a Nuclease (5pmol/μl)	40μl	200μl
10×Cas13a Reaction Buffer	1ml	1ml×5

Quality Control

After multiple column purifications, SDS-PAGE gel analysis shows only a clear, single target band, indicating a purity of 95%. The endotoxin content is less than 100 EU/mg. qPCR analysis reveals no residual E. coli genomic contamination, and there is no contamination with RNase or nucleic acid endo- and exonucleases.

Usage

For Research Use Only

Storage

Store, frozen at -70°C for longer periods of time. Avoid repeated freeze-thaw cycles.

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.