Recombinant Bovine Acetylcholinesterase (ACHE) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-09976P

Greater than 90% as determined by SDS-PAGE.
Recombinant Bovine Acetylcholinesterase (ACHE) Protein (GST)
Beta LifeScience
SKU/CAT #: BLC-09976P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Bovine Acetylcholinesterase (ACHE) Protein (GST) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P23795 |
Target Symbol | ACHE |
Synonyms | ACHEAcetylcholinesterase; AChE; EC 3.1.1.7 |
Species | Bos taurus (Bovine) |
Expression System | E.coli |
Tag | N-GST |
Target Protein Sequence | EGPEDPELLVMVRGGRLRGLRLMAPRGPVSAFLGIPFAEPPVGPRRFLPPEPKRPWPGVLNATAFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPSSPTPVLVWIYGGGFYSGASSLDVYDGRFLTQAEGTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGVGEARRRATLLARLVGCPPGGAGGNDTELVACLRARPAQDLVDHEWRVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAQFLAGVRVGVPQASDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLSWPLWMGVPHGYEIEFIFGLPLEPSLNYTIEERTFAQRLMRYWANFARTGDPNDPRDPKAPQWPPYTAGAQQYVSLNLRPLEVRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQDRCSDL |
Expression Range | 31-613aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 91.5kDa |
Research Area | Others |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. |
Subcellular Location | Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.; [Isoform H]: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. |
Protein Families | Type-B carboxylesterase/lipase family |
Database References |
Gene Functions References
- To date, AChE and BChE are the only proteins known that include polyproline tetramer organizing peptides in their tetrameric structure. PMID: 23352838
- The monomeric form of AChE in fetal bovine serum is trimmed at the carboxyl-terminus compared to the tetrameric form, consistent with the involvement of C-terminal amino acids in the assembly of monomers into tetramers. PMID: 14690439
- findings show that AChE induces a remarkable aggregation of PrP 106-126 with a mechanism similar to that described for amyloid beta protein PMID: 16750169
- In this work, the catalytic activity of acetylcholinesterase (AChE) immobilized on these materials was investigated, using neostigmina as AChE inhibitor. PMID: 17208293
- Results strongly support the role of acetylcholinesterase in triggering amyloidogenesis and the potential therapeutic relevance of peripheral site blocker compounds. PMID: 19038345
- Hydrolysis of acetylthiocoline, o-nitroacetanilide and o-nitrotrifluoroacetanilide by fetal bovine serum acetylcholinesterase PMID: 19292875