Recombinant Bombyx Mori Prothoracicotropic Hormone (PTTH) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-09791P

Greater than 90% as determined by SDS-PAGE.
Recombinant Bombyx Mori Prothoracicotropic Hormone (PTTH) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-09791P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Bombyx Mori Prothoracicotropic Hormone (PTTH) Protein (His) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | P17219 |
Target Symbol | P17219 |
Synonyms | ; Prothoracicotropic hormone; PTTH) [Cleaved into: P2K; P6K; Prothoracicotropic hormone] |
Species | BOMMO(Silk moth) |
Expression System | E.coli |
Tag | N-6His |
Target Protein Sequence | GNIQVENQAIPDPPCTCKYKKEIEDLGENSVPRFIETRNCNKTQQPTCRPPYICKESLYSITILKRRETKSQESLEIPNELKYRWVAESHPVSVACLCTRDYQLRYNNN |
Expression Range | 116-224aa |
Protein Length | Full Length of Mature Protein |
Mol. Weight | 16.7kDa |
Research Area | Others |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | PTTH is a brain secretory polypeptide of insects which stimulates the prothoracic glands to produce and release ecdysone, the steroid essential to insect development.; Peptides P2K and P6K are presumed to be cleaved post-translationally and may play some unknown physiologically or developmentally important functions. |
Database References | KEGG: bmor:692767 UniGene: Bmo.125 |
Tissue Specificity | PTTH is synthesized by two dorsolateral neurosecretory cells of the Bombyx brain. |
Gene Functions References
- HR38 is a prothoracicotropic hormone(PTTH)-stimulated immediate early gene that is, at least in part, induced through Ca(2+)/ERK and PI3K signaling. The present study proposes a potential cross talk mechanism between PTTH and ecdysone signaling to regulate insect development and lays a foundation for a better understanding of the mechanisms of PTTH's actions. PMID: 27090809
- There are interconnections among Ca(2+) signaling, adenosine 5'-monophosphate-activated protein kinase, and 4E-BP phosphorylation in in prothoracicotropic hormone (PTTH-activated prothoracic glands of Bombyx mori. PMID: 27751888
- Study reports the physical basis of neurohormone PTTH sensing by the receptor tyrosine kinase Torso. Hormone binding by Torso is asymmetric and anticooperative, allowing receptor levels to determine the nature of signal transduction. PMID: 26698662
- To examine the expression of Rab proteins and neuropeptide hormones in the brain of the silkworm, Bombyx mori.The Rab1 co-localized with prothoracicotropic hormone. PMID: 22922733
- PTTH/PI3K/TOR/4E-BP (S6K) signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands. PMID: 22227406
- The authors showed that Bombyx mori prothoracicotropic hormone (PTTH)-stimulated ecdysteroidogenesis was partially blocked by LY294002 and wortmannin, indicating that phosphoinositide 3-kinase (PI3K) is involved in PTTH-stimulated ecdysteroidogenesis. PMID: 21199670
- Study showed that prothoracicotropic hormone (PTTH) stimulates a rapid increase in tyrosine phosphorylation of at least 2 proteins in prothoracic glands: extracellular signal-regulated kinase and a 120-kDa protein. PMID: 20568296
- the TATA box is involved in regulation of PTTH gene expression in B. mori PMID: 20563654
- PTTH-stimulated ERK phosphorylation is only partially Ca(2+)- and calmodulin-dependent, and HNMPA-(AM)(3)-sensitive receptor tyrosine kinase is involved in activation of ERK phosphorylation by PTTH. PMID: 19800889