Recombinant Arabidopsis Thaliana Transcription Factor Hy5 (HY5) Protein (His&Myc)

Name: Recombinant Arabidopsis Thaliana Transcription Factor Hy5 (HY5) Protein (His&Myc)
Brand: Beta LifeScience
SKU: BLC-07036P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Arabidopsis Thaliana Transcription Factor Hy5 (HY5) Protein (His&Myc) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	O24646
Target Symbol	HY5
Species	Arabidopsis thaliana (Mouse-ear cress)
Expression System	E.coli
Tag	N-10His&C-Myc
Target Protein Sequence	MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL
Expression Range	1-168aa
Protein Length	Full Length
Mol. Weight	25.9 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Transcription factor that promotes photomorphogenesis in light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in the blue light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevents the activation of light-induced genes (Probable). Acts coordinately with SPL7 to regulate the microRNA miR408 and its target genes in response to changes in light and copper conditions. Regulates the abscisic acid (ABA) signaling pathway. Also involved in root gravitropism. Involved in the repression of hypocotyl cell elongation to promote photomorphogenesis. Recruits the histone deacetylase HDA15 to the promoters of a subset of cell wall organization and auxin signaling-related genes. HDA15 represses their transcription by decreasing the levels of histone H4 acetylation in a light-dependent manner.
Subcellular Location	Nucleus.
Protein Families	BZIP family
Database References	KEGG: ath:AT5G11260 STRING: 3702.AT5G11260.1 UniGene: PMID: 28167764 Repression of MYBL2 by Both microRNA858a and HY5 Leads to the Activation of Anthocyanin Biosynthetic Pathway in Arabidopsis PMID: 27450422 Prefoldins Negatively Regulate Cold Acclimation in Arabidopsis thaliana by Promoting Nuclear Proteasome-Mediated HY5 Degradation PMID: 28412546 BBX21 binds to the T/G-box in the HY5 promoter and directly activates HY5 expression in the light. PMID: 27325768 Arabidopsis ELONGATED HYPOCOTYL5 (HY5) is a shoot-to-root mobile signal that mediates light promotion of root growth and nitrate uptake. Shoot-derived HY5 auto-activates root HY5 and also promotes root nitrate uptake by activating NRT2.1, a gene encoding a high-affinity nitrate transporter. PMID: 26877080 This study showed that HY5 directly binds to the MYBD promoter and that although MYBD and MYBH are homologs, they act in opposite ways during plant photomorphogenesis. PMID: 26576746 the physical connection between brassinosteroid and light signaling pathways in regulating cotyledon opening in Arabidopsis is mediated by a direct interaction between BZR1 and HY5 PMID: 26363272 Compromised activity of HY5 may have been mainly responsible for the partial reversal of the det1 phenotype in ted3 det1. PMID: 25248106 Ultraviolet-B causes unilateral accumulation of HY5, which then, in the end, leads to bending toward the light. PMID: 25096978 Data indicate that the bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) is enriched at target promoters in response to UV-B in a UV RESISTANCE LOCUS8 (UVR8) photoreceptor-dependent manner. PMID: 25351492 by directly targeting a common promoter cis-element (G-box), HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. PMID: 24922306 our investigation demonstrates that the COP1-HY5 complex is a novel integrator that plays an essential role in ethylene-promoted hypocotyl growth in the light PMID: 24348273 A possible interplay between PFT1 and another transcription factor, HY5, may regulate induction of APS reductase expression by light. PMID: 24583010 HY5 transmits phyA signals through an FHY1/FHL-independent pathway but it may also modulate FHY1/FHL signal through its interaction with HFR1 and LAF1. PMID: 23503597 Bifurcate regulation of anthocyanin biosynthesis by HY5 via transcriptional activation of PAP1. PMID: 23583450 The HY5-AtERF11 regulon is a key factor modulating abscisic acid-regulated ethylene biosynthesis. PMID: 21645149 HSP90 proteins respond to the tetrapyrrole-mediated plastid signal to control expression of photosynthesis-associated nuclear genes PMID: 22201048 HY5 and BBX24 act antagonistically in UV-B response. PMID: 22410790 HY5 binds to over 9000 genes, detectably affecting the expression of over 1100 genes, either positively or negatively. HY5 indirectly regulate many other genes through sub-networks mediated by other regulators. PMID: 21265889 HY5 represses the expression of PKS4 and auxin-related genes. PMID: 21848684 The genetic interaction between HY5 and FRY1 indicates that HY5 and FRY1 may act in overlapping pathways that mediate light signaling and lateral root development. PMID: 21301222 HY5 directly binds to C/G-box and G-box in the HTL promoter and regulates the expression of HTL. PMID: 20864454 Data show that HY5 plays a role in negative feedback regulation of phyA signaling by attenuating FHY3/FAR1-activated FHY1/FHL expression, providing a mechanism for fine-tuning phyA signaling homeostasis. PMID: 21097709 Data show that phosphorylation-mimicking serine substitutions strongly interfere with the DNA binding of two prototypical Arabidopsis bZIPs, namely AtZIP63 and HY5. PMID: 20047775 Det1 esp1 spa1 double mutant showed higher HY5 protein levels than either single mutant or the wild type. PMID: 20041285 HY5 is required for the transcriptional activation of the PFG1/MYB12 and PFG3/MYB111 genes under UV-B and visible light. PMID: 19895401 the first structural and biophysical characterization of HY5 is reported here. PMID: 17001643 HY5 is a high hierarchical regulator of the transcriptional cascades for photomorphogenesis. PMID: 17337630 necessary for high nitrate reductase expression in far-red light PMID: 17929051 Gibberellins signaling regulates protein stability of HY5, and the activity of PIF3. PMID: 18053005 Data show that at part of the remodeling of light signaling networks involves converting HY5, a positive regulator of PhANGs, into a negative regulator of PhANGs. PMID: 18065688 Arabidopsis HY5 functions as a novel DNA-binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate PMID: 18082144 The isolation and characterization of light-regulated zinc finger protein 1 (LZF1), a HY5-regulated factor, is reported. PMID: 18182030 action spectrum for the induction of HY5 by the UVR8 pathway showed a main peak at 280 nm with a smaller peak at 300 nm PMID: 19558421

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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