Recombinant Arabidopsis Thaliana Protein Abscisic Acid-Insensitive 5 (ABI5) Protein (His-SUMO)

Name: Recombinant Arabidopsis Thaliana Protein Abscisic Acid-Insensitive 5 (ABI5) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-10013P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Arabidopsis Thaliana Protein Abscisic Acid-Insensitive 5 (ABI5) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q9SJN0
Target Symbol	ABI5
Synonyms	ABI5; BZIP39; DPBF1; GIA1; NEM1; At2g36270; F2H17.12Protein ABSCISIC ACID-INSENSITIVE 5; Dc3 promoter-binding factor 1; AtDPBF1; Protein GROWTH-INSENSITIVITY TO ABA 1; bZIP transcription factor 39; AtbZIP39
Species	Arabidopsis thaliana (Mouse-ear cress)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MVTRETKLTSEREVESSMAQARHNGGGGGENHPFTSLGRQSSIYSLTLDEFQHALCENGKNFGSMNMDEFLVSIWNAEENNNNQQQAAAAAGSHSVPANHNGFNNNNNNGGEGGVGVFSGGSRGNEDANNKRGIANESSLPRQGSLTLPAPLCRKTVDEVWSEIHRGGGSGNGGDSNGRSSSSNGQNNAQNGGETAARQPTFGEMTLEDFLVKAGVVREHPTNPKPNPNPNQNQNPSSVIPAAAQQQLYGVFQGTGDPSFPGQAMGVGDPSGYAKRTGGGGYQQAPPVQAGVCYGGGVGFGAGGQQMGMVGPLSPVSSDGLGHGQVDNIGGQYGVDMGGLRGRKRVVDGPVEKVVERRQRRMIKNRESAARSRARKQAYTVELEAELNQLKEENAQLKHALAELERKRKQQYFESLKSRAQPKLPKSNGRLRTLMRNPSCPL
Expression Range	1-442aa
Protein Length	Full Length
Mol. Weight	63.0kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. Can also trans-activate its own promoter, suggesting that it is autoregulated. Plays a role in sugar-mediated senescence.
Subcellular Location	Nucleus.
Protein Families	BZIP family, ABI5 subfamily
Database References	KEGG: ath:AT2G36270 STRING: 3702.AT2G36270.1 UniGene: At.14114
Tissue Specificity	Predominantly expressed in seeds.

Gene Functions References

SOM is reported to negatively regulate seeds germination by altering GA/ABA metabolism, here we found that AFP2 and ABI5 altered SOM transcription. Data demonstrate that AFP2 is a novel regulator to control high temperature-induced secondary seed dormancy through ABI5 and SOM. PMID: 29723526
These data demonstrate that PHO1 is involved in ABA-mediated seed germination and early seedling development and transcriptionally regulated by ABI5. PMID: 29089393
Arabidopsis MADS-Box Transcription Factor AGL21 Acts as Environmental Surveillance of Seed Germination by Regulating ABI5 Expression PMID: 28438576
ABI5 is a direct target gene of BZR1, and modulating the expression of ABI5 by BZR1 plays important roles in regulating the crosstalk between the brassinosteroid and abscisic acid signalling pathways. PMID: 27149247
NF-YC9 mediates abscisic acid (ABA) signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5. PMID: 28924726
All of the study findings suggest that ABI5 regulates seed germination at least partly by affecting ROS homeostasis. PMID: 28391398
These results suggested important roles of CRWNs in ABI5 nuclear body organization and ABI5 protein degradation during seed germination. PMID: 26564029
DOG1 affects the expression of hundreds of genes including LATE EMBRYOGENESIS ABUNDANT and HEAT SHOCK PROTEIN genes which are affected by DOG1 partly via control of ABI5 expression. PMID: 26729600
ABI5 bind to the ABR promoter, indicating that ABI5 directly regulates the expression of ABR. The disruption of ABI5 function in abr abi5-1 plants abolished the senescence-accelerating phenotype of the abr mutant, demonstrating that ABI5 is epistatic to ABR. PMID: 27095403
RGL2 promotes the entrance in secondary dormancy through ABI5 action. PMID: 26177669
link between nitric oxide and abscisic acid hormone signalling through distinct posttranslational modifications of ABI5 during early seedling development PMID: 26493030
RAV1 plays an important role in abscisic acid signaling by modulating the expression of ABI3, ABI4, and AbI5 during seed germination and early seedling development. PMID: 25231920
BIN2 phosphorylates and stabilizes ABI5 to mediate Abscisic acid (ABA) response during seed germination, while brassinosteroids (BRs) repress the BIN2-ABI5 cascade to antagonize ABA-mediated inhibition. PMID: 25415975
ubiquitination promotes AB15 degradation; sumoylation prohibits degradation PMID: 24398698
results suggest that ABA negatively regulates SHB1 expression, at least in part, through the action of its downstream signaling component ABI5 PMID: 24619610
Transgenic cotton (Gossypium hirsutum) expressing AtRAV1/2 and/or AtABI5 showed resistance to imposed drought stress. PMID: 24483851
ABI5 functions in the glucose-mediated inhibition of the root meristem zone by repressing PIN1 accumulation, thus leading to reduced auxin levels in roots. PMID: 24237322
our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in Arabidopsis thaliana. PMID: 24586210
These results define a genetic pathway through which cytokinin specifically induces the degradation of ABI5 protein. PMID: 24443524
Overexpression of TAP46 and inhibition of activities of PP2A or PP2A-like protein phosphatases can increase transcript levels of several ABI5-regulated genes. PMID: 24357600
The function of AtSAG in abscisic acid (ABA)signalling depended on ABI3 and ABI5. AtSAG is an important negative regulator of ABA signalling during seed germination and seedling development. PMID: 24163287
These data suggest that Ala214 in ABI5 contributes to the function of ABI5 via its interaction with ABI3. PMID: 23299338
ABI5 phosphorylation is regulated by PP6 phosphatase. PMID: 23404889
Data indicate that KEEP ON GOING (KEG) E3 interacts with abscisic acid insensitive 5 (ABI5) in the cytoplasm. PMID: 23720747
The inhibitory effect of abscisic acid on floral transition is mediated by ABI5. PMID: 23307919
MED25 physically associates with the basic Leu zipper transcription factor ABA-INSENSITIVE5 (ABI5) in promoter regions of ABI5 target genes and shows a negative effect on ABI5-regulated gene transcription. PMID: 22822206
one-hybrid assays demonstrated synergistic action of ABI4 with ABI5 or related bZIP factors in regulating target promoters, and mutant analyses showed that ABI4 and these bZIPs share some functions PMID: 21243515
Abscisic acid promotes ABI5 accumulation by inducing the ubiquitination and proteasomal degradation of KEG. PMID: 20682837
The redundancy of function among genes in the families of ABF3 and ABI5 in A. thaliana is reported. PMID: 16247556
Results show that over-expression of ABI5 rendered phas expression ABA-independent in the presence of PvALF. PMID: 18038114
PRT6 control of germination and establishment, as exemplified by ABA and sugar sensitivity, as well as storage oil mobilization, occurs at least in part via transcription factors ABI3 and ABI5. PMID: 19255443
SIZ1-dependent sumoylation of ABI5 attenuates abscisic acid signaling PMID: 19276109

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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